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Promising new vaccine candidates against Campylobacter in broilers

Campylobacter is the leading cause of human bacterial gastroenteritis in the European Union. Birds represent the main reservoir of the bacteria, and human campylobacteriosis mainly occurs after consuming and/or handling poultry meat. Reducing avian intestinal Campylobacter loads should impact the in...

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Autores principales: Meunier, Marine, Guyard-Nicodème, Muriel, Vigouroux, Estelle, Poezevara, Typhaine, Beven, Véronique, Quesne, S., Bigault, Lionel, Amelot, Michel, Dory, Daniel, Chemaly, Marianne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703506/
https://www.ncbi.nlm.nih.gov/pubmed/29176789
http://dx.doi.org/10.1371/journal.pone.0188472
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author Meunier, Marine
Guyard-Nicodème, Muriel
Vigouroux, Estelle
Poezevara, Typhaine
Beven, Véronique
Quesne, S.
Bigault, Lionel
Amelot, Michel
Dory, Daniel
Chemaly, Marianne
author_facet Meunier, Marine
Guyard-Nicodème, Muriel
Vigouroux, Estelle
Poezevara, Typhaine
Beven, Véronique
Quesne, S.
Bigault, Lionel
Amelot, Michel
Dory, Daniel
Chemaly, Marianne
author_sort Meunier, Marine
collection PubMed
description Campylobacter is the leading cause of human bacterial gastroenteritis in the European Union. Birds represent the main reservoir of the bacteria, and human campylobacteriosis mainly occurs after consuming and/or handling poultry meat. Reducing avian intestinal Campylobacter loads should impact the incidence of human diseases. At the primary production level, several measures have been identified to reach this goal, including vaccination of poultry. Despite many studies, however, no efficient vaccine is currently available. We have recently identified new vaccine candidates using the reverse vaccinology strategy. This study assessed the in vivo immune and protective potential of six newly-identified vaccine antigens. Among the candidates tested on Ross broiler chickens, four (YP_001000437.1, YP_001000562.1, YP_999817.1, and YP_999838.1) significantly reduced cecal Campylobacter loads by between 2 and 4.2 log(10) CFU/g, with the concomitant development of a specific humoral immune response. In a second trial, cecal load reductions results were not statistically confirmed despite the induction of a strong immune response. These vaccine candidates need to be further investigated since they present promising features.
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spelling pubmed-57035062017-12-08 Promising new vaccine candidates against Campylobacter in broilers Meunier, Marine Guyard-Nicodème, Muriel Vigouroux, Estelle Poezevara, Typhaine Beven, Véronique Quesne, S. Bigault, Lionel Amelot, Michel Dory, Daniel Chemaly, Marianne PLoS One Research Article Campylobacter is the leading cause of human bacterial gastroenteritis in the European Union. Birds represent the main reservoir of the bacteria, and human campylobacteriosis mainly occurs after consuming and/or handling poultry meat. Reducing avian intestinal Campylobacter loads should impact the incidence of human diseases. At the primary production level, several measures have been identified to reach this goal, including vaccination of poultry. Despite many studies, however, no efficient vaccine is currently available. We have recently identified new vaccine candidates using the reverse vaccinology strategy. This study assessed the in vivo immune and protective potential of six newly-identified vaccine antigens. Among the candidates tested on Ross broiler chickens, four (YP_001000437.1, YP_001000562.1, YP_999817.1, and YP_999838.1) significantly reduced cecal Campylobacter loads by between 2 and 4.2 log(10) CFU/g, with the concomitant development of a specific humoral immune response. In a second trial, cecal load reductions results were not statistically confirmed despite the induction of a strong immune response. These vaccine candidates need to be further investigated since they present promising features. Public Library of Science 2017-11-27 /pmc/articles/PMC5703506/ /pubmed/29176789 http://dx.doi.org/10.1371/journal.pone.0188472 Text en © 2017 Meunier et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Meunier, Marine
Guyard-Nicodème, Muriel
Vigouroux, Estelle
Poezevara, Typhaine
Beven, Véronique
Quesne, S.
Bigault, Lionel
Amelot, Michel
Dory, Daniel
Chemaly, Marianne
Promising new vaccine candidates against Campylobacter in broilers
title Promising new vaccine candidates against Campylobacter in broilers
title_full Promising new vaccine candidates against Campylobacter in broilers
title_fullStr Promising new vaccine candidates against Campylobacter in broilers
title_full_unstemmed Promising new vaccine candidates against Campylobacter in broilers
title_short Promising new vaccine candidates against Campylobacter in broilers
title_sort promising new vaccine candidates against campylobacter in broilers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703506/
https://www.ncbi.nlm.nih.gov/pubmed/29176789
http://dx.doi.org/10.1371/journal.pone.0188472
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