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Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal
We describe an approach to generating and verifying well-defined redox states in metalloprotein single crystals by combining electrochemical control with synchrotron infrared microspectroscopic imaging. For NiFe hydrogenase 1 from Escherichia coli we demonstrate fully reversible and uniform electroc...
Autores principales: | Ash, P. A., Carr, S. B., Reeve, H. A., Skorupskaitė, A., Rowbotham, J. S., Shutt, R., Frogley, M. D., Evans, R. M., Cinque, G., Armstrong, F. A., Vincent, K. A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708527/ https://www.ncbi.nlm.nih.gov/pubmed/28504793 http://dx.doi.org/10.1039/c7cc02591b |
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