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GDSL lipases modulate immunity through lipid homeostasis in rice

Lipids and lipid metabolites play important roles in plant-microbe interactions. Despite the extensive studies of lipases in lipid homeostasis and seed oil biosynthesis, the involvement of lipases in plant immunity remains largely unknown. In particular, GDSL esterases/lipases, characterized by the...

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Autores principales: Gao, Mingjun, Yin, Xin, Yang, Weibing, Lam, Sin Man, Tong, Xiaohong, Liu, Jiyun, Wang, Xin, Li, Qun, Shui, Guanghou, He, Zuhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703576/
https://www.ncbi.nlm.nih.gov/pubmed/29131851
http://dx.doi.org/10.1371/journal.ppat.1006724
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author Gao, Mingjun
Yin, Xin
Yang, Weibing
Lam, Sin Man
Tong, Xiaohong
Liu, Jiyun
Wang, Xin
Li, Qun
Shui, Guanghou
He, Zuhua
author_facet Gao, Mingjun
Yin, Xin
Yang, Weibing
Lam, Sin Man
Tong, Xiaohong
Liu, Jiyun
Wang, Xin
Li, Qun
Shui, Guanghou
He, Zuhua
author_sort Gao, Mingjun
collection PubMed
description Lipids and lipid metabolites play important roles in plant-microbe interactions. Despite the extensive studies of lipases in lipid homeostasis and seed oil biosynthesis, the involvement of lipases in plant immunity remains largely unknown. In particular, GDSL esterases/lipases, characterized by the conserved GDSL motif, are a subfamily of lipolytic enzymes with broad substrate specificity. Here, we functionally identified two GDSL lipases, OsGLIP1 and OsGLIP2, in rice immune responses. Expression of OsGLIP1 and OsGLIP2 was suppressed by pathogen infection and salicylic acid (SA) treatment. OsGLIP1 was mainly expressed in leaf and leaf sheath, while OsGLIP2 showed high expression in elongating internodes. Biochemical assay demonstrated that OsGLIP1 and OsGLIP2 are functional lipases that could hydrolyze lipid substrates. Simultaneous down-regulation of OsGLIP1 and OsGLIP2 increased plant resistance to both bacterial and fungal pathogens, whereas disease resistance in OsGLIP1 and OsGLIP2 overexpression plants was significantly compromised, suggesting that both genes act as negative regulators of disease resistance. OsGLIP1 and OsGLIP2 proteins mainly localize to lipid droplets and the endoplasmic reticulum (ER) membrane. The proper cellular localization of OsGLIP proteins is indispensable for their functions in immunity. Comprehensive lipid profiling analysis indicated that the alteration of OsGLIP gene expression was associated with substantial changes of the levels of lipid species including monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG). We show that MGDG and DGDG feeding could attenuate disease resistance. Taken together, our study indicates that OsGLIP1 and OsGLIP2 negatively regulate rice defense by modulating lipid metabolism, thus providing new insights into the function of lipids in plant immunity.
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spelling pubmed-57035762017-12-08 GDSL lipases modulate immunity through lipid homeostasis in rice Gao, Mingjun Yin, Xin Yang, Weibing Lam, Sin Man Tong, Xiaohong Liu, Jiyun Wang, Xin Li, Qun Shui, Guanghou He, Zuhua PLoS Pathog Research Article Lipids and lipid metabolites play important roles in plant-microbe interactions. Despite the extensive studies of lipases in lipid homeostasis and seed oil biosynthesis, the involvement of lipases in plant immunity remains largely unknown. In particular, GDSL esterases/lipases, characterized by the conserved GDSL motif, are a subfamily of lipolytic enzymes with broad substrate specificity. Here, we functionally identified two GDSL lipases, OsGLIP1 and OsGLIP2, in rice immune responses. Expression of OsGLIP1 and OsGLIP2 was suppressed by pathogen infection and salicylic acid (SA) treatment. OsGLIP1 was mainly expressed in leaf and leaf sheath, while OsGLIP2 showed high expression in elongating internodes. Biochemical assay demonstrated that OsGLIP1 and OsGLIP2 are functional lipases that could hydrolyze lipid substrates. Simultaneous down-regulation of OsGLIP1 and OsGLIP2 increased plant resistance to both bacterial and fungal pathogens, whereas disease resistance in OsGLIP1 and OsGLIP2 overexpression plants was significantly compromised, suggesting that both genes act as negative regulators of disease resistance. OsGLIP1 and OsGLIP2 proteins mainly localize to lipid droplets and the endoplasmic reticulum (ER) membrane. The proper cellular localization of OsGLIP proteins is indispensable for their functions in immunity. Comprehensive lipid profiling analysis indicated that the alteration of OsGLIP gene expression was associated with substantial changes of the levels of lipid species including monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG). We show that MGDG and DGDG feeding could attenuate disease resistance. Taken together, our study indicates that OsGLIP1 and OsGLIP2 negatively regulate rice defense by modulating lipid metabolism, thus providing new insights into the function of lipids in plant immunity. Public Library of Science 2017-11-13 /pmc/articles/PMC5703576/ /pubmed/29131851 http://dx.doi.org/10.1371/journal.ppat.1006724 Text en © 2017 Gao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gao, Mingjun
Yin, Xin
Yang, Weibing
Lam, Sin Man
Tong, Xiaohong
Liu, Jiyun
Wang, Xin
Li, Qun
Shui, Guanghou
He, Zuhua
GDSL lipases modulate immunity through lipid homeostasis in rice
title GDSL lipases modulate immunity through lipid homeostasis in rice
title_full GDSL lipases modulate immunity through lipid homeostasis in rice
title_fullStr GDSL lipases modulate immunity through lipid homeostasis in rice
title_full_unstemmed GDSL lipases modulate immunity through lipid homeostasis in rice
title_short GDSL lipases modulate immunity through lipid homeostasis in rice
title_sort gdsl lipases modulate immunity through lipid homeostasis in rice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703576/
https://www.ncbi.nlm.nih.gov/pubmed/29131851
http://dx.doi.org/10.1371/journal.ppat.1006724
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