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Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression
Long non-coding RNAs (lncRNAs) are involved in the pathology of various tumors, including colorectal cancer (CRC). However, the role of lncRNA in CRC liver metastasis remains unclear. Methods: a microarray was performed to identify the differentially expressed lncRNAs between CRC tissues with and wi...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706103/ https://www.ncbi.nlm.nih.gov/pubmed/29187907 http://dx.doi.org/10.7150/thno.20942 |
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author | Chen, Dong-liang Lu, Yun-xin Zhang, Jia-xing Wei, Xiao-li Wang, Feng Zeng, Zhao-lei Pan, Zhi-zhong Yuan, Yun-fei Wang, Feng-hua Pelicano, Helene Chiao, Paul J. Huang, Peng Xie, Dan Li, Yu-hong Ju, Huai-qiang Xu, Rui-hua |
author_facet | Chen, Dong-liang Lu, Yun-xin Zhang, Jia-xing Wei, Xiao-li Wang, Feng Zeng, Zhao-lei Pan, Zhi-zhong Yuan, Yun-fei Wang, Feng-hua Pelicano, Helene Chiao, Paul J. Huang, Peng Xie, Dan Li, Yu-hong Ju, Huai-qiang Xu, Rui-hua |
author_sort | Chen, Dong-liang |
collection | PubMed |
description | Long non-coding RNAs (lncRNAs) are involved in the pathology of various tumors, including colorectal cancer (CRC). However, the role of lncRNA in CRC liver metastasis remains unclear. Methods: a microarray was performed to identify the differentially expressed lncRNAs between CRC tissues with and without liver metastasis. Survival analysis was evaluated using the Kaplan-Meier method and assessed using the log-rank test. In vitro and in vivo assays were preformed to explore the biological effects of the differentially expressed lncRNA in CRC cells. Results: the lncRNA UICLM (up-regulated in colorectal cancer liver metastasis) was significantly up-regulated in cases of CRC with liver metastasis. Moreover, UICLM expression was higher in CRC tissues than in normal tissues, and UICLM expression was associated with poor patient survival. Knockdown of UICLM inhibited CRC cell proliferation, invasion, epithelial-mesenchymal transition (EMT) and CRC stem cell formation in vitro as well as tumor growth and liver metastasis in vivo. Ectopic expression of UICLM promoted CRC cell proliferation and invasion. Mechanistic investigations revealed that UICLM induced its biological effects by regulating ZEB2, as the oncogenesis facilitated by UICLM was inhibited by ZEB2 depletion. Further study indicated that UICLM acted as a competing endogenous RNA (ceRNA) for miR-215 to regulate ZEB2 expression. Conclusions: taken together, our findings demonstrate how UICLM induces CRC liver metastasis and may offer a novel prognostic marker and therapeutic target for this disease. |
format | Online Article Text |
id | pubmed-5706103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-57061032017-11-29 Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression Chen, Dong-liang Lu, Yun-xin Zhang, Jia-xing Wei, Xiao-li Wang, Feng Zeng, Zhao-lei Pan, Zhi-zhong Yuan, Yun-fei Wang, Feng-hua Pelicano, Helene Chiao, Paul J. Huang, Peng Xie, Dan Li, Yu-hong Ju, Huai-qiang Xu, Rui-hua Theranostics Research Paper Long non-coding RNAs (lncRNAs) are involved in the pathology of various tumors, including colorectal cancer (CRC). However, the role of lncRNA in CRC liver metastasis remains unclear. Methods: a microarray was performed to identify the differentially expressed lncRNAs between CRC tissues with and without liver metastasis. Survival analysis was evaluated using the Kaplan-Meier method and assessed using the log-rank test. In vitro and in vivo assays were preformed to explore the biological effects of the differentially expressed lncRNA in CRC cells. Results: the lncRNA UICLM (up-regulated in colorectal cancer liver metastasis) was significantly up-regulated in cases of CRC with liver metastasis. Moreover, UICLM expression was higher in CRC tissues than in normal tissues, and UICLM expression was associated with poor patient survival. Knockdown of UICLM inhibited CRC cell proliferation, invasion, epithelial-mesenchymal transition (EMT) and CRC stem cell formation in vitro as well as tumor growth and liver metastasis in vivo. Ectopic expression of UICLM promoted CRC cell proliferation and invasion. Mechanistic investigations revealed that UICLM induced its biological effects by regulating ZEB2, as the oncogenesis facilitated by UICLM was inhibited by ZEB2 depletion. Further study indicated that UICLM acted as a competing endogenous RNA (ceRNA) for miR-215 to regulate ZEB2 expression. Conclusions: taken together, our findings demonstrate how UICLM induces CRC liver metastasis and may offer a novel prognostic marker and therapeutic target for this disease. Ivyspring International Publisher 2017-10-17 /pmc/articles/PMC5706103/ /pubmed/29187907 http://dx.doi.org/10.7150/thno.20942 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Chen, Dong-liang Lu, Yun-xin Zhang, Jia-xing Wei, Xiao-li Wang, Feng Zeng, Zhao-lei Pan, Zhi-zhong Yuan, Yun-fei Wang, Feng-hua Pelicano, Helene Chiao, Paul J. Huang, Peng Xie, Dan Li, Yu-hong Ju, Huai-qiang Xu, Rui-hua Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression |
title | Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression |
title_full | Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression |
title_fullStr | Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression |
title_full_unstemmed | Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression |
title_short | Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression |
title_sort | long non-coding rna uiclm promotes colorectal cancer liver metastasis by acting as a cerna for microrna-215 to regulate zeb2 expression |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706103/ https://www.ncbi.nlm.nih.gov/pubmed/29187907 http://dx.doi.org/10.7150/thno.20942 |
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