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Cell-cycle-specific Cellular Responses to Sonoporation
Microbubble-mediated sonoporation has shown its great potential in facilitating intracellular uptake of gene/drugs and other therapeutic agents that are otherwise difficult to enter cells. However, the biophysical mechanisms underlying microbubble-cell interactions remain unclear. Particularly, it i...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706108/ https://www.ncbi.nlm.nih.gov/pubmed/29187912 http://dx.doi.org/10.7150/thno.20820 |
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author | Fan, Pengfei Zhang, Yi Guo, Xiasheng Cai, Chenliang Wang, Maochen Yang, Dongxin Li, Yiran Tu, Juan Crum, Lawrence A. Wu, Junru Zhang, Dong |
author_facet | Fan, Pengfei Zhang, Yi Guo, Xiasheng Cai, Chenliang Wang, Maochen Yang, Dongxin Li, Yiran Tu, Juan Crum, Lawrence A. Wu, Junru Zhang, Dong |
author_sort | Fan, Pengfei |
collection | PubMed |
description | Microbubble-mediated sonoporation has shown its great potential in facilitating intracellular uptake of gene/drugs and other therapeutic agents that are otherwise difficult to enter cells. However, the biophysical mechanisms underlying microbubble-cell interactions remain unclear. Particularly, it is still a major challenge to get a comprehensive understanding of the impact of cell cycle phase on the cellular responses simultaneously occurring in cell membrane and cytoskeleton induced by microbubble sonoporation. Methods: Here, efficient synchronizations were performed to arrest human cervical epithelial carcinoma (HeLa) cells in individual cycle phases. The, topography and stiffness of synchronized cells were examined using atomic force microscopy. The variations in cell membrane permeabilization and cytoskeleton arrangement induced by sonoporation were analyzed simultaneously by a real-time fluorescence imaging system. Results: The results showed that G1-phase cells typically had the largest height and elastic modulus, while S-phase cells were generally the flattest and softest ones. Consequently, the S-Phase was found to be the preferred cycle for instantaneous sonoporation treatment, due to the greatest enhancement of membrane permeability and the fastest cytoskeleton disassembly at the early stage after sonoporation. Conclusion: The current findings may benefit ongoing efforts aiming to pursue rational utilization of microbubble-mediated sonoporation in cell cycle-targeted gene/drug delivery for cancer therapy. |
format | Online Article Text |
id | pubmed-5706108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-57061082017-11-29 Cell-cycle-specific Cellular Responses to Sonoporation Fan, Pengfei Zhang, Yi Guo, Xiasheng Cai, Chenliang Wang, Maochen Yang, Dongxin Li, Yiran Tu, Juan Crum, Lawrence A. Wu, Junru Zhang, Dong Theranostics Research Paper Microbubble-mediated sonoporation has shown its great potential in facilitating intracellular uptake of gene/drugs and other therapeutic agents that are otherwise difficult to enter cells. However, the biophysical mechanisms underlying microbubble-cell interactions remain unclear. Particularly, it is still a major challenge to get a comprehensive understanding of the impact of cell cycle phase on the cellular responses simultaneously occurring in cell membrane and cytoskeleton induced by microbubble sonoporation. Methods: Here, efficient synchronizations were performed to arrest human cervical epithelial carcinoma (HeLa) cells in individual cycle phases. The, topography and stiffness of synchronized cells were examined using atomic force microscopy. The variations in cell membrane permeabilization and cytoskeleton arrangement induced by sonoporation were analyzed simultaneously by a real-time fluorescence imaging system. Results: The results showed that G1-phase cells typically had the largest height and elastic modulus, while S-phase cells were generally the flattest and softest ones. Consequently, the S-Phase was found to be the preferred cycle for instantaneous sonoporation treatment, due to the greatest enhancement of membrane permeability and the fastest cytoskeleton disassembly at the early stage after sonoporation. Conclusion: The current findings may benefit ongoing efforts aiming to pursue rational utilization of microbubble-mediated sonoporation in cell cycle-targeted gene/drug delivery for cancer therapy. Ivyspring International Publisher 2017-11-03 /pmc/articles/PMC5706108/ /pubmed/29187912 http://dx.doi.org/10.7150/thno.20820 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Fan, Pengfei Zhang, Yi Guo, Xiasheng Cai, Chenliang Wang, Maochen Yang, Dongxin Li, Yiran Tu, Juan Crum, Lawrence A. Wu, Junru Zhang, Dong Cell-cycle-specific Cellular Responses to Sonoporation |
title | Cell-cycle-specific Cellular Responses to Sonoporation |
title_full | Cell-cycle-specific Cellular Responses to Sonoporation |
title_fullStr | Cell-cycle-specific Cellular Responses to Sonoporation |
title_full_unstemmed | Cell-cycle-specific Cellular Responses to Sonoporation |
title_short | Cell-cycle-specific Cellular Responses to Sonoporation |
title_sort | cell-cycle-specific cellular responses to sonoporation |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706108/ https://www.ncbi.nlm.nih.gov/pubmed/29187912 http://dx.doi.org/10.7150/thno.20820 |
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