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Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation

The generation of gene-edited animals using the CRISPRs/Cas9 system is based on microinjection into zygotes which is inefficient, time consuming and demands high technical skills. We report the optimization of an electroporation method for intact rat zygotes using sgRNAs and Cas9 protein in combinat...

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Detalles Bibliográficos
Autores principales:	Remy, Séverine, Chenouard, Vanessa, Tesson, Laurent, Usal, Claire, Ménoret, Séverine, Brusselle, Lucas, Heslan, Jean-Marie, Nguyen, Tuan Huan, Bellien, Jeremy, Merot, Jean, De Cian, Anne, Giovannangeli, Carine, Concordet, Jean-Paul, Anegon, Ignacio
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707420/ https://www.ncbi.nlm.nih.gov/pubmed/29185448 http://dx.doi.org/10.1038/s41598-017-16328-y

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