Cargando…
Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats
BACKGROUND: Jia-Wei-Jiao-Tai-Wan (JWJTW), composed of Jiao-Tai-Wan (Cinnamomum cassia and Rhizoma coptidis) and other antidiabetic herbs, including Astragalus membranaceus, Herba Gynostemmatis, Radix Puerariae Lobatae, Folium Mori and Semen Trigonellae, is widely used to treat diabetes and has demon...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707914/ https://www.ncbi.nlm.nih.gov/pubmed/29187178 http://dx.doi.org/10.1186/s12906-017-2016-5 |
_version_ | 1783282542361706496 |
---|---|
author | Chen, Guang Yang, Xueping Yang, Xiaoyu Li, Lingli Luo, Jinlong Dong, Hui Xu, Lijun Yi, Ping Wang, Kaifu Zou, Xin Lu, Fuer |
author_facet | Chen, Guang Yang, Xueping Yang, Xiaoyu Li, Lingli Luo, Jinlong Dong, Hui Xu, Lijun Yi, Ping Wang, Kaifu Zou, Xin Lu, Fuer |
author_sort | Chen, Guang |
collection | PubMed |
description | BACKGROUND: Jia-Wei-Jiao-Tai-Wan (JWJTW), composed of Jiao-Tai-Wan (Cinnamomum cassia and Rhizoma coptidis) and other antidiabetic herbs, including Astragalus membranaceus, Herba Gynostemmatis, Radix Puerariae Lobatae, Folium Mori and Semen Trigonellae, is widely used to treat diabetes and has demonstrated a curative effect in the clinic, but the potential mechanism is unknown. This study aimed to explore the effects of JWJTW on diabetic rats and to clarify the underlying mechanism. METHODS: JWJTW was prepared, and the main components contained in the formula were identified by high-performance liquid chromatography (HPLC) fingerprint analysis. Diabetic rats induced by streptozotocin (STZ) and a high-sucrose-high-fat diet were treated with two concentrations of JWJTW (1.025 and 2.05 g/kg/d) for 100 days. The oral glucose tolerance test (OGTT), insulin release test (IRT) and insulin tolerance test (ITT) were performed to measure the glycometabolism of the diabetic rats at the end of the treatment period. Blood was collected to determine the serum lipid levels of the diabetic rats. Nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) were detected in pancreas homogenates to analyze the oxidative stress in the pancreata of diabetic rats, and the expression levels of pancreatic and duodenal homeobox 1 (PDX-1) and insulin in the pancreas were tested by Western blot to measure pancreatic islet function. In addition, Western blots were used to measure the expression of proteins related to the insulin signaling pathway in skeletal muscle of the diabetic rats. RESULTS: The results showed that the administration of JWJTW could ameliorate impairments in glucose tolerance, insulin release function and insulin tolerance in diabetic rats. JWJTW could also dose-dependently reduce serum lipid levels in diabetic rats. JWJTW restrained oxidative stress by decreasing the expression of NO and MDA and increasing the expression of SOD and GSH-px. JWJTW improved the function of pancreatic β cells by increasing PDX-1 and insulin expression. In addition, JWJTW restored the impaired insulin signaling; upregulated phospho-insulin receptor (pInsR) expression, insulin receptor substrate (IRS) tyrosine phosphorylation, phosphatidylinositol 3-kinase (PI3K) (p85), and glucose transporter 4 (GLUT4) expression; and downregulated the serine phosphorylation of IRS. CONCLUSIONS: This study suggests that JWJTW can ameliorate type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats. |
format | Online Article Text |
id | pubmed-5707914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57079142017-12-06 Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats Chen, Guang Yang, Xueping Yang, Xiaoyu Li, Lingli Luo, Jinlong Dong, Hui Xu, Lijun Yi, Ping Wang, Kaifu Zou, Xin Lu, Fuer BMC Complement Altern Med Research Article BACKGROUND: Jia-Wei-Jiao-Tai-Wan (JWJTW), composed of Jiao-Tai-Wan (Cinnamomum cassia and Rhizoma coptidis) and other antidiabetic herbs, including Astragalus membranaceus, Herba Gynostemmatis, Radix Puerariae Lobatae, Folium Mori and Semen Trigonellae, is widely used to treat diabetes and has demonstrated a curative effect in the clinic, but the potential mechanism is unknown. This study aimed to explore the effects of JWJTW on diabetic rats and to clarify the underlying mechanism. METHODS: JWJTW was prepared, and the main components contained in the formula were identified by high-performance liquid chromatography (HPLC) fingerprint analysis. Diabetic rats induced by streptozotocin (STZ) and a high-sucrose-high-fat diet were treated with two concentrations of JWJTW (1.025 and 2.05 g/kg/d) for 100 days. The oral glucose tolerance test (OGTT), insulin release test (IRT) and insulin tolerance test (ITT) were performed to measure the glycometabolism of the diabetic rats at the end of the treatment period. Blood was collected to determine the serum lipid levels of the diabetic rats. Nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) were detected in pancreas homogenates to analyze the oxidative stress in the pancreata of diabetic rats, and the expression levels of pancreatic and duodenal homeobox 1 (PDX-1) and insulin in the pancreas were tested by Western blot to measure pancreatic islet function. In addition, Western blots were used to measure the expression of proteins related to the insulin signaling pathway in skeletal muscle of the diabetic rats. RESULTS: The results showed that the administration of JWJTW could ameliorate impairments in glucose tolerance, insulin release function and insulin tolerance in diabetic rats. JWJTW could also dose-dependently reduce serum lipid levels in diabetic rats. JWJTW restrained oxidative stress by decreasing the expression of NO and MDA and increasing the expression of SOD and GSH-px. JWJTW improved the function of pancreatic β cells by increasing PDX-1 and insulin expression. In addition, JWJTW restored the impaired insulin signaling; upregulated phospho-insulin receptor (pInsR) expression, insulin receptor substrate (IRS) tyrosine phosphorylation, phosphatidylinositol 3-kinase (PI3K) (p85), and glucose transporter 4 (GLUT4) expression; and downregulated the serine phosphorylation of IRS. CONCLUSIONS: This study suggests that JWJTW can ameliorate type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats. BioMed Central 2017-11-29 /pmc/articles/PMC5707914/ /pubmed/29187178 http://dx.doi.org/10.1186/s12906-017-2016-5 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Chen, Guang Yang, Xueping Yang, Xiaoyu Li, Lingli Luo, Jinlong Dong, Hui Xu, Lijun Yi, Ping Wang, Kaifu Zou, Xin Lu, Fuer Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats |
title | Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats |
title_full | Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats |
title_fullStr | Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats |
title_full_unstemmed | Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats |
title_short | Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats |
title_sort | jia-wei-jiao-tai-wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707914/ https://www.ncbi.nlm.nih.gov/pubmed/29187178 http://dx.doi.org/10.1186/s12906-017-2016-5 |
work_keys_str_mv | AT chenguang jiaweijiaotaiwanamelioratestype2diabetesbyimprovingbcellfunctionandreducinginsulinresistanceindiabeticrats AT yangxueping jiaweijiaotaiwanamelioratestype2diabetesbyimprovingbcellfunctionandreducinginsulinresistanceindiabeticrats AT yangxiaoyu jiaweijiaotaiwanamelioratestype2diabetesbyimprovingbcellfunctionandreducinginsulinresistanceindiabeticrats AT lilingli jiaweijiaotaiwanamelioratestype2diabetesbyimprovingbcellfunctionandreducinginsulinresistanceindiabeticrats AT luojinlong jiaweijiaotaiwanamelioratestype2diabetesbyimprovingbcellfunctionandreducinginsulinresistanceindiabeticrats AT donghui jiaweijiaotaiwanamelioratestype2diabetesbyimprovingbcellfunctionandreducinginsulinresistanceindiabeticrats AT xulijun jiaweijiaotaiwanamelioratestype2diabetesbyimprovingbcellfunctionandreducinginsulinresistanceindiabeticrats AT yiping jiaweijiaotaiwanamelioratestype2diabetesbyimprovingbcellfunctionandreducinginsulinresistanceindiabeticrats AT wangkaifu jiaweijiaotaiwanamelioratestype2diabetesbyimprovingbcellfunctionandreducinginsulinresistanceindiabeticrats AT zouxin jiaweijiaotaiwanamelioratestype2diabetesbyimprovingbcellfunctionandreducinginsulinresistanceindiabeticrats AT lufuer jiaweijiaotaiwanamelioratestype2diabetesbyimprovingbcellfunctionandreducinginsulinresistanceindiabeticrats |