Chemical speciation of MeHg(+) and Hg(2+) in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes

Selected new fluorogenic probes that interact in different ways with Hg(2+) and MeHg(+) have been prepared and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best selective speciation of Hg(2+) and MeHg(+) has been achieved by in vitro approaches...

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Autores principales: Díaz de Greñu, Borja, García-Calvo, José, Cuevas, José, García-Herbosa, Gabriel, García, Begoña, Busto, Natalia, Ibeas, Saturnino, Torroba, Tomás, Torroba, Blanca, Herrera, Antonio, Pons, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707467/
https://www.ncbi.nlm.nih.gov/pubmed/29218145
http://dx.doi.org/10.1039/c5sc00718f
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author Díaz de Greñu, Borja
García-Calvo, José
Cuevas, José
García-Herbosa, Gabriel
García, Begoña
Busto, Natalia
Ibeas, Saturnino
Torroba, Tomás
Torroba, Blanca
Herrera, Antonio
Pons, Sebastian
author_facet Díaz de Greñu, Borja
García-Calvo, José
Cuevas, José
García-Herbosa, Gabriel
García, Begoña
Busto, Natalia
Ibeas, Saturnino
Torroba, Tomás
Torroba, Blanca
Herrera, Antonio
Pons, Sebastian
author_sort Díaz de Greñu, Borja
collection PubMed
description Selected new fluorogenic probes that interact in different ways with Hg(2+) and MeHg(+) have been prepared and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best selective speciation of Hg(2+) and MeHg(+) has been achieved by in vitro approaches based on fluorogenic probes supported in cultured cells, due to the particular sensitivity of the HEK293 cells to permeation by Hg(2+), MeHg(+) and the fluorogenic probes. In particular, MeHg(+) was selectively detected in cell nuclei by probe JG45.
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spelling pubmed-57074672017-12-07 Chemical speciation of MeHg(+) and Hg(2+) in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes Díaz de Greñu, Borja García-Calvo, José Cuevas, José García-Herbosa, Gabriel García, Begoña Busto, Natalia Ibeas, Saturnino Torroba, Tomás Torroba, Blanca Herrera, Antonio Pons, Sebastian Chem Sci Chemistry Selected new fluorogenic probes that interact in different ways with Hg(2+) and MeHg(+) have been prepared and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best selective speciation of Hg(2+) and MeHg(+) has been achieved by in vitro approaches based on fluorogenic probes supported in cultured cells, due to the particular sensitivity of the HEK293 cells to permeation by Hg(2+), MeHg(+) and the fluorogenic probes. In particular, MeHg(+) was selectively detected in cell nuclei by probe JG45. Royal Society of Chemistry 2015-07-01 2015-04-30 /pmc/articles/PMC5707467/ /pubmed/29218145 http://dx.doi.org/10.1039/c5sc00718f Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Díaz de Greñu, Borja
García-Calvo, José
Cuevas, José
García-Herbosa, Gabriel
García, Begoña
Busto, Natalia
Ibeas, Saturnino
Torroba, Tomás
Torroba, Blanca
Herrera, Antonio
Pons, Sebastian
Chemical speciation of MeHg(+) and Hg(2+) in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes
title Chemical speciation of MeHg(+) and Hg(2+) in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes
title_full Chemical speciation of MeHg(+) and Hg(2+) in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes
title_fullStr Chemical speciation of MeHg(+) and Hg(2+) in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes
title_full_unstemmed Chemical speciation of MeHg(+) and Hg(2+) in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes
title_short Chemical speciation of MeHg(+) and Hg(2+) in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes
title_sort chemical speciation of mehg(+) and hg(2+) in aqueous solution and hek cells nuclei by means of dna interacting fluorogenic probes
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707467/
https://www.ncbi.nlm.nih.gov/pubmed/29218145
http://dx.doi.org/10.1039/c5sc00718f
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