Cargando…
Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7
In July 2014, an outbreak of Shiga toxin–producing Escherichia coli (STEC) O55:H7 in England involved 31 patients, 13 (42%) of whom had hemolytic uremic syndrome. Isolates were sequenced, and the sequences were compared with publicly available sequences of E. coli O55:H7 and O157:H7. A core-genome p...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Centers for Disease Control and Prevention
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708253/ https://www.ncbi.nlm.nih.gov/pubmed/29148397 http://dx.doi.org/10.3201/eid2312.170628 |
_version_ | 1783282612579598336 |
---|---|
author | Schutz, Kyle Cowley, Lauren A. Shaaban, Sharif Carroll, Anne McNamara, Eleanor Gally, David L. Godbole, Gauri Jenkins, Claire Dallman, Timothy J. |
author_facet | Schutz, Kyle Cowley, Lauren A. Shaaban, Sharif Carroll, Anne McNamara, Eleanor Gally, David L. Godbole, Gauri Jenkins, Claire Dallman, Timothy J. |
author_sort | Schutz, Kyle |
collection | PubMed |
description | In July 2014, an outbreak of Shiga toxin–producing Escherichia coli (STEC) O55:H7 in England involved 31 patients, 13 (42%) of whom had hemolytic uremic syndrome. Isolates were sequenced, and the sequences were compared with publicly available sequences of E. coli O55:H7 and O157:H7. A core-genome phylogeny of the evolutionary history of the STEC O55:H7 outbreak strain revealed that the most parsimonious model was a progenitor enteropathogenic O55:H7 sorbitol-fermenting strain, lysogenized by a Shiga toxin (Stx) 2a–encoding phage, followed by loss of the ability to ferment sorbitol because of a non-sense mutation in srlA. The parallel, convergent evolutionary histories of STEC O157:H7 and STEC O55:H7 may indicate a common driver in the evolutionary process. Because emergence of STEC O157:H7 as a clinically significant pathogen was associated with acquisition of the Stx2a-encoding phage, the emergence of STEC O55:H7 harboring the stx2a gene is of public health concern. |
format | Online Article Text |
id | pubmed-5708253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-57082532017-12-09 Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7 Schutz, Kyle Cowley, Lauren A. Shaaban, Sharif Carroll, Anne McNamara, Eleanor Gally, David L. Godbole, Gauri Jenkins, Claire Dallman, Timothy J. Emerg Infect Dis Research In July 2014, an outbreak of Shiga toxin–producing Escherichia coli (STEC) O55:H7 in England involved 31 patients, 13 (42%) of whom had hemolytic uremic syndrome. Isolates were sequenced, and the sequences were compared with publicly available sequences of E. coli O55:H7 and O157:H7. A core-genome phylogeny of the evolutionary history of the STEC O55:H7 outbreak strain revealed that the most parsimonious model was a progenitor enteropathogenic O55:H7 sorbitol-fermenting strain, lysogenized by a Shiga toxin (Stx) 2a–encoding phage, followed by loss of the ability to ferment sorbitol because of a non-sense mutation in srlA. The parallel, convergent evolutionary histories of STEC O157:H7 and STEC O55:H7 may indicate a common driver in the evolutionary process. Because emergence of STEC O157:H7 as a clinically significant pathogen was associated with acquisition of the Stx2a-encoding phage, the emergence of STEC O55:H7 harboring the stx2a gene is of public health concern. Centers for Disease Control and Prevention 2017-12 /pmc/articles/PMC5708253/ /pubmed/29148397 http://dx.doi.org/10.3201/eid2312.170628 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Schutz, Kyle Cowley, Lauren A. Shaaban, Sharif Carroll, Anne McNamara, Eleanor Gally, David L. Godbole, Gauri Jenkins, Claire Dallman, Timothy J. Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7 |
title | Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7 |
title_full | Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7 |
title_fullStr | Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7 |
title_full_unstemmed | Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7 |
title_short | Evolutionary Context of Non–Sorbitol-Fermenting Shiga Toxin–Producing Escherichia coli O55:H7 |
title_sort | evolutionary context of non–sorbitol-fermenting shiga toxin–producing escherichia coli o55:h7 |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708253/ https://www.ncbi.nlm.nih.gov/pubmed/29148397 http://dx.doi.org/10.3201/eid2312.170628 |
work_keys_str_mv | AT schutzkyle evolutionarycontextofnonsorbitolfermentingshigatoxinproducingescherichiacolio55h7 AT cowleylaurena evolutionarycontextofnonsorbitolfermentingshigatoxinproducingescherichiacolio55h7 AT shaabansharif evolutionarycontextofnonsorbitolfermentingshigatoxinproducingescherichiacolio55h7 AT carrollanne evolutionarycontextofnonsorbitolfermentingshigatoxinproducingescherichiacolio55h7 AT mcnamaraeleanor evolutionarycontextofnonsorbitolfermentingshigatoxinproducingescherichiacolio55h7 AT gallydavidl evolutionarycontextofnonsorbitolfermentingshigatoxinproducingescherichiacolio55h7 AT godbolegauri evolutionarycontextofnonsorbitolfermentingshigatoxinproducingescherichiacolio55h7 AT jenkinsclaire evolutionarycontextofnonsorbitolfermentingshigatoxinproducingescherichiacolio55h7 AT dallmantimothyj evolutionarycontextofnonsorbitolfermentingshigatoxinproducingescherichiacolio55h7 |