Mapping DNA Methylation with High Throughput Nanopore Sequencing

Chemical modifications to DNA regulate its biological function. We present a framework for mapping methylation to cytosine and adenosine with the Oxford Nanopore Technologies MinION using its ionic current signal. We map three cytosine variants and two adenine variants. The results show that our mod...

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Detalles Bibliográficos
Autores principales: Rand, Arthur C., Jain, Miten, Eizenga, Jordan M., Musselman-Brown, Audrey, Olsen, Hugh E., Akeson, Mark, Paten, Benedict
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704956/
https://www.ncbi.nlm.nih.gov/pubmed/28218897
http://dx.doi.org/10.1038/nmeth.4189
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author Rand, Arthur C.
Jain, Miten
Eizenga, Jordan M.
Musselman-Brown, Audrey
Olsen, Hugh E.
Akeson, Mark
Paten, Benedict
author_facet Rand, Arthur C.
Jain, Miten
Eizenga, Jordan M.
Musselman-Brown, Audrey
Olsen, Hugh E.
Akeson, Mark
Paten, Benedict
author_sort Rand, Arthur C.
collection PubMed
description Chemical modifications to DNA regulate its biological function. We present a framework for mapping methylation to cytosine and adenosine with the Oxford Nanopore Technologies MinION using its ionic current signal. We map three cytosine variants and two adenine variants. The results show that our model is sensitive enough to detect changes in genomic DNA methylation levels as a function of growth phase in E. coli.
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spelling pubmed-57049562017-11-28 Mapping DNA Methylation with High Throughput Nanopore Sequencing Rand, Arthur C. Jain, Miten Eizenga, Jordan M. Musselman-Brown, Audrey Olsen, Hugh E. Akeson, Mark Paten, Benedict Nat Methods Article Chemical modifications to DNA regulate its biological function. We present a framework for mapping methylation to cytosine and adenosine with the Oxford Nanopore Technologies MinION using its ionic current signal. We map three cytosine variants and two adenine variants. The results show that our model is sensitive enough to detect changes in genomic DNA methylation levels as a function of growth phase in E. coli. 2017-02-20 2017-04 /pmc/articles/PMC5704956/ /pubmed/28218897 http://dx.doi.org/10.1038/nmeth.4189 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Rand, Arthur C.
Jain, Miten
Eizenga, Jordan M.
Musselman-Brown, Audrey
Olsen, Hugh E.
Akeson, Mark
Paten, Benedict
Mapping DNA Methylation with High Throughput Nanopore Sequencing
title Mapping DNA Methylation with High Throughput Nanopore Sequencing
title_full Mapping DNA Methylation with High Throughput Nanopore Sequencing
title_fullStr Mapping DNA Methylation with High Throughput Nanopore Sequencing
title_full_unstemmed Mapping DNA Methylation with High Throughput Nanopore Sequencing
title_short Mapping DNA Methylation with High Throughput Nanopore Sequencing
title_sort mapping dna methylation with high throughput nanopore sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704956/
https://www.ncbi.nlm.nih.gov/pubmed/28218897
http://dx.doi.org/10.1038/nmeth.4189
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