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Cytocompatibility of a self-adhesive gutta-percha root-filling material

CONTEXT: A novel root-filling material based on the incorporation of ultrafine alkaline bioactive glass particles (bioactive gutta-percha, [BGP]) was developed to work without sealer. AIM: In the present study, the objective was to verify the in vitro biological response to this material by assessin...

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Detalles Bibliográficos
Autores principales: Nascimento, Joyce, Scelza, Miriam Zaccaro, Alves, Gutemberg Gomes, Linhares, Adriana, Canabarro, Antonio, Granjeiro, Jose Mauro, De-Deus, Gustavo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706313/
https://www.ncbi.nlm.nih.gov/pubmed/29279616
http://dx.doi.org/10.4103/0972-0707.218303
Descripción
Sumario:CONTEXT: A novel root-filling material based on the incorporation of ultrafine alkaline bioactive glass particles (bioactive gutta-percha, [BGP]) was developed to work without sealer. AIM: In the present study, the objective was to verify the in vitro biological response to this material by assessing its cytocompatibility. MATERIALS AND METHODS: Prototypes of BGP were compared to conventional gutta-percha (GP), dense polystyrene beads as a negative control and fragments of latex as a positive control. Extracts of each material were prepared according to ISO 10993-5:2009, and human osteoblast-like cells in primary culture were exposed to all extracts for 24 h. Cell viability was assayed sequentially for three different parameters: mitochondrial activity, membrane integrity, and cell density. STATISTICAL ANALYSIS USED: Nonparametric analysis (using Kruskal–Wallis test combined with post hoc Dunn's test) was performed for comparison among groups, with significance established at 5%. RESULTS: BGP reduced mitochondrial activity to 62% of control, but presented no toxicity on membrane integrity and proliferation assays. BGP effect on metabolism was dose-dependent and reduced to acceptable levels with dilution. CONCLUSION: The novel GP material presented slight dose-dependent effects on cell metabolism but did not affect cell survival.