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Increased expression of Th17 cytokines and interleukin-22 correlates with disease activity in pristane-induced arthritis in rats
The objective of this study was to identify the key changed subtype of T helper cells (Th cells) and their cytokines in pristane-induced arthritis (PIA) in rats. The severity of arthritis was evaluated by body weight, clinical score, the perimeter of ankle and mid-paw and histological assessment of...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705111/ https://www.ncbi.nlm.nih.gov/pubmed/29182672 http://dx.doi.org/10.1371/journal.pone.0188199 |
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author | Wang, Bo Zhao, Panpan Zhou, Yan Meng, Liesu Zhu, Wenhua Jiang, Congshan Wang, Linyu Cai, Yongsong Lu, Shemin Hou, Weikun |
author_facet | Wang, Bo Zhao, Panpan Zhou, Yan Meng, Liesu Zhu, Wenhua Jiang, Congshan Wang, Linyu Cai, Yongsong Lu, Shemin Hou, Weikun |
author_sort | Wang, Bo |
collection | PubMed |
description | The objective of this study was to identify the key changed subtype of T helper cells (Th cells) and their cytokines in pristane-induced arthritis (PIA) in rats. The severity of arthritis was evaluated by body weight, clinical score, the perimeter of ankle and mid-paw and histological assessment of ankle joints. Cytokines of Th1, Th2 and Th17 were determined in the spleen and inguinal lymph nodes at 28 days after pristane injection by real-time qPCR. The mRNA levels of IL-22 receptors, IL-22R1 and IL-22BP, in the spleen were quantified by real-time qPCR. Additionally, IL-22 expression in synovial membrane was detected by Western blotting, and serum IL-22 concentration was determined by ELISA. Correlation between IL-22 concentration and clinical score was analyzed. By screening the cytokines of Th1, Th2 and Th17 expression profile, we found that the mRNA levels of Th17 cytokines were significantly increased in PIA rats. Particularly, a significant increase in the protein expression of IL-22 was determined in synovial membrane and serum from PIA rats, and correlated with clinical score. We conclude that IL-22 expression level was increased and correlated with disease severity, which indicated that IL-22 may play an important role in development of PIA, and was helpful to explorer the pathogenesis of rheumatoid arthritis. |
format | Online Article Text |
id | pubmed-5705111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57051112017-12-08 Increased expression of Th17 cytokines and interleukin-22 correlates with disease activity in pristane-induced arthritis in rats Wang, Bo Zhao, Panpan Zhou, Yan Meng, Liesu Zhu, Wenhua Jiang, Congshan Wang, Linyu Cai, Yongsong Lu, Shemin Hou, Weikun PLoS One Research Article The objective of this study was to identify the key changed subtype of T helper cells (Th cells) and their cytokines in pristane-induced arthritis (PIA) in rats. The severity of arthritis was evaluated by body weight, clinical score, the perimeter of ankle and mid-paw and histological assessment of ankle joints. Cytokines of Th1, Th2 and Th17 were determined in the spleen and inguinal lymph nodes at 28 days after pristane injection by real-time qPCR. The mRNA levels of IL-22 receptors, IL-22R1 and IL-22BP, in the spleen were quantified by real-time qPCR. Additionally, IL-22 expression in synovial membrane was detected by Western blotting, and serum IL-22 concentration was determined by ELISA. Correlation between IL-22 concentration and clinical score was analyzed. By screening the cytokines of Th1, Th2 and Th17 expression profile, we found that the mRNA levels of Th17 cytokines were significantly increased in PIA rats. Particularly, a significant increase in the protein expression of IL-22 was determined in synovial membrane and serum from PIA rats, and correlated with clinical score. We conclude that IL-22 expression level was increased and correlated with disease severity, which indicated that IL-22 may play an important role in development of PIA, and was helpful to explorer the pathogenesis of rheumatoid arthritis. Public Library of Science 2017-11-28 /pmc/articles/PMC5705111/ /pubmed/29182672 http://dx.doi.org/10.1371/journal.pone.0188199 Text en © 2017 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wang, Bo Zhao, Panpan Zhou, Yan Meng, Liesu Zhu, Wenhua Jiang, Congshan Wang, Linyu Cai, Yongsong Lu, Shemin Hou, Weikun Increased expression of Th17 cytokines and interleukin-22 correlates with disease activity in pristane-induced arthritis in rats |
title | Increased expression of Th17 cytokines and interleukin-22 correlates with disease activity in pristane-induced arthritis in rats |
title_full | Increased expression of Th17 cytokines and interleukin-22 correlates with disease activity in pristane-induced arthritis in rats |
title_fullStr | Increased expression of Th17 cytokines and interleukin-22 correlates with disease activity in pristane-induced arthritis in rats |
title_full_unstemmed | Increased expression of Th17 cytokines and interleukin-22 correlates with disease activity in pristane-induced arthritis in rats |
title_short | Increased expression of Th17 cytokines and interleukin-22 correlates with disease activity in pristane-induced arthritis in rats |
title_sort | increased expression of th17 cytokines and interleukin-22 correlates with disease activity in pristane-induced arthritis in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705111/ https://www.ncbi.nlm.nih.gov/pubmed/29182672 http://dx.doi.org/10.1371/journal.pone.0188199 |
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