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Intracellular localization of nanoparticle dimers by chirality reversal

The intra- and extracellular positioning of plasmonic nanoparticles (NPs) can dramatically alter their curative/diagnostic abilities and medical outcomes. However, the inability of common spectroscopic identifiers to register the events of transmembrane transport denies their intracellular vs. extra...

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Autores principales: Sun, Maozhong, Xu, Liguang, Bahng, Joong Hwan, Kuang, Hua, Alben, Silas, Kotov, Nicholas A., Xu, Chuanlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707389/
https://www.ncbi.nlm.nih.gov/pubmed/29185441
http://dx.doi.org/10.1038/s41467-017-01337-2
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author Sun, Maozhong
Xu, Liguang
Bahng, Joong Hwan
Kuang, Hua
Alben, Silas
Kotov, Nicholas A.
Xu, Chuanlai
author_facet Sun, Maozhong
Xu, Liguang
Bahng, Joong Hwan
Kuang, Hua
Alben, Silas
Kotov, Nicholas A.
Xu, Chuanlai
author_sort Sun, Maozhong
collection PubMed
description The intra- and extracellular positioning of plasmonic nanoparticles (NPs) can dramatically alter their curative/diagnostic abilities and medical outcomes. However, the inability of common spectroscopic identifiers to register the events of transmembrane transport denies their intracellular vs. extracellular localization even for cell cultures. Here we show that the chiroptical activity of DNA-bridged NP dimers allows one to follow the process of internalization of the particles by the mammalian cells and to distinguish their extra- vs intra-cellular localizations by real-time spectroscopy in ensemble. Circular dichroism peaks in the visible range change from negative to positive during transmembrane transport. The chirality reversal is associated with a spontaneous twisting motion around the DNA bridge caused by the large change in electrostatic repulsion between NPs when the dimers move from interstitial fluid to cytosol. This finding opens the door for spectroscopic targeting of plasmonic nanodrugs and quantitative assessment of nanoscale interactions. The efficacy of dichroic targeting of chiral nanostructures for biomedical applications is exemplified here as photodynamic therapy of malignancies. The efficacy of cervical cancer cell elimination was drastically increased when circular polarization of incident photons matched to the preferential absorption of dimers localized inside the cancer cells, which is associated with the increased generation of reactive oxygen species and their preferential intracellular localization.
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spelling pubmed-57073892017-12-04 Intracellular localization of nanoparticle dimers by chirality reversal Sun, Maozhong Xu, Liguang Bahng, Joong Hwan Kuang, Hua Alben, Silas Kotov, Nicholas A. Xu, Chuanlai Nat Commun Article The intra- and extracellular positioning of plasmonic nanoparticles (NPs) can dramatically alter their curative/diagnostic abilities and medical outcomes. However, the inability of common spectroscopic identifiers to register the events of transmembrane transport denies their intracellular vs. extracellular localization even for cell cultures. Here we show that the chiroptical activity of DNA-bridged NP dimers allows one to follow the process of internalization of the particles by the mammalian cells and to distinguish their extra- vs intra-cellular localizations by real-time spectroscopy in ensemble. Circular dichroism peaks in the visible range change from negative to positive during transmembrane transport. The chirality reversal is associated with a spontaneous twisting motion around the DNA bridge caused by the large change in electrostatic repulsion between NPs when the dimers move from interstitial fluid to cytosol. This finding opens the door for spectroscopic targeting of plasmonic nanodrugs and quantitative assessment of nanoscale interactions. The efficacy of dichroic targeting of chiral nanostructures for biomedical applications is exemplified here as photodynamic therapy of malignancies. The efficacy of cervical cancer cell elimination was drastically increased when circular polarization of incident photons matched to the preferential absorption of dimers localized inside the cancer cells, which is associated with the increased generation of reactive oxygen species and their preferential intracellular localization. Nature Publishing Group UK 2017-11-29 /pmc/articles/PMC5707389/ /pubmed/29185441 http://dx.doi.org/10.1038/s41467-017-01337-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sun, Maozhong
Xu, Liguang
Bahng, Joong Hwan
Kuang, Hua
Alben, Silas
Kotov, Nicholas A.
Xu, Chuanlai
Intracellular localization of nanoparticle dimers by chirality reversal
title Intracellular localization of nanoparticle dimers by chirality reversal
title_full Intracellular localization of nanoparticle dimers by chirality reversal
title_fullStr Intracellular localization of nanoparticle dimers by chirality reversal
title_full_unstemmed Intracellular localization of nanoparticle dimers by chirality reversal
title_short Intracellular localization of nanoparticle dimers by chirality reversal
title_sort intracellular localization of nanoparticle dimers by chirality reversal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707389/
https://www.ncbi.nlm.nih.gov/pubmed/29185441
http://dx.doi.org/10.1038/s41467-017-01337-2
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