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Rapid Construction of Complex Plant RNA Virus Infectious cDNA Clones for Agroinfection Using a Yeast-E. coli-Agrobacterium Shuttle Vector

The availability of infectious full-length clone is indispensable for reverse genetics studies of virus biology, pathology and construction of viral vectors. However, for RNA viruses with large genome sizes or those exhibiting inherent cloning difficulties, procedure to generate biologically active...

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Detalles Bibliográficos
Autores principales:	Sun, Kai, Zhao, Danyang, Liu, Yong, Huang, Changjun, Zhang, Wei, Li, Zhenghe
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707539/ https://www.ncbi.nlm.nih.gov/pubmed/29112135 http://dx.doi.org/10.3390/v9110332

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