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Histopathological, immunohistochemical, and ultrastructural evidence of spontaneous Senecavirus A-induced lesions at the choroid plexus of newborn piglets

Epidemic Transient Neonatal Losses (ETNL) is a disease of piglets caused by Senecavirus A (SVA) in which the method of dissemination and associated lesions are not well-defined. This study investigated the possible SVA-induced lesions by examining spontaneous infections in newborn piglets. Histopath...

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Autores principales: Oliveira, Thalita E. S., Michelazzo, Mariana M. Z., Fernandes, Thiago, de Oliveira, Admilton G., Leme, Raquel A., Alfieri, Alice F., Alfieri, Amauri A., Headley, Selwyn A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707367/
https://www.ncbi.nlm.nih.gov/pubmed/29185462
http://dx.doi.org/10.1038/s41598-017-16407-0
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author Oliveira, Thalita E. S.
Michelazzo, Mariana M. Z.
Fernandes, Thiago
de Oliveira, Admilton G.
Leme, Raquel A.
Alfieri, Alice F.
Alfieri, Amauri A.
Headley, Selwyn A.
author_facet Oliveira, Thalita E. S.
Michelazzo, Mariana M. Z.
Fernandes, Thiago
de Oliveira, Admilton G.
Leme, Raquel A.
Alfieri, Alice F.
Alfieri, Amauri A.
Headley, Selwyn A.
author_sort Oliveira, Thalita E. S.
collection PubMed
description Epidemic Transient Neonatal Losses (ETNL) is a disease of piglets caused by Senecavirus A (SVA) in which the method of dissemination and associated lesions are not well-defined. This study investigated the possible SVA-induced lesions by examining spontaneous infections in newborn piglets. Histopathology revealed ballooning degeneration of transitional epithelium, nonsuppurative meningoencephalitis, plexus choroiditis, and atrophic enteritis. RT-PCR identified SVA in all tissues evaluated and sequencing confirmed these results. Positive immunoreactivity to SVA was observed in endothelial and epithelial tissues of all organs evaluated. Semithin analysis revealed vacuolization of apical enterocytes of the small intestine, balloon degeneration and necrosis of endothelial cells of the choroid plexus (CP) and nonsuppurative choroid plexitis. Ultrathin evaluation demonstrated hydropic degeneration of apical enterocytes, degeneration and necrosis of endothelium of CP fenestrated capillaries, degeneration of ependymocytes associated with intralesional viral particles. It is proposed that SVA initially infects apical enterocytes of newborn piglets and probably enters the circulatory system with entry to the brain via the CP, by first producing an initial inflammatory reaction, with subsequent encephalitic dissemination. Consequently, SVA probably uses an enteric-neurological method of dissemination.
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spelling pubmed-57073672017-12-06 Histopathological, immunohistochemical, and ultrastructural evidence of spontaneous Senecavirus A-induced lesions at the choroid plexus of newborn piglets Oliveira, Thalita E. S. Michelazzo, Mariana M. Z. Fernandes, Thiago de Oliveira, Admilton G. Leme, Raquel A. Alfieri, Alice F. Alfieri, Amauri A. Headley, Selwyn A. Sci Rep Article Epidemic Transient Neonatal Losses (ETNL) is a disease of piglets caused by Senecavirus A (SVA) in which the method of dissemination and associated lesions are not well-defined. This study investigated the possible SVA-induced lesions by examining spontaneous infections in newborn piglets. Histopathology revealed ballooning degeneration of transitional epithelium, nonsuppurative meningoencephalitis, plexus choroiditis, and atrophic enteritis. RT-PCR identified SVA in all tissues evaluated and sequencing confirmed these results. Positive immunoreactivity to SVA was observed in endothelial and epithelial tissues of all organs evaluated. Semithin analysis revealed vacuolization of apical enterocytes of the small intestine, balloon degeneration and necrosis of endothelial cells of the choroid plexus (CP) and nonsuppurative choroid plexitis. Ultrathin evaluation demonstrated hydropic degeneration of apical enterocytes, degeneration and necrosis of endothelium of CP fenestrated capillaries, degeneration of ependymocytes associated with intralesional viral particles. It is proposed that SVA initially infects apical enterocytes of newborn piglets and probably enters the circulatory system with entry to the brain via the CP, by first producing an initial inflammatory reaction, with subsequent encephalitic dissemination. Consequently, SVA probably uses an enteric-neurological method of dissemination. Nature Publishing Group UK 2017-11-29 /pmc/articles/PMC5707367/ /pubmed/29185462 http://dx.doi.org/10.1038/s41598-017-16407-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Oliveira, Thalita E. S.
Michelazzo, Mariana M. Z.
Fernandes, Thiago
de Oliveira, Admilton G.
Leme, Raquel A.
Alfieri, Alice F.
Alfieri, Amauri A.
Headley, Selwyn A.
Histopathological, immunohistochemical, and ultrastructural evidence of spontaneous Senecavirus A-induced lesions at the choroid plexus of newborn piglets
title Histopathological, immunohistochemical, and ultrastructural evidence of spontaneous Senecavirus A-induced lesions at the choroid plexus of newborn piglets
title_full Histopathological, immunohistochemical, and ultrastructural evidence of spontaneous Senecavirus A-induced lesions at the choroid plexus of newborn piglets
title_fullStr Histopathological, immunohistochemical, and ultrastructural evidence of spontaneous Senecavirus A-induced lesions at the choroid plexus of newborn piglets
title_full_unstemmed Histopathological, immunohistochemical, and ultrastructural evidence of spontaneous Senecavirus A-induced lesions at the choroid plexus of newborn piglets
title_short Histopathological, immunohistochemical, and ultrastructural evidence of spontaneous Senecavirus A-induced lesions at the choroid plexus of newborn piglets
title_sort histopathological, immunohistochemical, and ultrastructural evidence of spontaneous senecavirus a-induced lesions at the choroid plexus of newborn piglets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707367/
https://www.ncbi.nlm.nih.gov/pubmed/29185462
http://dx.doi.org/10.1038/s41598-017-16407-0
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