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A Bayesian view of murine seminal cytokine networks

It has long been established that active agents in seminal fluid are key to initiating and coordinating mating-induced immunomodulation. This is in part governed by the actions of a network of cytokine interactions which, to date, remain largely undefined, and whose interspecific evolutionary conser...

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Autores principales: Johnson, Michelle L., Dasgupta, Tathagata, Gopichandran, Nadia, Field, Sarah L., Orsi, Nicolas M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708769/
https://www.ncbi.nlm.nih.gov/pubmed/29190674
http://dx.doi.org/10.1371/journal.pone.0188897
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author Johnson, Michelle L.
Dasgupta, Tathagata
Gopichandran, Nadia
Field, Sarah L.
Orsi, Nicolas M.
author_facet Johnson, Michelle L.
Dasgupta, Tathagata
Gopichandran, Nadia
Field, Sarah L.
Orsi, Nicolas M.
author_sort Johnson, Michelle L.
collection PubMed
description It has long been established that active agents in seminal fluid are key to initiating and coordinating mating-induced immunomodulation. This is in part governed by the actions of a network of cytokine interactions which, to date, remain largely undefined, and whose interspecific evolutionary conservation is unknown. This study applied Bayesian methods to illustrate the interrelationships between seminal profiles of interleukin (IL)-1alpha, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-17, eotaxin, granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage-colony stimulating factor (GM-CSF), interferon (IFN)-gamma, keratinocyte-derived chemokine (KC), monocyte chemoattractant protein (MCP-1), macrophage inflammatory protein (MIP-1) alpha, MIP-1beta, regulated on activation normal T cell expressed and secreted (RANTES), tumour necrosis factor (TNF)-alpha, leptin, inducible protein (IP)-10 and vascular endothelial growth factor (VEGF) in a rat model. IL-2, IL-9, IL-12 (p70), IL-13, IL-18, eotaxin, IFN-gamma, IP-10, KC, leptin, MCP-1, MIP-1alpha and TNF-alpha were significantly higher in serum, whilst IL-1beta, IL-5, IL-6, IL-10, IL-17, G-CSF and GM-CSF were significantly higher in seminal fluid. When compared to mouse profiles, only G-CSF was present at significantly higher levels in the seminal fluid in both species. Bayesian modelling highlighted key shared features across mouse and rat networks, namely TNF-alpha as the terminal node in both serum and seminal plasma, and MCP-1 as a central coordinator of seminal cytokine networks through the intermediary of KC and RANTES. These findings reveal a marked interspecific conservation of seminal cytokine networks.
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spelling pubmed-57087692017-12-15 A Bayesian view of murine seminal cytokine networks Johnson, Michelle L. Dasgupta, Tathagata Gopichandran, Nadia Field, Sarah L. Orsi, Nicolas M. PLoS One Research Article It has long been established that active agents in seminal fluid are key to initiating and coordinating mating-induced immunomodulation. This is in part governed by the actions of a network of cytokine interactions which, to date, remain largely undefined, and whose interspecific evolutionary conservation is unknown. This study applied Bayesian methods to illustrate the interrelationships between seminal profiles of interleukin (IL)-1alpha, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-17, eotaxin, granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage-colony stimulating factor (GM-CSF), interferon (IFN)-gamma, keratinocyte-derived chemokine (KC), monocyte chemoattractant protein (MCP-1), macrophage inflammatory protein (MIP-1) alpha, MIP-1beta, regulated on activation normal T cell expressed and secreted (RANTES), tumour necrosis factor (TNF)-alpha, leptin, inducible protein (IP)-10 and vascular endothelial growth factor (VEGF) in a rat model. IL-2, IL-9, IL-12 (p70), IL-13, IL-18, eotaxin, IFN-gamma, IP-10, KC, leptin, MCP-1, MIP-1alpha and TNF-alpha were significantly higher in serum, whilst IL-1beta, IL-5, IL-6, IL-10, IL-17, G-CSF and GM-CSF were significantly higher in seminal fluid. When compared to mouse profiles, only G-CSF was present at significantly higher levels in the seminal fluid in both species. Bayesian modelling highlighted key shared features across mouse and rat networks, namely TNF-alpha as the terminal node in both serum and seminal plasma, and MCP-1 as a central coordinator of seminal cytokine networks through the intermediary of KC and RANTES. These findings reveal a marked interspecific conservation of seminal cytokine networks. Public Library of Science 2017-11-30 /pmc/articles/PMC5708769/ /pubmed/29190674 http://dx.doi.org/10.1371/journal.pone.0188897 Text en © 2017 Johnson et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Johnson, Michelle L.
Dasgupta, Tathagata
Gopichandran, Nadia
Field, Sarah L.
Orsi, Nicolas M.
A Bayesian view of murine seminal cytokine networks
title A Bayesian view of murine seminal cytokine networks
title_full A Bayesian view of murine seminal cytokine networks
title_fullStr A Bayesian view of murine seminal cytokine networks
title_full_unstemmed A Bayesian view of murine seminal cytokine networks
title_short A Bayesian view of murine seminal cytokine networks
title_sort bayesian view of murine seminal cytokine networks
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708769/
https://www.ncbi.nlm.nih.gov/pubmed/29190674
http://dx.doi.org/10.1371/journal.pone.0188897
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