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Modified Langendorff technique for mouse heart cannulation: Improved heart quality and decreased risk of ischemia

Oscar Langendorff introduced the first method for isolating a heart with contractile activity in 1895. Since then, the Langendorff method has remained a powerful technique in cardiac research and has led to major advances in medicine. The primary goal of the Langendorff method is to provide an isola...

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Autor principal: Motayagheni, Negar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704101/
https://www.ncbi.nlm.nih.gov/pubmed/29204375
http://dx.doi.org/10.1016/j.mex.2017.11.004
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author Motayagheni, Negar
author_facet Motayagheni, Negar
author_sort Motayagheni, Negar
collection PubMed
description Oscar Langendorff introduced the first method for isolating a heart with contractile activity in 1895. Since then, the Langendorff method has remained a powerful technique in cardiac research and has led to major advances in medicine. The primary goal of the Langendorff method is to provide an isolated heart with oxygen and metabolites via a cannula inserted into the aorta. The Langendorff heart is a complex in vitro technique used primarily in pharmacological and physiological research that allows the evaluation of multiple cardiac hemodynamic parameters including, but not limited to, contractility and heart rate. This article will first provide a brief background of the Langendorff method as well as details regarding organ isolation. Finally, the article will discuss the benefits of a new technique for hanging the isolated heart aorta and the benefits of this technique over traditional methods
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spelling pubmed-57041012017-12-04 Modified Langendorff technique for mouse heart cannulation: Improved heart quality and decreased risk of ischemia Motayagheni, Negar MethodsX Pharmacology, Toxicology and Pharmaceutical Science Oscar Langendorff introduced the first method for isolating a heart with contractile activity in 1895. Since then, the Langendorff method has remained a powerful technique in cardiac research and has led to major advances in medicine. The primary goal of the Langendorff method is to provide an isolated heart with oxygen and metabolites via a cannula inserted into the aorta. The Langendorff heart is a complex in vitro technique used primarily in pharmacological and physiological research that allows the evaluation of multiple cardiac hemodynamic parameters including, but not limited to, contractility and heart rate. This article will first provide a brief background of the Langendorff method as well as details regarding organ isolation. Finally, the article will discuss the benefits of a new technique for hanging the isolated heart aorta and the benefits of this technique over traditional methods Elsevier 2017-11-14 /pmc/articles/PMC5704101/ /pubmed/29204375 http://dx.doi.org/10.1016/j.mex.2017.11.004 Text en © 2017 The Author http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Pharmacology, Toxicology and Pharmaceutical Science
Motayagheni, Negar
Modified Langendorff technique for mouse heart cannulation: Improved heart quality and decreased risk of ischemia
title Modified Langendorff technique for mouse heart cannulation: Improved heart quality and decreased risk of ischemia
title_full Modified Langendorff technique for mouse heart cannulation: Improved heart quality and decreased risk of ischemia
title_fullStr Modified Langendorff technique for mouse heart cannulation: Improved heart quality and decreased risk of ischemia
title_full_unstemmed Modified Langendorff technique for mouse heart cannulation: Improved heart quality and decreased risk of ischemia
title_short Modified Langendorff technique for mouse heart cannulation: Improved heart quality and decreased risk of ischemia
title_sort modified langendorff technique for mouse heart cannulation: improved heart quality and decreased risk of ischemia
topic Pharmacology, Toxicology and Pharmaceutical Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704101/
https://www.ncbi.nlm.nih.gov/pubmed/29204375
http://dx.doi.org/10.1016/j.mex.2017.11.004
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