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Low neighbor of Brca1 gene expression predicts poor clinical outcome and resistance of sunitinib in clear cell renal cell carcinoma
OBJECTIVE: To study the expression of Neighbor of Brca1 gene (NBR1) in clear cell renal cell carcinoma (ccRCC), renal cancer cells and the chemoresistance cells and to elucidate its clinical prognostic and chemoresistance value. MATERIALS AND METHODS: We screened the NBR1 mRNA in ccRCC from The Canc...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706915/ https://www.ncbi.nlm.nih.gov/pubmed/29212269 http://dx.doi.org/10.18632/oncotarget.21999 |
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author | Xiao, Wen Xiong, Zhiyong Yuan, Changfei Bao, Lin Liu, Di Yang, Xiong Li, Wencheng Tong, Junwei Qu, Yan Liu, Lei Xiao, Haibing Yang, Hongmei Zhang, Xiaoping Chen, Ke |
author_facet | Xiao, Wen Xiong, Zhiyong Yuan, Changfei Bao, Lin Liu, Di Yang, Xiong Li, Wencheng Tong, Junwei Qu, Yan Liu, Lei Xiao, Haibing Yang, Hongmei Zhang, Xiaoping Chen, Ke |
author_sort | Xiao, Wen |
collection | PubMed |
description | OBJECTIVE: To study the expression of Neighbor of Brca1 gene (NBR1) in clear cell renal cell carcinoma (ccRCC), renal cancer cells and the chemoresistance cells and to elucidate its clinical prognostic and chemoresistance value. MATERIALS AND METHODS: We screened the NBR1 mRNA in ccRCC from The Cancer Genome Atlas (TCGA) database and examined expression levels of NBR1 mRNA in 48 cases of ccRCC tissues, renal cancer cell lines and chemoresistance cells by qRT-PCR. Then, we extended two additional data sets in oncomine datebase (https://www.oncomine.org) to further confirm the results of the TCGA database. Immunohistochemistry (IHC) assay data performed in ccRCC tissues and normal tissues were downloaded from The Human Protein Atlas. RESULTS: The mRNA levels of NBR1 were downregulated in TCGA-KIRC database (n = 533) and ccRCC patient samples (n=48) as well as in RCC cell lines and their chemoresistance cells. Similarly, the protein levels of NBR1 were lower in ccRCC patient samples. NBR1 level was associated with the clinical pathological stage and could discriminate metastasis, recurrence and prognosis in ccRCC patients. Low level of NBR1 mRNA showed a significance poor prognostic of overall survival (OS), disease–free survival (DFS) with univariate and multivariate analyses in ccRCC patients and sunitinib resistance. CONCLUSIONS: Taken together, our results suggest that low level of NBR1 can predict poor clinical outcome and resistance of sunitinib in patients with ccRCC. |
format | Online Article Text |
id | pubmed-5706915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57069152017-12-05 Low neighbor of Brca1 gene expression predicts poor clinical outcome and resistance of sunitinib in clear cell renal cell carcinoma Xiao, Wen Xiong, Zhiyong Yuan, Changfei Bao, Lin Liu, Di Yang, Xiong Li, Wencheng Tong, Junwei Qu, Yan Liu, Lei Xiao, Haibing Yang, Hongmei Zhang, Xiaoping Chen, Ke Oncotarget Research Paper OBJECTIVE: To study the expression of Neighbor of Brca1 gene (NBR1) in clear cell renal cell carcinoma (ccRCC), renal cancer cells and the chemoresistance cells and to elucidate its clinical prognostic and chemoresistance value. MATERIALS AND METHODS: We screened the NBR1 mRNA in ccRCC from The Cancer Genome Atlas (TCGA) database and examined expression levels of NBR1 mRNA in 48 cases of ccRCC tissues, renal cancer cell lines and chemoresistance cells by qRT-PCR. Then, we extended two additional data sets in oncomine datebase (https://www.oncomine.org) to further confirm the results of the TCGA database. Immunohistochemistry (IHC) assay data performed in ccRCC tissues and normal tissues were downloaded from The Human Protein Atlas. RESULTS: The mRNA levels of NBR1 were downregulated in TCGA-KIRC database (n = 533) and ccRCC patient samples (n=48) as well as in RCC cell lines and their chemoresistance cells. Similarly, the protein levels of NBR1 were lower in ccRCC patient samples. NBR1 level was associated with the clinical pathological stage and could discriminate metastasis, recurrence and prognosis in ccRCC patients. Low level of NBR1 mRNA showed a significance poor prognostic of overall survival (OS), disease–free survival (DFS) with univariate and multivariate analyses in ccRCC patients and sunitinib resistance. CONCLUSIONS: Taken together, our results suggest that low level of NBR1 can predict poor clinical outcome and resistance of sunitinib in patients with ccRCC. Impact Journals LLC 2017-10-23 /pmc/articles/PMC5706915/ /pubmed/29212269 http://dx.doi.org/10.18632/oncotarget.21999 Text en Copyright: © 2017 Xiao et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xiao, Wen Xiong, Zhiyong Yuan, Changfei Bao, Lin Liu, Di Yang, Xiong Li, Wencheng Tong, Junwei Qu, Yan Liu, Lei Xiao, Haibing Yang, Hongmei Zhang, Xiaoping Chen, Ke Low neighbor of Brca1 gene expression predicts poor clinical outcome and resistance of sunitinib in clear cell renal cell carcinoma |
title | Low neighbor of Brca1 gene expression predicts poor clinical outcome and resistance of sunitinib in clear cell renal cell carcinoma |
title_full | Low neighbor of Brca1 gene expression predicts poor clinical outcome and resistance of sunitinib in clear cell renal cell carcinoma |
title_fullStr | Low neighbor of Brca1 gene expression predicts poor clinical outcome and resistance of sunitinib in clear cell renal cell carcinoma |
title_full_unstemmed | Low neighbor of Brca1 gene expression predicts poor clinical outcome and resistance of sunitinib in clear cell renal cell carcinoma |
title_short | Low neighbor of Brca1 gene expression predicts poor clinical outcome and resistance of sunitinib in clear cell renal cell carcinoma |
title_sort | low neighbor of brca1 gene expression predicts poor clinical outcome and resistance of sunitinib in clear cell renal cell carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706915/ https://www.ncbi.nlm.nih.gov/pubmed/29212269 http://dx.doi.org/10.18632/oncotarget.21999 |
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