Time-course, negative-stain electron microscopy–based analysis for investigating protein–protein interactions at the single-molecule level

Several biophysical approaches are available to study protein–protein interactions. Most approaches are conducted in bulk solution, and are therefore limited to an average measurement of the ensemble of molecular interactions. Here, we show how single-particle EM can enrich our understanding of prot...

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Detalles Bibliográficos
Autores principales: Nogal, Bartek, Bowman, Charles A., Ward, Andrew B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2017
Materias:
Molecular Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702678/
https://www.ncbi.nlm.nih.gov/pubmed/28972148
http://dx.doi.org/10.1074/jbc.M117.808352

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