Cargando…
The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14
Pseudomonas aeruginosa is an opportunistic pathogen that can thrive by anaerobic respiration in the lungs of cystic fibrosis patients using nitrate as terminal electron acceptor. Here, we report the identification and characterization of the small RNA PaiI in the P. aeruginosa strain 14 (PA14). PaiI...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703892/ https://www.ncbi.nlm.nih.gov/pubmed/29218039 http://dx.doi.org/10.3389/fmicb.2017.02312 |
_version_ | 1783281767044612096 |
---|---|
author | Tata, Muralidhar Amman, Fabian Pawar, Vinay Wolfinger, Michael T. Weiss, Siegfried Häussler, Susanne Bläsi, Udo |
author_facet | Tata, Muralidhar Amman, Fabian Pawar, Vinay Wolfinger, Michael T. Weiss, Siegfried Häussler, Susanne Bläsi, Udo |
author_sort | Tata, Muralidhar |
collection | PubMed |
description | Pseudomonas aeruginosa is an opportunistic pathogen that can thrive by anaerobic respiration in the lungs of cystic fibrosis patients using nitrate as terminal electron acceptor. Here, we report the identification and characterization of the small RNA PaiI in the P. aeruginosa strain 14 (PA14). PaiI is anaerobically induced in the presence of nitrate and depends on the two-component system NarXL. Our studies revealed that PaiI is required for efficient denitrification affecting the conversion of nitrite to nitric oxide. In the absence of PaiI anaerobic growth was impaired on glucose, which can be reconciled with a decreased uptake of the carbon source under these conditions. The importance of PaiI for anaerobic growth is further underlined by the observation that a paiI deletion mutant was impaired in growth in murine tumors. |
format | Online Article Text |
id | pubmed-5703892 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57038922017-12-07 The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 Tata, Muralidhar Amman, Fabian Pawar, Vinay Wolfinger, Michael T. Weiss, Siegfried Häussler, Susanne Bläsi, Udo Front Microbiol Microbiology Pseudomonas aeruginosa is an opportunistic pathogen that can thrive by anaerobic respiration in the lungs of cystic fibrosis patients using nitrate as terminal electron acceptor. Here, we report the identification and characterization of the small RNA PaiI in the P. aeruginosa strain 14 (PA14). PaiI is anaerobically induced in the presence of nitrate and depends on the two-component system NarXL. Our studies revealed that PaiI is required for efficient denitrification affecting the conversion of nitrite to nitric oxide. In the absence of PaiI anaerobic growth was impaired on glucose, which can be reconciled with a decreased uptake of the carbon source under these conditions. The importance of PaiI for anaerobic growth is further underlined by the observation that a paiI deletion mutant was impaired in growth in murine tumors. Frontiers Media S.A. 2017-11-23 /pmc/articles/PMC5703892/ /pubmed/29218039 http://dx.doi.org/10.3389/fmicb.2017.02312 Text en Copyright © 2017 Tata, Amman, Pawar, Wolfinger, Weiss, Häussler and Bläsi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Tata, Muralidhar Amman, Fabian Pawar, Vinay Wolfinger, Michael T. Weiss, Siegfried Häussler, Susanne Bläsi, Udo The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 |
title | The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 |
title_full | The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 |
title_fullStr | The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 |
title_full_unstemmed | The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 |
title_short | The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 |
title_sort | anaerobically induced srna paii affects denitrification in pseudomonas aeruginosa pa14 |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703892/ https://www.ncbi.nlm.nih.gov/pubmed/29218039 http://dx.doi.org/10.3389/fmicb.2017.02312 |
work_keys_str_mv | AT tatamuralidhar theanaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT ammanfabian theanaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT pawarvinay theanaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT wolfingermichaelt theanaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT weisssiegfried theanaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT hausslersusanne theanaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT blasiudo theanaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT tatamuralidhar anaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT ammanfabian anaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT pawarvinay anaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT wolfingermichaelt anaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT weisssiegfried anaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT hausslersusanne anaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 AT blasiudo anaerobicallyinducedsrnapaiiaffectsdenitrificationinpseudomonasaeruginosapa14 |