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Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7

In July 2014, an outbreak of Shiga toxin–producing Escherichia coli (STEC) O55:H7 in England involved 31 patients, 13 (42%) of whom had hemolytic uremic syndrome. Isolates were sequenced, and the sequences were compared with publicly available sequences of E. coli O55:H7 and O157:H7. A core-genome p...

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Autores principales: Schutz, Kyle, Cowley, Lauren A., Shaaban, Sharif, Carroll, Anne, McNamara, Eleanor, Gally, David L., Godbole, Gauri, Jenkins, Claire, Dallman, Timothy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708253/
https://www.ncbi.nlm.nih.gov/pubmed/29148397
http://dx.doi.org/10.3201/eid2312.170628
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author Schutz, Kyle
Cowley, Lauren A.
Shaaban, Sharif
Carroll, Anne
McNamara, Eleanor
Gally, David L.
Godbole, Gauri
Jenkins, Claire
Dallman, Timothy J.
author_facet Schutz, Kyle
Cowley, Lauren A.
Shaaban, Sharif
Carroll, Anne
McNamara, Eleanor
Gally, David L.
Godbole, Gauri
Jenkins, Claire
Dallman, Timothy J.
author_sort Schutz, Kyle
collection PubMed
description In July 2014, an outbreak of Shiga toxin–producing Escherichia coli (STEC) O55:H7 in England involved 31 patients, 13 (42%) of whom had hemolytic uremic syndrome. Isolates were sequenced, and the sequences were compared with publicly available sequences of E. coli O55:H7 and O157:H7. A core-genome phylogeny of the evolutionary history of the STEC O55:H7 outbreak strain revealed that the most parsimonious model was a progenitor enteropathogenic O55:H7 sorbitol-fermenting strain, lysogenized by a Shiga toxin (Stx) 2a–encoding phage, followed by loss of the ability to ferment sorbitol because of a non-sense mutation in srlA. The parallel, convergent evolutionary histories of STEC O157:H7 and STEC O55:H7 may indicate a common driver in the evolutionary process. Because emergence of STEC O157:H7 as a clinically significant pathogen was associated with acquisition of the Stx2a-encoding phage, the emergence of STEC O55:H7 harboring the stx2a gene is of public health concern.
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spelling pubmed-57082532017-12-09 Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7 Schutz, Kyle Cowley, Lauren A. Shaaban, Sharif Carroll, Anne McNamara, Eleanor Gally, David L. Godbole, Gauri Jenkins, Claire Dallman, Timothy J. Emerg Infect Dis Research In July 2014, an outbreak of Shiga toxin–producing Escherichia coli (STEC) O55:H7 in England involved 31 patients, 13 (42%) of whom had hemolytic uremic syndrome. Isolates were sequenced, and the sequences were compared with publicly available sequences of E. coli O55:H7 and O157:H7. A core-genome phylogeny of the evolutionary history of the STEC O55:H7 outbreak strain revealed that the most parsimonious model was a progenitor enteropathogenic O55:H7 sorbitol-fermenting strain, lysogenized by a Shiga toxin (Stx) 2a–encoding phage, followed by loss of the ability to ferment sorbitol because of a non-sense mutation in srlA. The parallel, convergent evolutionary histories of STEC O157:H7 and STEC O55:H7 may indicate a common driver in the evolutionary process. Because emergence of STEC O157:H7 as a clinically significant pathogen was associated with acquisition of the Stx2a-encoding phage, the emergence of STEC O55:H7 harboring the stx2a gene is of public health concern. Centers for Disease Control and Prevention 2017-12 /pmc/articles/PMC5708253/ /pubmed/29148397 http://dx.doi.org/10.3201/eid2312.170628 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Schutz, Kyle
Cowley, Lauren A.
Shaaban, Sharif
Carroll, Anne
McNamara, Eleanor
Gally, David L.
Godbole, Gauri
Jenkins, Claire
Dallman, Timothy J.
Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7
title Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7
title_full Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7
title_fullStr Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7
title_full_unstemmed Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7
title_short Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7
title_sort evolutionary context of non–sorbitol-fermenting shiga toxin–producing escherichia coli o55:h7
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708253/
https://www.ncbi.nlm.nih.gov/pubmed/29148397
http://dx.doi.org/10.3201/eid2312.170628
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