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Age-related modulation of plasmatic beta-Galactosidase activity in healthy subjects and in patients affected by T2DM

β-Galactosidase (β-Gal) activity has been the most extensively utilized biomarker for the detection of cellular senescence. It can be measured also in plasma, and few recent evidence showed an altered plasmatic β-Gal activity in patients affected by some age-related diseases (ARDs). Since T2DM is on...

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Autores principales: Spazzafumo, Liana, Mensà, Emanuela, Matacchione, Giulia, Galeazzi, Tiziana, Zampini, Lucia, Recchioni, Rina, Marcheselli, Fiorella, Prattichizzo, Francesco, Testa, Roberto, Antonicelli, Roberto, Garagnani, Paolo, Boemi, Massimo, Bonafè, Massimiliano, Bonfigli, Anna Rita, Procopio, Antonio Domenico, Olivieri, Fabiola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706799/
https://www.ncbi.nlm.nih.gov/pubmed/29212153
http://dx.doi.org/10.18632/oncotarget.21848
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author Spazzafumo, Liana
Mensà, Emanuela
Matacchione, Giulia
Galeazzi, Tiziana
Zampini, Lucia
Recchioni, Rina
Marcheselli, Fiorella
Prattichizzo, Francesco
Testa, Roberto
Antonicelli, Roberto
Garagnani, Paolo
Boemi, Massimo
Bonafè, Massimiliano
Bonfigli, Anna Rita
Procopio, Antonio Domenico
Olivieri, Fabiola
author_facet Spazzafumo, Liana
Mensà, Emanuela
Matacchione, Giulia
Galeazzi, Tiziana
Zampini, Lucia
Recchioni, Rina
Marcheselli, Fiorella
Prattichizzo, Francesco
Testa, Roberto
Antonicelli, Roberto
Garagnani, Paolo
Boemi, Massimo
Bonafè, Massimiliano
Bonfigli, Anna Rita
Procopio, Antonio Domenico
Olivieri, Fabiola
author_sort Spazzafumo, Liana
collection PubMed
description β-Galactosidase (β-Gal) activity has been the most extensively utilized biomarker for the detection of cellular senescence. It can be measured also in plasma, and few recent evidence showed an altered plasmatic β-Gal activity in patients affected by some age-related diseases (ARDs). Since T2DM is one of the most common ARDs, we aimed to investigate if plasmatic β-Gal activity is modulated in T2DM patients and if “age” could affect such modulation. To gain mechanistic insights we paralleled this investigation with the evaluation of β-Gal activity in young and senescent endothelial cells (HUVECs) cultured in normo- and hyper-glycaemic environment. A significant age-related increase of plasmatic β-Gal activity was observed in healthy subjects (n. 230; 55-87 years), whereas the enzymatic activity was significantly reduced in T2DM patients (n. 230; 55-96 years) compared to healthy subjects. β-Gal activity detectable both in cells and in the culture medium was significantly increased in senescent cells compared to the younger ones, both under normo- and hyper-glycaemic condition. However, the hyper-glycaemic condition was not associated with an increased β-Gal activity in milieu compared to normo-glycaemic condition. Overall our data reinforce the notion that plasmatic β-Gal activity could be a systemic biomarker of aging, whereas T2DM patients are characterized by a different age-releated trend.
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spelling pubmed-57067992017-12-05 Age-related modulation of plasmatic beta-Galactosidase activity in healthy subjects and in patients affected by T2DM Spazzafumo, Liana Mensà, Emanuela Matacchione, Giulia Galeazzi, Tiziana Zampini, Lucia Recchioni, Rina Marcheselli, Fiorella Prattichizzo, Francesco Testa, Roberto Antonicelli, Roberto Garagnani, Paolo Boemi, Massimo Bonafè, Massimiliano Bonfigli, Anna Rita Procopio, Antonio Domenico Olivieri, Fabiola Oncotarget Research Paper: Gerotarget (Focus on Aging) β-Galactosidase (β-Gal) activity has been the most extensively utilized biomarker for the detection of cellular senescence. It can be measured also in plasma, and few recent evidence showed an altered plasmatic β-Gal activity in patients affected by some age-related diseases (ARDs). Since T2DM is one of the most common ARDs, we aimed to investigate if plasmatic β-Gal activity is modulated in T2DM patients and if “age” could affect such modulation. To gain mechanistic insights we paralleled this investigation with the evaluation of β-Gal activity in young and senescent endothelial cells (HUVECs) cultured in normo- and hyper-glycaemic environment. A significant age-related increase of plasmatic β-Gal activity was observed in healthy subjects (n. 230; 55-87 years), whereas the enzymatic activity was significantly reduced in T2DM patients (n. 230; 55-96 years) compared to healthy subjects. β-Gal activity detectable both in cells and in the culture medium was significantly increased in senescent cells compared to the younger ones, both under normo- and hyper-glycaemic condition. However, the hyper-glycaemic condition was not associated with an increased β-Gal activity in milieu compared to normo-glycaemic condition. Overall our data reinforce the notion that plasmatic β-Gal activity could be a systemic biomarker of aging, whereas T2DM patients are characterized by a different age-releated trend. Impact Journals LLC 2017-10-16 /pmc/articles/PMC5706799/ /pubmed/29212153 http://dx.doi.org/10.18632/oncotarget.21848 Text en Copyright: © 2017 Spazzafumo et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper: Gerotarget (Focus on Aging)
Spazzafumo, Liana
Mensà, Emanuela
Matacchione, Giulia
Galeazzi, Tiziana
Zampini, Lucia
Recchioni, Rina
Marcheselli, Fiorella
Prattichizzo, Francesco
Testa, Roberto
Antonicelli, Roberto
Garagnani, Paolo
Boemi, Massimo
Bonafè, Massimiliano
Bonfigli, Anna Rita
Procopio, Antonio Domenico
Olivieri, Fabiola
Age-related modulation of plasmatic beta-Galactosidase activity in healthy subjects and in patients affected by T2DM
title Age-related modulation of plasmatic beta-Galactosidase activity in healthy subjects and in patients affected by T2DM
title_full Age-related modulation of plasmatic beta-Galactosidase activity in healthy subjects and in patients affected by T2DM
title_fullStr Age-related modulation of plasmatic beta-Galactosidase activity in healthy subjects and in patients affected by T2DM
title_full_unstemmed Age-related modulation of plasmatic beta-Galactosidase activity in healthy subjects and in patients affected by T2DM
title_short Age-related modulation of plasmatic beta-Galactosidase activity in healthy subjects and in patients affected by T2DM
title_sort age-related modulation of plasmatic beta-galactosidase activity in healthy subjects and in patients affected by t2dm
topic Research Paper: Gerotarget (Focus on Aging)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706799/
https://www.ncbi.nlm.nih.gov/pubmed/29212153
http://dx.doi.org/10.18632/oncotarget.21848
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