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FAM13A as a Novel Hypoxia-Induced Gene in Non-Small Cell Lung Cancer

Several genome-wide association studies (GWASs), have identified that FAM13A and IREB2 loci are associated with lung cancer, but the mechanisms by which these genes contribute to lung diseases susceptibility, especially in hypoxia context, are unknown. Hypoxia has been identified as a major negative...

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Autores principales: Ziółkowska-Suchanek, Iwona, Mosor, Maria, Podralska, Marta, Iżykowska, Katarzyna, Gabryel, Piotr, Dyszkiewicz, Wojciech, Słomski, Ryszard, Nowak, Jerzy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705994/
https://www.ncbi.nlm.nih.gov/pubmed/29187867
http://dx.doi.org/10.7150/jca.20342
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author Ziółkowska-Suchanek, Iwona
Mosor, Maria
Podralska, Marta
Iżykowska, Katarzyna
Gabryel, Piotr
Dyszkiewicz, Wojciech
Słomski, Ryszard
Nowak, Jerzy
author_facet Ziółkowska-Suchanek, Iwona
Mosor, Maria
Podralska, Marta
Iżykowska, Katarzyna
Gabryel, Piotr
Dyszkiewicz, Wojciech
Słomski, Ryszard
Nowak, Jerzy
author_sort Ziółkowska-Suchanek, Iwona
collection PubMed
description Several genome-wide association studies (GWASs), have identified that FAM13A and IREB2 loci are associated with lung cancer, but the mechanisms by which these genes contribute to lung diseases susceptibility, especially in hypoxia context, are unknown. Hypoxia has been identified as a major negative factor for tumor progression in clinical observation. It has been suggested, that lower oxygen tension, may modulate the IREB2 and FAM13A activity. However, the role of these genes in hypoxia response has not been explained. To precise the role of these genes in hypoxia response, we analyzed the FAM13A and IREB2 expression, in lung cancer cells in vitro and lung cancer tissue fragments cultured ex vivo. Three cell lines: non-small cell lung cancer (A549, CORL-105), human lung fibroblasts (HL) and 37 lung cancer tissue fragments were analyzed. The expression of IREB2, FAM13A and HIF1α after sustained 72 hours of hypoxia versus normal oxygen concentration were analyzed by TaqMan® Gene Expression Assays and Western Blot. The expression of FAM13A was significantly up-regulated by hypoxia in two lung cancer cell lines (A549, CORL-105, P<0.001), both at the level of protein and mRNA, and in lung cancer tissue fragments (P=0.0004). The IREB2 was down-regulated after hypoxia in A549 cancer cells (P<0.001). Conclusions: We found that FAM13A overexpression in human lung cancer cell lines overlapped with hypoxia effect on lung cancer tissues. FAM13A is strongly induced by hypoxia and may be identified as a novel hypoxia-induced gene in non-small cell lung cancer.
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spelling pubmed-57059942017-11-29 FAM13A as a Novel Hypoxia-Induced Gene in Non-Small Cell Lung Cancer Ziółkowska-Suchanek, Iwona Mosor, Maria Podralska, Marta Iżykowska, Katarzyna Gabryel, Piotr Dyszkiewicz, Wojciech Słomski, Ryszard Nowak, Jerzy J Cancer Short Research Communication Several genome-wide association studies (GWASs), have identified that FAM13A and IREB2 loci are associated with lung cancer, but the mechanisms by which these genes contribute to lung diseases susceptibility, especially in hypoxia context, are unknown. Hypoxia has been identified as a major negative factor for tumor progression in clinical observation. It has been suggested, that lower oxygen tension, may modulate the IREB2 and FAM13A activity. However, the role of these genes in hypoxia response has not been explained. To precise the role of these genes in hypoxia response, we analyzed the FAM13A and IREB2 expression, in lung cancer cells in vitro and lung cancer tissue fragments cultured ex vivo. Three cell lines: non-small cell lung cancer (A549, CORL-105), human lung fibroblasts (HL) and 37 lung cancer tissue fragments were analyzed. The expression of IREB2, FAM13A and HIF1α after sustained 72 hours of hypoxia versus normal oxygen concentration were analyzed by TaqMan® Gene Expression Assays and Western Blot. The expression of FAM13A was significantly up-regulated by hypoxia in two lung cancer cell lines (A549, CORL-105, P<0.001), both at the level of protein and mRNA, and in lung cancer tissue fragments (P=0.0004). The IREB2 was down-regulated after hypoxia in A549 cancer cells (P<0.001). Conclusions: We found that FAM13A overexpression in human lung cancer cell lines overlapped with hypoxia effect on lung cancer tissues. FAM13A is strongly induced by hypoxia and may be identified as a novel hypoxia-induced gene in non-small cell lung cancer. Ivyspring International Publisher 2017-10-23 /pmc/articles/PMC5705994/ /pubmed/29187867 http://dx.doi.org/10.7150/jca.20342 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Short Research Communication
Ziółkowska-Suchanek, Iwona
Mosor, Maria
Podralska, Marta
Iżykowska, Katarzyna
Gabryel, Piotr
Dyszkiewicz, Wojciech
Słomski, Ryszard
Nowak, Jerzy
FAM13A as a Novel Hypoxia-Induced Gene in Non-Small Cell Lung Cancer
title FAM13A as a Novel Hypoxia-Induced Gene in Non-Small Cell Lung Cancer
title_full FAM13A as a Novel Hypoxia-Induced Gene in Non-Small Cell Lung Cancer
title_fullStr FAM13A as a Novel Hypoxia-Induced Gene in Non-Small Cell Lung Cancer
title_full_unstemmed FAM13A as a Novel Hypoxia-Induced Gene in Non-Small Cell Lung Cancer
title_short FAM13A as a Novel Hypoxia-Induced Gene in Non-Small Cell Lung Cancer
title_sort fam13a as a novel hypoxia-induced gene in non-small cell lung cancer
topic Short Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705994/
https://www.ncbi.nlm.nih.gov/pubmed/29187867
http://dx.doi.org/10.7150/jca.20342
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