CD11b promotes the differentiation of osteoclasts induced by RANKL through the spleen tyrosine kinase signalling pathway

Macrophage surface antigen‐1 (Mac‐1, CD11b/CD18) has been implicated in the regulation of osteoclastogenesis. In the synovial tissues of patients with aseptic loosening after total hip replacement, CD11b was up‐regulated, which indicated that CD11b is closely involved in osteolysis around the prosthesis. We found that CD11b, but not CD18, promoted osteoclast (OC) maturation. Here, we show CD11b up‐regulated the levels of spleen tyrosine kinase (Syk), c‐Fos and nuclear factor of activated T cells, cytoplasmic‐1 (NFATc1), as well as the activity of extracellular‐regulated kinase (Erk), and as a result, osteoclast precursors (OCPs) differentiated and became tartrate‐resistant acid phosphatase (TRAP)‐positive. In addition, increased tumour necrosis factor‐α (TNF‐α) induced by ultra‐high molecular weight polyethylene (UHMWPE) particles up‐regulated the level of CD11b. Taken together, these findings suggest that CD11b is a positive regulator of osteoclastogenesis and that it functions by activating the Syk signalling pathway, while CD18 does not have the same effect.