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Fluorescent Magnetopolymersomes: A Theranostic Platform to Track Intracellular Delivery
We present a potential theranostic delivery platform based on the amphiphilic diblock copolymer polybutadiene-block-poly (ethylene oxide) combining covalent fluorescent labeling and membrane incorporation of superparamagnetic iron oxide nanoparticles for multimodal imaging. A simple self-assembly an...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706250/ https://www.ncbi.nlm.nih.gov/pubmed/29137172 http://dx.doi.org/10.3390/ma10111303 |
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author | Bixner, Oliver Gal, Noga Zaba, Christoph Scheberl, Andrea Reimhult, Erik |
author_facet | Bixner, Oliver Gal, Noga Zaba, Christoph Scheberl, Andrea Reimhult, Erik |
author_sort | Bixner, Oliver |
collection | PubMed |
description | We present a potential theranostic delivery platform based on the amphiphilic diblock copolymer polybutadiene-block-poly (ethylene oxide) combining covalent fluorescent labeling and membrane incorporation of superparamagnetic iron oxide nanoparticles for multimodal imaging. A simple self-assembly and labeling approach to create the fluorescent and magnetic vesicles is described. Cell uptake of the densely PEGylated polymer vesicles could be altered by surface modifications that vary surface charge and accessibility of the membrane active species. Cell uptake and cytotoxicity were evaluated by confocal microscopy, transmission electron microscopy, iron content and metabolic assays, utilizing multimodal tracking of membrane fluorophores and nanoparticles. Cationic functionalization of vesicles promoted endocytotic uptake. In particular, incorporation of cationic lipids in the polymersome membrane yielded tremendously increased uptake of polymersomes and magnetopolymersomes without increase in cytotoxicity. Ultrastructure investigations showed that cationic magnetopolymersomes disintegrated upon hydrolysis, including the dissolution of incorporated iron oxide nanoparticles. The presented platform could find future use in theranostic multimodal imaging in vivo and magnetically triggered delivery by incorporation of thermorepsonsive amphiphiles that can break the membrane integrity upon magnetic heating via the embedded superparamagnetic nanoparticles. |
format | Online Article Text |
id | pubmed-5706250 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57062502017-12-04 Fluorescent Magnetopolymersomes: A Theranostic Platform to Track Intracellular Delivery Bixner, Oliver Gal, Noga Zaba, Christoph Scheberl, Andrea Reimhult, Erik Materials (Basel) Article We present a potential theranostic delivery platform based on the amphiphilic diblock copolymer polybutadiene-block-poly (ethylene oxide) combining covalent fluorescent labeling and membrane incorporation of superparamagnetic iron oxide nanoparticles for multimodal imaging. A simple self-assembly and labeling approach to create the fluorescent and magnetic vesicles is described. Cell uptake of the densely PEGylated polymer vesicles could be altered by surface modifications that vary surface charge and accessibility of the membrane active species. Cell uptake and cytotoxicity were evaluated by confocal microscopy, transmission electron microscopy, iron content and metabolic assays, utilizing multimodal tracking of membrane fluorophores and nanoparticles. Cationic functionalization of vesicles promoted endocytotic uptake. In particular, incorporation of cationic lipids in the polymersome membrane yielded tremendously increased uptake of polymersomes and magnetopolymersomes without increase in cytotoxicity. Ultrastructure investigations showed that cationic magnetopolymersomes disintegrated upon hydrolysis, including the dissolution of incorporated iron oxide nanoparticles. The presented platform could find future use in theranostic multimodal imaging in vivo and magnetically triggered delivery by incorporation of thermorepsonsive amphiphiles that can break the membrane integrity upon magnetic heating via the embedded superparamagnetic nanoparticles. MDPI 2017-11-13 /pmc/articles/PMC5706250/ /pubmed/29137172 http://dx.doi.org/10.3390/ma10111303 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bixner, Oliver Gal, Noga Zaba, Christoph Scheberl, Andrea Reimhult, Erik Fluorescent Magnetopolymersomes: A Theranostic Platform to Track Intracellular Delivery |
title | Fluorescent Magnetopolymersomes: A Theranostic Platform to Track Intracellular Delivery |
title_full | Fluorescent Magnetopolymersomes: A Theranostic Platform to Track Intracellular Delivery |
title_fullStr | Fluorescent Magnetopolymersomes: A Theranostic Platform to Track Intracellular Delivery |
title_full_unstemmed | Fluorescent Magnetopolymersomes: A Theranostic Platform to Track Intracellular Delivery |
title_short | Fluorescent Magnetopolymersomes: A Theranostic Platform to Track Intracellular Delivery |
title_sort | fluorescent magnetopolymersomes: a theranostic platform to track intracellular delivery |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706250/ https://www.ncbi.nlm.nih.gov/pubmed/29137172 http://dx.doi.org/10.3390/ma10111303 |
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