Immobilized aptamer on gold electrode senses trace amount of aflatoxin M1

An electrochemical aptasensor for detection of trace amounts of aflatoxin M1 was developed. This required immobilization of aptamer on screen printed gold electrode comprising of working electrode, counter electrode and reference electrode and was achieved by sequentially layering dithiodipropionic...

Descripción completa

Detalles Bibliográficos
Autores principales: Pandey, Amit Kumar, Rajput, Yudhishthir Singh, Sharma, Rajan, Singh, Dheer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705768/
https://www.ncbi.nlm.nih.gov/pubmed/29214120
http://dx.doi.org/10.1007/s13204-017-0629-0
_version_ 1783282091013701632
author Pandey, Amit Kumar
Rajput, Yudhishthir Singh
Sharma, Rajan
Singh, Dheer
author_facet Pandey, Amit Kumar
Rajput, Yudhishthir Singh
Sharma, Rajan
Singh, Dheer
author_sort Pandey, Amit Kumar
collection PubMed
description An electrochemical aptasensor for detection of trace amounts of aflatoxin M1 was developed. This required immobilization of aptamer on screen printed gold electrode comprising of working electrode, counter electrode and reference electrode and was achieved by sequentially layering dithiodipropionic acid, streptavidin and biotinylated-tetraethylene glycol-aptamer. Immobilization of aptamer was monitored by cyclic voltammetry. Peak current in square wave voltammogram was inversely related to logarithmic concentration of aflatoxin M1. Dynamic range of sensor was 1–10(5) ppt aflatoxin M1. Sensor can be regenerated by treating electrode with 10% sodium dodecyl sulfate or 40 mM tris-HCl (pH 8.0) containing 10 mM ethylenediaminetetraacetic acid and 0.02% tween-20.
format Online
Article
Text
id pubmed-5705768
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-57057682017-12-04 Immobilized aptamer on gold electrode senses trace amount of aflatoxin M1 Pandey, Amit Kumar Rajput, Yudhishthir Singh Sharma, Rajan Singh, Dheer Appl Nanosci Original Article An electrochemical aptasensor for detection of trace amounts of aflatoxin M1 was developed. This required immobilization of aptamer on screen printed gold electrode comprising of working electrode, counter electrode and reference electrode and was achieved by sequentially layering dithiodipropionic acid, streptavidin and biotinylated-tetraethylene glycol-aptamer. Immobilization of aptamer was monitored by cyclic voltammetry. Peak current in square wave voltammogram was inversely related to logarithmic concentration of aflatoxin M1. Dynamic range of sensor was 1–10(5) ppt aflatoxin M1. Sensor can be regenerated by treating electrode with 10% sodium dodecyl sulfate or 40 mM tris-HCl (pH 8.0) containing 10 mM ethylenediaminetetraacetic acid and 0.02% tween-20. Springer Berlin Heidelberg 2017-11-13 2017 /pmc/articles/PMC5705768/ /pubmed/29214120 http://dx.doi.org/10.1007/s13204-017-0629-0 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Pandey, Amit Kumar
Rajput, Yudhishthir Singh
Sharma, Rajan
Singh, Dheer
Immobilized aptamer on gold electrode senses trace amount of aflatoxin M1
title Immobilized aptamer on gold electrode senses trace amount of aflatoxin M1
title_full Immobilized aptamer on gold electrode senses trace amount of aflatoxin M1
title_fullStr Immobilized aptamer on gold electrode senses trace amount of aflatoxin M1
title_full_unstemmed Immobilized aptamer on gold electrode senses trace amount of aflatoxin M1
title_short Immobilized aptamer on gold electrode senses trace amount of aflatoxin M1
title_sort immobilized aptamer on gold electrode senses trace amount of aflatoxin m1
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705768/
https://www.ncbi.nlm.nih.gov/pubmed/29214120
http://dx.doi.org/10.1007/s13204-017-0629-0
work_keys_str_mv AT pandeyamitkumar immobilizedaptamerongoldelectrodesensestraceamountofaflatoxinm1
AT rajputyudhishthirsingh immobilizedaptamerongoldelectrodesensestraceamountofaflatoxinm1
AT sharmarajan immobilizedaptamerongoldelectrodesensestraceamountofaflatoxinm1
AT singhdheer immobilizedaptamerongoldelectrodesensestraceamountofaflatoxinm1

Ejemplares similares