Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal

We describe an approach to generating and verifying well-defined redox states in metalloprotein single crystals by combining electrochemical control with synchrotron infrared microspectroscopic imaging. For NiFe hydrogenase 1 from Escherichia coli we demonstrate fully reversible and uniform electroc...

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Detalles Bibliográficos
Autores principales: Ash, P. A., Carr, S. B., Reeve, H. A., Skorupskaitė, A., Rowbotham, J. S., Shutt, R., Frogley, M. D., Evans, R. M., Cinque, G., Armstrong, F. A., Vincent, K. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708527/
https://www.ncbi.nlm.nih.gov/pubmed/28504793
http://dx.doi.org/10.1039/c7cc02591b
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author Ash, P. A.
Carr, S. B.
Reeve, H. A.
Skorupskaitė, A.
Rowbotham, J. S.
Shutt, R.
Frogley, M. D.
Evans, R. M.
Cinque, G.
Armstrong, F. A.
Vincent, K. A.
author_facet Ash, P. A.
Carr, S. B.
Reeve, H. A.
Skorupskaitė, A.
Rowbotham, J. S.
Shutt, R.
Frogley, M. D.
Evans, R. M.
Cinque, G.
Armstrong, F. A.
Vincent, K. A.
author_sort Ash, P. A.
collection PubMed
description We describe an approach to generating and verifying well-defined redox states in metalloprotein single crystals by combining electrochemical control with synchrotron infrared microspectroscopic imaging. For NiFe hydrogenase 1 from Escherichia coli we demonstrate fully reversible and uniform electrochemical reduction from the oxidised inactive to the fully reduced state, and temporally resolve steps during this reduction.
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spelling pubmed-57085272018-01-05 Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal Ash, P. A. Carr, S. B. Reeve, H. A. Skorupskaitė, A. Rowbotham, J. S. Shutt, R. Frogley, M. D. Evans, R. M. Cinque, G. Armstrong, F. A. Vincent, K. A. Chem Commun (Camb) Chemistry We describe an approach to generating and verifying well-defined redox states in metalloprotein single crystals by combining electrochemical control with synchrotron infrared microspectroscopic imaging. For NiFe hydrogenase 1 from Escherichia coli we demonstrate fully reversible and uniform electrochemical reduction from the oxidised inactive to the fully reduced state, and temporally resolve steps during this reduction. Royal Society of Chemistry 2017-05-28 2017-05-09 /pmc/articles/PMC5708527/ /pubmed/28504793 http://dx.doi.org/10.1039/c7cc02591b Text en This journal is © The Royal Society of Chemistry 2017 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Ash, P. A.
Carr, S. B.
Reeve, H. A.
Skorupskaitė, A.
Rowbotham, J. S.
Shutt, R.
Frogley, M. D.
Evans, R. M.
Cinque, G.
Armstrong, F. A.
Vincent, K. A.
Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal
title Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal
title_full Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal
title_fullStr Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal
title_full_unstemmed Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal
title_short Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal
title_sort generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a nife hydrogenase crystal
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708527/
https://www.ncbi.nlm.nih.gov/pubmed/28504793
http://dx.doi.org/10.1039/c7cc02591b
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