Mapping DNA Methylation with High Throughput Nanopore Sequencing

Chemical modifications to DNA regulate its biological function. We present a framework for mapping methylation to cytosine and adenosine with the Oxford Nanopore Technologies MinION using its ionic current signal. We map three cytosine variants and two adenine variants. The results show that our mod...

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Detalles Bibliográficos
Autores principales: Rand, Arthur C., Jain, Miten, Eizenga, Jordan M., Musselman-Brown, Audrey, Olsen, Hugh E., Akeson, Mark, Paten, Benedict
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704956/
https://www.ncbi.nlm.nih.gov/pubmed/28218897
http://dx.doi.org/10.1038/nmeth.4189
Descripción
Sumario:Chemical modifications to DNA regulate its biological function. We present a framework for mapping methylation to cytosine and adenosine with the Oxford Nanopore Technologies MinION using its ionic current signal. We map three cytosine variants and two adenine variants. The results show that our model is sensitive enough to detect changes in genomic DNA methylation levels as a function of growth phase in E. coli.

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