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Replication capacity and adaptability of a severe fever with thrombocytopenia syndrome virus at different temperatures

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging disease caused by the SFTS virus (SFTSV). Although fever and thrombocytopenia are the typical manifestations of SFTS, a specific SFTS case with no fever was observed in Zhejiang, China. In this report, we aimed to explore the probable...

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Autores principales: Feng, Yan, Xu, Changping, Li, Cixiu, Lin, Junfen, Wang, Zhongfa, Zhang, Yanjun, Jiang, Jianmin, Lu, Yiyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708652/
https://www.ncbi.nlm.nih.gov/pubmed/29190712
http://dx.doi.org/10.1371/journal.pone.0188462
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author Feng, Yan
Xu, Changping
Li, Cixiu
Lin, Junfen
Wang, Zhongfa
Zhang, Yanjun
Jiang, Jianmin
Lu, Yiyu
author_facet Feng, Yan
Xu, Changping
Li, Cixiu
Lin, Junfen
Wang, Zhongfa
Zhang, Yanjun
Jiang, Jianmin
Lu, Yiyu
author_sort Feng, Yan
collection PubMed
description Severe fever with thrombocytopenia syndrome (SFTS) is an emerging disease caused by the SFTS virus (SFTSV). Although fever and thrombocytopenia are the typical manifestations of SFTS, a specific SFTS case with no fever was observed in Zhejiang, China. In this report, we aimed to explore the probable reason for the absence of fever by analyzing the genetic characteristics and temperature sensitivity (ts) of the SFTSV strain ZJ2013-06, which was isolated from the specific case. Phylogenetically, different clusters of SFTSV strains circulated in Zhejiang. ZJ2013-06 was farthest from ZJ2014-02, an isolate belonging to a Chinese dominant cluster, and nearest to the coastal strain NB24/CHN/2013. Ts tests, performed on Vero cells at 37°C and 39°C, indicated that ZJ2013-06 had restricted replication at 39°C. Its viral loads were substantially reduced at 39°C compared with that at 37°C (approximately 100-fold reduction) and were significantly lower than that of ZJ2014-02 at 39°C (P < 0.01). By adaptive culture at 39°C, the induced strain ZJ2013-06-P7 was obtained. Owing to a reverse mutation (S1616), ZJ2013-06-P7 lost the ts of the original strain, displaying similar replication processes with NB24/CHN/2013. The results indicated that the amino acid residue 1616 was related to the ts characteristics of ZJ2013-06. Our study revealed that ZJ2013-06 was temperature-sensitive and may be related to the absence of fever in our case.
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spelling pubmed-57086522017-12-15 Replication capacity and adaptability of a severe fever with thrombocytopenia syndrome virus at different temperatures Feng, Yan Xu, Changping Li, Cixiu Lin, Junfen Wang, Zhongfa Zhang, Yanjun Jiang, Jianmin Lu, Yiyu PLoS One Research Article Severe fever with thrombocytopenia syndrome (SFTS) is an emerging disease caused by the SFTS virus (SFTSV). Although fever and thrombocytopenia are the typical manifestations of SFTS, a specific SFTS case with no fever was observed in Zhejiang, China. In this report, we aimed to explore the probable reason for the absence of fever by analyzing the genetic characteristics and temperature sensitivity (ts) of the SFTSV strain ZJ2013-06, which was isolated from the specific case. Phylogenetically, different clusters of SFTSV strains circulated in Zhejiang. ZJ2013-06 was farthest from ZJ2014-02, an isolate belonging to a Chinese dominant cluster, and nearest to the coastal strain NB24/CHN/2013. Ts tests, performed on Vero cells at 37°C and 39°C, indicated that ZJ2013-06 had restricted replication at 39°C. Its viral loads were substantially reduced at 39°C compared with that at 37°C (approximately 100-fold reduction) and were significantly lower than that of ZJ2014-02 at 39°C (P < 0.01). By adaptive culture at 39°C, the induced strain ZJ2013-06-P7 was obtained. Owing to a reverse mutation (S1616), ZJ2013-06-P7 lost the ts of the original strain, displaying similar replication processes with NB24/CHN/2013. The results indicated that the amino acid residue 1616 was related to the ts characteristics of ZJ2013-06. Our study revealed that ZJ2013-06 was temperature-sensitive and may be related to the absence of fever in our case. Public Library of Science 2017-11-30 /pmc/articles/PMC5708652/ /pubmed/29190712 http://dx.doi.org/10.1371/journal.pone.0188462 Text en © 2017 Feng et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Feng, Yan
Xu, Changping
Li, Cixiu
Lin, Junfen
Wang, Zhongfa
Zhang, Yanjun
Jiang, Jianmin
Lu, Yiyu
Replication capacity and adaptability of a severe fever with thrombocytopenia syndrome virus at different temperatures
title Replication capacity and adaptability of a severe fever with thrombocytopenia syndrome virus at different temperatures
title_full Replication capacity and adaptability of a severe fever with thrombocytopenia syndrome virus at different temperatures
title_fullStr Replication capacity and adaptability of a severe fever with thrombocytopenia syndrome virus at different temperatures
title_full_unstemmed Replication capacity and adaptability of a severe fever with thrombocytopenia syndrome virus at different temperatures
title_short Replication capacity and adaptability of a severe fever with thrombocytopenia syndrome virus at different temperatures
title_sort replication capacity and adaptability of a severe fever with thrombocytopenia syndrome virus at different temperatures
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708652/
https://www.ncbi.nlm.nih.gov/pubmed/29190712
http://dx.doi.org/10.1371/journal.pone.0188462
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