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Multiplex single-cell visualization of nucleic acids and protein during HIV infection

Technical limitations in simultaneous microscopic visualization of RNA, DNA, and proteins of HIV have curtailed progress in this field. To address this need we develop a microscopy approach, multiplex immunofluorescent cell-based detection of DNA, RNA and Protein (MICDDRP), which is based on branche...

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Detalles Bibliográficos
Autores principales: Puray-Chavez, Maritza, Tedbury, Philip R., Huber, Andrew D., Ukah, Obiaara B., Yapo, Vincent, Liu, Dandan, Ji, Juan, Wolf, Jennifer J., Engelman, Alan N., Sarafianos, Stefan G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709414/
https://www.ncbi.nlm.nih.gov/pubmed/29192235
http://dx.doi.org/10.1038/s41467-017-01693-z