Flow cytometric discrimination of seven lineage markers by using two fluorochromes

Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4(+) and CD8(+) T cells, γδ T cells, B cells, NK cells and monocytes), using...

Descripción completa

Detalles Bibliográficos
Autores principales: Boin, Francesco, Giardino Torchia, Maria Letizia, Borrello, Ivan, Noonan, Kimberly A., Neil, Matthew, Soloski, Mark J., Cimbro, Raffaello
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708788/
https://www.ncbi.nlm.nih.gov/pubmed/29190813
http://dx.doi.org/10.1371/journal.pone.0188916
_version_ 1783282684229844992
author Boin, Francesco
Giardino Torchia, Maria Letizia
Borrello, Ivan
Noonan, Kimberly A.
Neil, Matthew
Soloski, Mark J.
Cimbro, Raffaello
author_facet Boin, Francesco
Giardino Torchia, Maria Letizia
Borrello, Ivan
Noonan, Kimberly A.
Neil, Matthew
Soloski, Mark J.
Cimbro, Raffaello
author_sort Boin, Francesco
collection PubMed
description Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4(+) and CD8(+) T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.
format Online
Article
Text
id pubmed-5708788
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-57087882017-12-15 Flow cytometric discrimination of seven lineage markers by using two fluorochromes Boin, Francesco Giardino Torchia, Maria Letizia Borrello, Ivan Noonan, Kimberly A. Neil, Matthew Soloski, Mark J. Cimbro, Raffaello PLoS One Research Article Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4(+) and CD8(+) T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping. Public Library of Science 2017-11-30 /pmc/articles/PMC5708788/ /pubmed/29190813 http://dx.doi.org/10.1371/journal.pone.0188916 Text en © 2017 Boin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Boin, Francesco
Giardino Torchia, Maria Letizia
Borrello, Ivan
Noonan, Kimberly A.
Neil, Matthew
Soloski, Mark J.
Cimbro, Raffaello
Flow cytometric discrimination of seven lineage markers by using two fluorochromes
title Flow cytometric discrimination of seven lineage markers by using two fluorochromes
title_full Flow cytometric discrimination of seven lineage markers by using two fluorochromes
title_fullStr Flow cytometric discrimination of seven lineage markers by using two fluorochromes
title_full_unstemmed Flow cytometric discrimination of seven lineage markers by using two fluorochromes
title_short Flow cytometric discrimination of seven lineage markers by using two fluorochromes
title_sort flow cytometric discrimination of seven lineage markers by using two fluorochromes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708788/
https://www.ncbi.nlm.nih.gov/pubmed/29190813
http://dx.doi.org/10.1371/journal.pone.0188916
work_keys_str_mv AT boinfrancesco flowcytometricdiscriminationofsevenlineagemarkersbyusingtwofluorochromes
AT giardinotorchiamarialetizia flowcytometricdiscriminationofsevenlineagemarkersbyusingtwofluorochromes
AT borrelloivan flowcytometricdiscriminationofsevenlineagemarkersbyusingtwofluorochromes
AT noonankimberlya flowcytometricdiscriminationofsevenlineagemarkersbyusingtwofluorochromes
AT neilmatthew flowcytometricdiscriminationofsevenlineagemarkersbyusingtwofluorochromes
AT soloskimarkj flowcytometricdiscriminationofsevenlineagemarkersbyusingtwofluorochromes
AT cimbroraffaello flowcytometricdiscriminationofsevenlineagemarkersbyusingtwofluorochromes

Ejemplares similares