Improved Detection of Cytokines Produced by Invariant NKT Cells

Invariant Natural killer T (iNKT) cells rapidly produce copious amounts of multiple cytokines after in vivo activation, allowing for the direct detection of a number of cytokines directly ex vivo. However, for some cytokines this approach is suboptimal. Here, we report technical variations that allo...

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Autores principales: Sag, Duygu, Özkan, Müge, Kronenberg, Mitchell, Wingender, Gerhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709402/
https://www.ncbi.nlm.nih.gov/pubmed/29192280
http://dx.doi.org/10.1038/s41598-017-16832-1
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author Sag, Duygu
Özkan, Müge
Kronenberg, Mitchell
Wingender, Gerhard
author_facet Sag, Duygu
Özkan, Müge
Kronenberg, Mitchell
Wingender, Gerhard
author_sort Sag, Duygu
collection PubMed
description Invariant Natural killer T (iNKT) cells rapidly produce copious amounts of multiple cytokines after in vivo activation, allowing for the direct detection of a number of cytokines directly ex vivo. However, for some cytokines this approach is suboptimal. Here, we report technical variations that allow the improved detection of IL-4, IL-10, IL-13 and IL-17A ex vivo. Furthermore, we describe an alternative approach for stimulation of iNKT cells in vitro that allows a significantly improved detection of cytokines produced by iNKT cells. Together, these protocols allow the detection of iNKT cell cytokines ex vivo and in vitro with increased sensitivity.
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spelling pubmed-57094022017-12-06 Improved Detection of Cytokines Produced by Invariant NKT Cells Sag, Duygu Özkan, Müge Kronenberg, Mitchell Wingender, Gerhard Sci Rep Article Invariant Natural killer T (iNKT) cells rapidly produce copious amounts of multiple cytokines after in vivo activation, allowing for the direct detection of a number of cytokines directly ex vivo. However, for some cytokines this approach is suboptimal. Here, we report technical variations that allow the improved detection of IL-4, IL-10, IL-13 and IL-17A ex vivo. Furthermore, we describe an alternative approach for stimulation of iNKT cells in vitro that allows a significantly improved detection of cytokines produced by iNKT cells. Together, these protocols allow the detection of iNKT cell cytokines ex vivo and in vitro with increased sensitivity. Nature Publishing Group UK 2017-11-30 /pmc/articles/PMC5709402/ /pubmed/29192280 http://dx.doi.org/10.1038/s41598-017-16832-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sag, Duygu
Özkan, Müge
Kronenberg, Mitchell
Wingender, Gerhard
Improved Detection of Cytokines Produced by Invariant NKT Cells
title Improved Detection of Cytokines Produced by Invariant NKT Cells
title_full Improved Detection of Cytokines Produced by Invariant NKT Cells
title_fullStr Improved Detection of Cytokines Produced by Invariant NKT Cells
title_full_unstemmed Improved Detection of Cytokines Produced by Invariant NKT Cells
title_short Improved Detection of Cytokines Produced by Invariant NKT Cells
title_sort improved detection of cytokines produced by invariant nkt cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709402/
https://www.ncbi.nlm.nih.gov/pubmed/29192280
http://dx.doi.org/10.1038/s41598-017-16832-1
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