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The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline

The switch from mitosis to meiosis is the key event marking onset of differentiation in the germline stem cell lineage. In Drosophila, the translational repressor Bgcn is required for spermatogonia to stop mitosis and transition to meiotic prophase and the spermatocyte state. Here we show that the m...

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Autores principales: Bailey, Alexis S, Batista, Pedro J, Gold, Rebecca S, Chen, Y Grace, de Rooij, Dirk G, Chang, Howard Y, Fuller, Margaret T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703642/
https://www.ncbi.nlm.nih.gov/pubmed/29087293
http://dx.doi.org/10.7554/eLife.26116
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author Bailey, Alexis S
Batista, Pedro J
Gold, Rebecca S
Chen, Y Grace
de Rooij, Dirk G
Chang, Howard Y
Fuller, Margaret T
author_facet Bailey, Alexis S
Batista, Pedro J
Gold, Rebecca S
Chen, Y Grace
de Rooij, Dirk G
Chang, Howard Y
Fuller, Margaret T
author_sort Bailey, Alexis S
collection PubMed
description The switch from mitosis to meiosis is the key event marking onset of differentiation in the germline stem cell lineage. In Drosophila, the translational repressor Bgcn is required for spermatogonia to stop mitosis and transition to meiotic prophase and the spermatocyte state. Here we show that the mammalian Bgcn homolog YTHDC2 facilitates a clean switch from mitosis to meiosis in mouse germ cells, revealing a conserved role for YTHDC2 in this critical cell fate transition. YTHDC2-deficient male germ cells enter meiosis but have a mixed identity, maintaining expression of Cyclin A2 and failing to properly express many meiotic markers. Instead of continuing through meiotic prophase, the cells attempt an abnormal mitotic-like division and die. YTHDC2 binds multiple transcripts including Ccna2 and other mitotic transcripts, binds specific piRNA precursors, and interacts with RNA granule components, suggesting that proper progression of germ cells through meiosis is licensed by YTHDC2 through post-transcriptional regulation.
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spelling pubmed-57036422017-11-29 The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline Bailey, Alexis S Batista, Pedro J Gold, Rebecca S Chen, Y Grace de Rooij, Dirk G Chang, Howard Y Fuller, Margaret T eLife Developmental Biology The switch from mitosis to meiosis is the key event marking onset of differentiation in the germline stem cell lineage. In Drosophila, the translational repressor Bgcn is required for spermatogonia to stop mitosis and transition to meiotic prophase and the spermatocyte state. Here we show that the mammalian Bgcn homolog YTHDC2 facilitates a clean switch from mitosis to meiosis in mouse germ cells, revealing a conserved role for YTHDC2 in this critical cell fate transition. YTHDC2-deficient male germ cells enter meiosis but have a mixed identity, maintaining expression of Cyclin A2 and failing to properly express many meiotic markers. Instead of continuing through meiotic prophase, the cells attempt an abnormal mitotic-like division and die. YTHDC2 binds multiple transcripts including Ccna2 and other mitotic transcripts, binds specific piRNA precursors, and interacts with RNA granule components, suggesting that proper progression of germ cells through meiosis is licensed by YTHDC2 through post-transcriptional regulation. eLife Sciences Publications, Ltd 2017-10-31 /pmc/articles/PMC5703642/ /pubmed/29087293 http://dx.doi.org/10.7554/eLife.26116 Text en © 2017, Bailey et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Bailey, Alexis S
Batista, Pedro J
Gold, Rebecca S
Chen, Y Grace
de Rooij, Dirk G
Chang, Howard Y
Fuller, Margaret T
The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline
title The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline
title_full The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline
title_fullStr The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline
title_full_unstemmed The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline
title_short The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline
title_sort conserved rna helicase ythdc2 regulates the transition from proliferation to differentiation in the germline
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703642/
https://www.ncbi.nlm.nih.gov/pubmed/29087293
http://dx.doi.org/10.7554/eLife.26116
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