Cargando…

Phylogeography Study of the Siberian Apricot (Prunus sibirica L.) in Northern China Assessed by Chloroplast Microsatellite and DNA Makers

There is evidence that a band of dry climate separated plants in East Asia into distinct northern and southern groups. However, few studies have focused on the arid belt in this region, especially with regard to plants. We analyzed genetic variation in 22 populations of Siberian apricot (Prunus sibi...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Zhe, Zeng, Yanfei, Zhang, Zhendong, Sheng, Songbai, Tian, Ju, Wu, Rongling, Pang, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702509/
https://www.ncbi.nlm.nih.gov/pubmed/29209348
http://dx.doi.org/10.3389/fpls.2017.01989
_version_ 1783281545032761344
author Wang, Zhe
Zeng, Yanfei
Zhang, Zhendong
Sheng, Songbai
Tian, Ju
Wu, Rongling
Pang, Xiaoming
author_facet Wang, Zhe
Zeng, Yanfei
Zhang, Zhendong
Sheng, Songbai
Tian, Ju
Wu, Rongling
Pang, Xiaoming
author_sort Wang, Zhe
collection PubMed
description There is evidence that a band of dry climate separated plants in East Asia into distinct northern and southern groups. However, few studies have focused on the arid belt in this region, especially with regard to plants. We analyzed genetic variation in 22 populations of Siberian apricot (Prunus sibirica L.), a temperate deciduous species distributed in this arid belt, using two chloroplast DNA (cpDNA) sequences, seven chloroplast microsatellite loci (cpSSRs), and 31 nuclear microsatellite loci (nSSRs), to study its phylogeography. Chloroplast data showed the complete fixation of two different genetic groups: the eastern and western groups. Genetic differentiation between the two groups was significant (F(ST) = 0.90925, p < 0.01). This pronounced phylogeographic break was also indicated by nSSR data, but there were disparities regarding individual populations. An asymmetric gene flow via pollen and seeds likely resulted in discordance between the present-day geography of nuclear and chloroplast lineages. There was a distinct boundary between the two large groups, which were fixed for two of the most ancestral chlorotypes. Two populations with the highest chloroplast genetic diversity were located in the Yanshan Mountains and Jinzhou, considered to be the glacial refugia. The split of chloroplasts between the eastern and western groups was estimated to have occurred ~0.1795 Ma, whereas nuclear divergence occurred approximately 13,260 years ago. Linear regression analysis showed that climatic factors (annual precipitation and annual mean temperature) had a significant correlation with mean ancestry value (P < 0.05) indicated that they were potential factors for the formation of the two groups. In addition, this boundary was a contact zone between two groups from different refugia.
format Online
Article
Text
id pubmed-5702509
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-57025092017-12-05 Phylogeography Study of the Siberian Apricot (Prunus sibirica L.) in Northern China Assessed by Chloroplast Microsatellite and DNA Makers Wang, Zhe Zeng, Yanfei Zhang, Zhendong Sheng, Songbai Tian, Ju Wu, Rongling Pang, Xiaoming Front Plant Sci Plant Science There is evidence that a band of dry climate separated plants in East Asia into distinct northern and southern groups. However, few studies have focused on the arid belt in this region, especially with regard to plants. We analyzed genetic variation in 22 populations of Siberian apricot (Prunus sibirica L.), a temperate deciduous species distributed in this arid belt, using two chloroplast DNA (cpDNA) sequences, seven chloroplast microsatellite loci (cpSSRs), and 31 nuclear microsatellite loci (nSSRs), to study its phylogeography. Chloroplast data showed the complete fixation of two different genetic groups: the eastern and western groups. Genetic differentiation between the two groups was significant (F(ST) = 0.90925, p < 0.01). This pronounced phylogeographic break was also indicated by nSSR data, but there were disparities regarding individual populations. An asymmetric gene flow via pollen and seeds likely resulted in discordance between the present-day geography of nuclear and chloroplast lineages. There was a distinct boundary between the two large groups, which were fixed for two of the most ancestral chlorotypes. Two populations with the highest chloroplast genetic diversity were located in the Yanshan Mountains and Jinzhou, considered to be the glacial refugia. The split of chloroplasts between the eastern and western groups was estimated to have occurred ~0.1795 Ma, whereas nuclear divergence occurred approximately 13,260 years ago. Linear regression analysis showed that climatic factors (annual precipitation and annual mean temperature) had a significant correlation with mean ancestry value (P < 0.05) indicated that they were potential factors for the formation of the two groups. In addition, this boundary was a contact zone between two groups from different refugia. Frontiers Media S.A. 2017-11-21 /pmc/articles/PMC5702509/ /pubmed/29209348 http://dx.doi.org/10.3389/fpls.2017.01989 Text en Copyright © 2017 Wang, Zeng, Zhang, Sheng, Tian, Wu and Pang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Zhe
Zeng, Yanfei
Zhang, Zhendong
Sheng, Songbai
Tian, Ju
Wu, Rongling
Pang, Xiaoming
Phylogeography Study of the Siberian Apricot (Prunus sibirica L.) in Northern China Assessed by Chloroplast Microsatellite and DNA Makers
title Phylogeography Study of the Siberian Apricot (Prunus sibirica L.) in Northern China Assessed by Chloroplast Microsatellite and DNA Makers
title_full Phylogeography Study of the Siberian Apricot (Prunus sibirica L.) in Northern China Assessed by Chloroplast Microsatellite and DNA Makers
title_fullStr Phylogeography Study of the Siberian Apricot (Prunus sibirica L.) in Northern China Assessed by Chloroplast Microsatellite and DNA Makers
title_full_unstemmed Phylogeography Study of the Siberian Apricot (Prunus sibirica L.) in Northern China Assessed by Chloroplast Microsatellite and DNA Makers
title_short Phylogeography Study of the Siberian Apricot (Prunus sibirica L.) in Northern China Assessed by Chloroplast Microsatellite and DNA Makers
title_sort phylogeography study of the siberian apricot (prunus sibirica l.) in northern china assessed by chloroplast microsatellite and dna makers
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702509/
https://www.ncbi.nlm.nih.gov/pubmed/29209348
http://dx.doi.org/10.3389/fpls.2017.01989
work_keys_str_mv AT wangzhe phylogeographystudyofthesiberianapricotprunussibiricalinnorthernchinaassessedbychloroplastmicrosatelliteanddnamakers
AT zengyanfei phylogeographystudyofthesiberianapricotprunussibiricalinnorthernchinaassessedbychloroplastmicrosatelliteanddnamakers
AT zhangzhendong phylogeographystudyofthesiberianapricotprunussibiricalinnorthernchinaassessedbychloroplastmicrosatelliteanddnamakers
AT shengsongbai phylogeographystudyofthesiberianapricotprunussibiricalinnorthernchinaassessedbychloroplastmicrosatelliteanddnamakers
AT tianju phylogeographystudyofthesiberianapricotprunussibiricalinnorthernchinaassessedbychloroplastmicrosatelliteanddnamakers
AT wurongling phylogeographystudyofthesiberianapricotprunussibiricalinnorthernchinaassessedbychloroplastmicrosatelliteanddnamakers
AT pangxiaoming phylogeographystudyofthesiberianapricotprunussibiricalinnorthernchinaassessedbychloroplastmicrosatelliteanddnamakers