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The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline
The switch from mitosis to meiosis is the key event marking onset of differentiation in the germline stem cell lineage. In Drosophila, the translational repressor Bgcn is required for spermatogonia to stop mitosis and transition to meiotic prophase and the spermatocyte state. Here we show that the m...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703642/ https://www.ncbi.nlm.nih.gov/pubmed/29087293 http://dx.doi.org/10.7554/eLife.26116 |
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author | Bailey, Alexis S Batista, Pedro J Gold, Rebecca S Chen, Y Grace de Rooij, Dirk G Chang, Howard Y Fuller, Margaret T |
author_facet | Bailey, Alexis S Batista, Pedro J Gold, Rebecca S Chen, Y Grace de Rooij, Dirk G Chang, Howard Y Fuller, Margaret T |
author_sort | Bailey, Alexis S |
collection | PubMed |
description | The switch from mitosis to meiosis is the key event marking onset of differentiation in the germline stem cell lineage. In Drosophila, the translational repressor Bgcn is required for spermatogonia to stop mitosis and transition to meiotic prophase and the spermatocyte state. Here we show that the mammalian Bgcn homolog YTHDC2 facilitates a clean switch from mitosis to meiosis in mouse germ cells, revealing a conserved role for YTHDC2 in this critical cell fate transition. YTHDC2-deficient male germ cells enter meiosis but have a mixed identity, maintaining expression of Cyclin A2 and failing to properly express many meiotic markers. Instead of continuing through meiotic prophase, the cells attempt an abnormal mitotic-like division and die. YTHDC2 binds multiple transcripts including Ccna2 and other mitotic transcripts, binds specific piRNA precursors, and interacts with RNA granule components, suggesting that proper progression of germ cells through meiosis is licensed by YTHDC2 through post-transcriptional regulation. |
format | Online Article Text |
id | pubmed-5703642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-57036422017-11-29 The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline Bailey, Alexis S Batista, Pedro J Gold, Rebecca S Chen, Y Grace de Rooij, Dirk G Chang, Howard Y Fuller, Margaret T eLife Developmental Biology The switch from mitosis to meiosis is the key event marking onset of differentiation in the germline stem cell lineage. In Drosophila, the translational repressor Bgcn is required for spermatogonia to stop mitosis and transition to meiotic prophase and the spermatocyte state. Here we show that the mammalian Bgcn homolog YTHDC2 facilitates a clean switch from mitosis to meiosis in mouse germ cells, revealing a conserved role for YTHDC2 in this critical cell fate transition. YTHDC2-deficient male germ cells enter meiosis but have a mixed identity, maintaining expression of Cyclin A2 and failing to properly express many meiotic markers. Instead of continuing through meiotic prophase, the cells attempt an abnormal mitotic-like division and die. YTHDC2 binds multiple transcripts including Ccna2 and other mitotic transcripts, binds specific piRNA precursors, and interacts with RNA granule components, suggesting that proper progression of germ cells through meiosis is licensed by YTHDC2 through post-transcriptional regulation. eLife Sciences Publications, Ltd 2017-10-31 /pmc/articles/PMC5703642/ /pubmed/29087293 http://dx.doi.org/10.7554/eLife.26116 Text en © 2017, Bailey et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Bailey, Alexis S Batista, Pedro J Gold, Rebecca S Chen, Y Grace de Rooij, Dirk G Chang, Howard Y Fuller, Margaret T The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline |
title | The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline |
title_full | The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline |
title_fullStr | The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline |
title_full_unstemmed | The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline |
title_short | The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline |
title_sort | conserved rna helicase ythdc2 regulates the transition from proliferation to differentiation in the germline |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703642/ https://www.ncbi.nlm.nih.gov/pubmed/29087293 http://dx.doi.org/10.7554/eLife.26116 |
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