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The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14

Pseudomonas aeruginosa is an opportunistic pathogen that can thrive by anaerobic respiration in the lungs of cystic fibrosis patients using nitrate as terminal electron acceptor. Here, we report the identification and characterization of the small RNA PaiI in the P. aeruginosa strain 14 (PA14). PaiI...

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Autores principales: Tata, Muralidhar, Amman, Fabian, Pawar, Vinay, Wolfinger, Michael T., Weiss, Siegfried, Häussler, Susanne, Bläsi, Udo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703892/
https://www.ncbi.nlm.nih.gov/pubmed/29218039
http://dx.doi.org/10.3389/fmicb.2017.02312
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author Tata, Muralidhar
Amman, Fabian
Pawar, Vinay
Wolfinger, Michael T.
Weiss, Siegfried
Häussler, Susanne
Bläsi, Udo
author_facet Tata, Muralidhar
Amman, Fabian
Pawar, Vinay
Wolfinger, Michael T.
Weiss, Siegfried
Häussler, Susanne
Bläsi, Udo
author_sort Tata, Muralidhar
collection PubMed
description Pseudomonas aeruginosa is an opportunistic pathogen that can thrive by anaerobic respiration in the lungs of cystic fibrosis patients using nitrate as terminal electron acceptor. Here, we report the identification and characterization of the small RNA PaiI in the P. aeruginosa strain 14 (PA14). PaiI is anaerobically induced in the presence of nitrate and depends on the two-component system NarXL. Our studies revealed that PaiI is required for efficient denitrification affecting the conversion of nitrite to nitric oxide. In the absence of PaiI anaerobic growth was impaired on glucose, which can be reconciled with a decreased uptake of the carbon source under these conditions. The importance of PaiI for anaerobic growth is further underlined by the observation that a paiI deletion mutant was impaired in growth in murine tumors.
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spelling pubmed-57038922017-12-07 The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 Tata, Muralidhar Amman, Fabian Pawar, Vinay Wolfinger, Michael T. Weiss, Siegfried Häussler, Susanne Bläsi, Udo Front Microbiol Microbiology Pseudomonas aeruginosa is an opportunistic pathogen that can thrive by anaerobic respiration in the lungs of cystic fibrosis patients using nitrate as terminal electron acceptor. Here, we report the identification and characterization of the small RNA PaiI in the P. aeruginosa strain 14 (PA14). PaiI is anaerobically induced in the presence of nitrate and depends on the two-component system NarXL. Our studies revealed that PaiI is required for efficient denitrification affecting the conversion of nitrite to nitric oxide. In the absence of PaiI anaerobic growth was impaired on glucose, which can be reconciled with a decreased uptake of the carbon source under these conditions. The importance of PaiI for anaerobic growth is further underlined by the observation that a paiI deletion mutant was impaired in growth in murine tumors. Frontiers Media S.A. 2017-11-23 /pmc/articles/PMC5703892/ /pubmed/29218039 http://dx.doi.org/10.3389/fmicb.2017.02312 Text en Copyright © 2017 Tata, Amman, Pawar, Wolfinger, Weiss, Häussler and Bläsi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tata, Muralidhar
Amman, Fabian
Pawar, Vinay
Wolfinger, Michael T.
Weiss, Siegfried
Häussler, Susanne
Bläsi, Udo
The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14
title The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14
title_full The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14
title_fullStr The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14
title_full_unstemmed The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14
title_short The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14
title_sort anaerobically induced srna paii affects denitrification in pseudomonas aeruginosa pa14
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703892/
https://www.ncbi.nlm.nih.gov/pubmed/29218039
http://dx.doi.org/10.3389/fmicb.2017.02312
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