Cargando…
Transcriptome analysis of WRKY gene family in Oryza officinalis Wall ex Watt and WRKY genes involved in responses to Xanthomonas oryzae pv. oryzae stress
Oryza officinalis Wall ex Watt, a very important and special wild rice species, shows abundant genetic diversity and disease resistance features, especially high resistance to bacterial blight. The molecular mechanisms of bacterial blight resistance in O. officinalis have not yet been elucidated. Th...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708796/ https://www.ncbi.nlm.nih.gov/pubmed/29190793 http://dx.doi.org/10.1371/journal.pone.0188742 |
_version_ | 1783282686118330368 |
---|---|
author | Jiang, Chunmiao Shen, Qingxi J. Wang, Bo He, Bin Xiao, Suqin Chen, Ling Yu, Tengqiong Ke, Xue Zhong, Qiaofang Fu, Jian Chen, Yue Wang, Lingxian Yin, Fuyou Zhang, Dunyu Ghidan, Walid Huang, Xingqi Cheng, Zaiquan |
author_facet | Jiang, Chunmiao Shen, Qingxi J. Wang, Bo He, Bin Xiao, Suqin Chen, Ling Yu, Tengqiong Ke, Xue Zhong, Qiaofang Fu, Jian Chen, Yue Wang, Lingxian Yin, Fuyou Zhang, Dunyu Ghidan, Walid Huang, Xingqi Cheng, Zaiquan |
author_sort | Jiang, Chunmiao |
collection | PubMed |
description | Oryza officinalis Wall ex Watt, a very important and special wild rice species, shows abundant genetic diversity and disease resistance features, especially high resistance to bacterial blight. The molecular mechanisms of bacterial blight resistance in O. officinalis have not yet been elucidated. The WRKY transcription factor family is one of the largest gene families involved in plant growth, development and stress response. However, little is known about the numbers, structure, molecular phylogenetics, and expression of the WRKY genes under Xanthomonas oryzae pv. oryzae (Xoo) stress in O. officinalis due to lacking of O. officinalis genome. Therefore, based on the RNA-sequencing data of O. officinalis, we performed a comprehensive study of WRKY genes in O. officinalis and identified 89 OoWRKY genes. Then 89 OoWRKY genes were classified into three groups based on the WRKY domains and zinc finger motifs. Phylogenetic analysis strongly supported that the evolution of OoWRKY genes were consistent with previous studies of WRKYs, and subgroup IIc OoWRKY genes were the original ancestors of some group II and group III OoWRKYs. Among the 89 OoWRKY genes, eight OoWRKYs displayed significantly different expression (>2-fold, p<0.01) in the O. officinalis transcriptome under Xoo strains PXO99 and C5 stress 48 h, suggesting these genes might play important role in PXO99 and C5 stress responses in O. officinalis. QRT-PCR analysis and confirmation of eight OoWRKYs expression patterns revealed that they responded strongly to PXO99 and C5 stress 24 h, 48 h, and 72 h, and the trends of these genes displaying marked changes were consistent with the 48 h RNA-sequencing data, demonstrated these genes played important roles in response to biotic stress and might even involved in the bacterial blight resistance. Tissue expression profiles of eight OoWRKY genes revealed that they were highly expressed in root, stem, leaf, and flower, especially in leaf (except OoWRKY71), suggesting these genes might be also important for plant growth and organ development. In this study, we analyzed the WRKY family of transcription factors in O.officinalis. Insight was gained into the classification, evolution, and function of the OoWRKY genes, revealing the putative roles of eight significantly different expression OoWRKYs in Xoo strains PXO99 and C5 stress responses in O.officinalis. This study provided a better understanding of the evolution and functions of O. officinalis WRKY genes, and suggested that manipulating eight significantly different expression OoWRKYs would enhance resistance to bacterial blight. |
format | Online Article Text |
id | pubmed-5708796 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57087962017-12-15 Transcriptome analysis of WRKY gene family in Oryza officinalis Wall ex Watt and WRKY genes involved in responses to Xanthomonas oryzae pv. oryzae stress Jiang, Chunmiao Shen, Qingxi J. Wang, Bo He, Bin Xiao, Suqin Chen, Ling Yu, Tengqiong Ke, Xue Zhong, Qiaofang Fu, Jian Chen, Yue Wang, Lingxian Yin, Fuyou Zhang, Dunyu Ghidan, Walid Huang, Xingqi Cheng, Zaiquan PLoS One Research Article Oryza officinalis Wall ex Watt, a very important and special wild rice species, shows abundant genetic diversity and disease resistance features, especially high resistance to bacterial blight. The molecular mechanisms of bacterial blight resistance in O. officinalis have not yet been elucidated. The WRKY transcription factor family is one of the largest gene families involved in plant growth, development and stress response. However, little is known about the numbers, structure, molecular phylogenetics, and expression of the WRKY genes under Xanthomonas oryzae pv. oryzae (Xoo) stress in O. officinalis due to lacking of O. officinalis genome. Therefore, based on the RNA-sequencing data of O. officinalis, we performed a comprehensive study of WRKY genes in O. officinalis and identified 89 OoWRKY genes. Then 89 OoWRKY genes were classified into three groups based on the WRKY domains and zinc finger motifs. Phylogenetic analysis strongly supported that the evolution of OoWRKY genes were consistent with previous studies of WRKYs, and subgroup IIc OoWRKY genes were the original ancestors of some group II and group III OoWRKYs. Among the 89 OoWRKY genes, eight OoWRKYs displayed significantly different expression (>2-fold, p<0.01) in the O. officinalis transcriptome under Xoo strains PXO99 and C5 stress 48 h, suggesting these genes might play important role in PXO99 and C5 stress responses in O. officinalis. QRT-PCR analysis and confirmation of eight OoWRKYs expression patterns revealed that they responded strongly to PXO99 and C5 stress 24 h, 48 h, and 72 h, and the trends of these genes displaying marked changes were consistent with the 48 h RNA-sequencing data, demonstrated these genes played important roles in response to biotic stress and might even involved in the bacterial blight resistance. Tissue expression profiles of eight OoWRKY genes revealed that they were highly expressed in root, stem, leaf, and flower, especially in leaf (except OoWRKY71), suggesting these genes might be also important for plant growth and organ development. In this study, we analyzed the WRKY family of transcription factors in O.officinalis. Insight was gained into the classification, evolution, and function of the OoWRKY genes, revealing the putative roles of eight significantly different expression OoWRKYs in Xoo strains PXO99 and C5 stress responses in O.officinalis. This study provided a better understanding of the evolution and functions of O. officinalis WRKY genes, and suggested that manipulating eight significantly different expression OoWRKYs would enhance resistance to bacterial blight. Public Library of Science 2017-11-30 /pmc/articles/PMC5708796/ /pubmed/29190793 http://dx.doi.org/10.1371/journal.pone.0188742 Text en © 2017 Jiang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Jiang, Chunmiao Shen, Qingxi J. Wang, Bo He, Bin Xiao, Suqin Chen, Ling Yu, Tengqiong Ke, Xue Zhong, Qiaofang Fu, Jian Chen, Yue Wang, Lingxian Yin, Fuyou Zhang, Dunyu Ghidan, Walid Huang, Xingqi Cheng, Zaiquan Transcriptome analysis of WRKY gene family in Oryza officinalis Wall ex Watt and WRKY genes involved in responses to Xanthomonas oryzae pv. oryzae stress |
title | Transcriptome analysis of WRKY gene family in Oryza officinalis Wall ex Watt and WRKY genes involved in responses to Xanthomonas oryzae pv. oryzae stress |
title_full | Transcriptome analysis of WRKY gene family in Oryza officinalis Wall ex Watt and WRKY genes involved in responses to Xanthomonas oryzae pv. oryzae stress |
title_fullStr | Transcriptome analysis of WRKY gene family in Oryza officinalis Wall ex Watt and WRKY genes involved in responses to Xanthomonas oryzae pv. oryzae stress |
title_full_unstemmed | Transcriptome analysis of WRKY gene family in Oryza officinalis Wall ex Watt and WRKY genes involved in responses to Xanthomonas oryzae pv. oryzae stress |
title_short | Transcriptome analysis of WRKY gene family in Oryza officinalis Wall ex Watt and WRKY genes involved in responses to Xanthomonas oryzae pv. oryzae stress |
title_sort | transcriptome analysis of wrky gene family in oryza officinalis wall ex watt and wrky genes involved in responses to xanthomonas oryzae pv. oryzae stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708796/ https://www.ncbi.nlm.nih.gov/pubmed/29190793 http://dx.doi.org/10.1371/journal.pone.0188742 |
work_keys_str_mv | AT jiangchunmiao transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT shenqingxij transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT wangbo transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT hebin transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT xiaosuqin transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT chenling transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT yutengqiong transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT kexue transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT zhongqiaofang transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT fujian transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT chenyue transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT wanglingxian transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT yinfuyou transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT zhangdunyu transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT ghidanwalid transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT huangxingqi transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress AT chengzaiquan transcriptomeanalysisofwrkygenefamilyinoryzaofficinaliswallexwattandwrkygenesinvolvedinresponsestoxanthomonasoryzaepvoryzaestress |