Virus-host protein-protein interactions of mycobacteriophage Giles

Mycobacteriophage are viruses that infect mycobacteria. More than 1,400 mycobacteriophage genomes have been sequenced, coding for over one hundred thousand proteins of unknown functions. Here we investigate mycobacteriophage Giles-host protein-protein interactions (PPIs) using yeast two-hybrid scree...

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Autores principales: Mehla, Jitender, Dedrick, Rebekah M., Caufield, J. Harry, Wagemans, Jeroen, Sakhawalkar, Neha, Johnson, Allison, Hatfull, Graham F., Uetz, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705681/
https://www.ncbi.nlm.nih.gov/pubmed/29184079
http://dx.doi.org/10.1038/s41598-017-16303-7
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author Mehla, Jitender
Dedrick, Rebekah M.
Caufield, J. Harry
Wagemans, Jeroen
Sakhawalkar, Neha
Johnson, Allison
Hatfull, Graham F.
Uetz, Peter
author_facet Mehla, Jitender
Dedrick, Rebekah M.
Caufield, J. Harry
Wagemans, Jeroen
Sakhawalkar, Neha
Johnson, Allison
Hatfull, Graham F.
Uetz, Peter
author_sort Mehla, Jitender
collection PubMed
description Mycobacteriophage are viruses that infect mycobacteria. More than 1,400 mycobacteriophage genomes have been sequenced, coding for over one hundred thousand proteins of unknown functions. Here we investigate mycobacteriophage Giles-host protein-protein interactions (PPIs) using yeast two-hybrid screening (Y2H). A total of 25 reproducible PPIs were found for a selected set of 10 Giles proteins, including a putative virion assembly protein (gp17), the phage integrase (gp29), the endolysin (gp31), the phage repressor (gp47), and six proteins of unknown function (gp34, gp35, gp54, gp56, gp64, and gp65). We note that overexpression of the proteins is toxic to M. smegmatis, although whether this toxicity and the associated changes in cellular morphology are related to the putative interactions revealed in the Y2H screen is unclear.
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spelling pubmed-57056812017-12-05 Virus-host protein-protein interactions of mycobacteriophage Giles Mehla, Jitender Dedrick, Rebekah M. Caufield, J. Harry Wagemans, Jeroen Sakhawalkar, Neha Johnson, Allison Hatfull, Graham F. Uetz, Peter Sci Rep Article Mycobacteriophage are viruses that infect mycobacteria. More than 1,400 mycobacteriophage genomes have been sequenced, coding for over one hundred thousand proteins of unknown functions. Here we investigate mycobacteriophage Giles-host protein-protein interactions (PPIs) using yeast two-hybrid screening (Y2H). A total of 25 reproducible PPIs were found for a selected set of 10 Giles proteins, including a putative virion assembly protein (gp17), the phage integrase (gp29), the endolysin (gp31), the phage repressor (gp47), and six proteins of unknown function (gp34, gp35, gp54, gp56, gp64, and gp65). We note that overexpression of the proteins is toxic to M. smegmatis, although whether this toxicity and the associated changes in cellular morphology are related to the putative interactions revealed in the Y2H screen is unclear. Nature Publishing Group UK 2017-11-28 /pmc/articles/PMC5705681/ /pubmed/29184079 http://dx.doi.org/10.1038/s41598-017-16303-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mehla, Jitender
Dedrick, Rebekah M.
Caufield, J. Harry
Wagemans, Jeroen
Sakhawalkar, Neha
Johnson, Allison
Hatfull, Graham F.
Uetz, Peter
Virus-host protein-protein interactions of mycobacteriophage Giles
title Virus-host protein-protein interactions of mycobacteriophage Giles
title_full Virus-host protein-protein interactions of mycobacteriophage Giles
title_fullStr Virus-host protein-protein interactions of mycobacteriophage Giles
title_full_unstemmed Virus-host protein-protein interactions of mycobacteriophage Giles
title_short Virus-host protein-protein interactions of mycobacteriophage Giles
title_sort virus-host protein-protein interactions of mycobacteriophage giles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705681/
https://www.ncbi.nlm.nih.gov/pubmed/29184079
http://dx.doi.org/10.1038/s41598-017-16303-7
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