Cargando…
Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation
A bipolar (BP) nanosecond electric pulse (nsEP) exposure generates reduced calcium influx compared to a unipolar (UP) nsEP. This attenuated physiological response from a BP nsEP exposure is termed “bipolar cancellation” (BPC). The predominant BP nsEP parameters that induce BPC consist of a positive ...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703993/ https://www.ncbi.nlm.nih.gov/pubmed/29180756 http://dx.doi.org/10.1038/s41598-017-16142-6 |
_version_ | 1783281791404081152 |
---|---|
author | Valdez, Chris M. Barnes, Ronald A. Roth, Caleb C. Moen, Erick K. Throckmorton, Graham A. Ibey, Bennett L. |
author_facet | Valdez, Chris M. Barnes, Ronald A. Roth, Caleb C. Moen, Erick K. Throckmorton, Graham A. Ibey, Bennett L. |
author_sort | Valdez, Chris M. |
collection | PubMed |
description | A bipolar (BP) nanosecond electric pulse (nsEP) exposure generates reduced calcium influx compared to a unipolar (UP) nsEP. This attenuated physiological response from a BP nsEP exposure is termed “bipolar cancellation” (BPC). The predominant BP nsEP parameters that induce BPC consist of a positive polarity (↑) front pulse followed by the delivery of a negative polarity (↓) back pulse of equal voltage and width; thereby the duration is twice a UP nsEP exposure. We tested these BPC parameters, and discovered that a BP nsEP with symmetrical pulse widths is not required to generate BPC. For example, our data revealed the physiological response initiated by a ↑900 nsEP exposure can be cancelled by a second pulse that is a third of its duration. However, we observed a complete loss of BPC from a ↑300 nsEP followed by a ↓900 nsEP exposure. Spatiotemporal analysis revealed these asymmetrical BP nsEP exposures generate distinct local YO-PRO®-1 uptake patterns across the plasma membrane. From these findings, we generated a conceptual model that suggests BPC is a phenomenon balanced by localized charging and discharging events across the membrane. |
format | Online Article Text |
id | pubmed-5703993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57039932017-11-30 Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation Valdez, Chris M. Barnes, Ronald A. Roth, Caleb C. Moen, Erick K. Throckmorton, Graham A. Ibey, Bennett L. Sci Rep Article A bipolar (BP) nanosecond electric pulse (nsEP) exposure generates reduced calcium influx compared to a unipolar (UP) nsEP. This attenuated physiological response from a BP nsEP exposure is termed “bipolar cancellation” (BPC). The predominant BP nsEP parameters that induce BPC consist of a positive polarity (↑) front pulse followed by the delivery of a negative polarity (↓) back pulse of equal voltage and width; thereby the duration is twice a UP nsEP exposure. We tested these BPC parameters, and discovered that a BP nsEP with symmetrical pulse widths is not required to generate BPC. For example, our data revealed the physiological response initiated by a ↑900 nsEP exposure can be cancelled by a second pulse that is a third of its duration. However, we observed a complete loss of BPC from a ↑300 nsEP followed by a ↓900 nsEP exposure. Spatiotemporal analysis revealed these asymmetrical BP nsEP exposures generate distinct local YO-PRO®-1 uptake patterns across the plasma membrane. From these findings, we generated a conceptual model that suggests BPC is a phenomenon balanced by localized charging and discharging events across the membrane. Nature Publishing Group UK 2017-11-27 /pmc/articles/PMC5703993/ /pubmed/29180756 http://dx.doi.org/10.1038/s41598-017-16142-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Valdez, Chris M. Barnes, Ronald A. Roth, Caleb C. Moen, Erick K. Throckmorton, Graham A. Ibey, Bennett L. Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation |
title | Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation |
title_full | Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation |
title_fullStr | Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation |
title_full_unstemmed | Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation |
title_short | Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation |
title_sort | asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703993/ https://www.ncbi.nlm.nih.gov/pubmed/29180756 http://dx.doi.org/10.1038/s41598-017-16142-6 |
work_keys_str_mv | AT valdezchrism asymmetricalbipolarnanosecondelectricpulsewidthsmodifybipolarcancellation AT barnesronalda asymmetricalbipolarnanosecondelectricpulsewidthsmodifybipolarcancellation AT rothcalebc asymmetricalbipolarnanosecondelectricpulsewidthsmodifybipolarcancellation AT moenerickk asymmetricalbipolarnanosecondelectricpulsewidthsmodifybipolarcancellation AT throckmortongrahama asymmetricalbipolarnanosecondelectricpulsewidthsmodifybipolarcancellation AT ibeybennettl asymmetricalbipolarnanosecondelectricpulsewidthsmodifybipolarcancellation |