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Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter
Cells acquire free metals through plasma membrane transporters. But, in natural settings, sequestering agents often render metals inaccessible to transporters, limiting metal bioavailability. Here we identify a pathway for metal acquisition, allowing cells to cope with this situation. Under limited...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709425/ https://www.ncbi.nlm.nih.gov/pubmed/29192218 http://dx.doi.org/10.1038/s41467-017-01930-5 |
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author | Klompmaker, Sandra H. Kohl, Kid Fasel, Nicolas Mayer, Andreas |
author_facet | Klompmaker, Sandra H. Kohl, Kid Fasel, Nicolas Mayer, Andreas |
author_sort | Klompmaker, Sandra H. |
collection | PubMed |
description | Cells acquire free metals through plasma membrane transporters. But, in natural settings, sequestering agents often render metals inaccessible to transporters, limiting metal bioavailability. Here we identify a pathway for metal acquisition, allowing cells to cope with this situation. Under limited bioavailability of Mg(2+), yeast cells upregulate fluid-phase endocytosis and transfer solutes from the environment into their vacuole, an acidocalcisome-like compartment loaded with highly concentrated polyphosphate. We propose that this anionic inorganic polymer, which is an avid chelator of Mg(2+), serves as an immobilized cation filter that accumulates Mg(2+) inside these organelles. It thus allows the vacuolar exporter Mnr2 to efficiently transfer Mg(2+) into the cytosol. Leishmania parasites also employ acidocalcisomal polyphosphate to multiply in their Mg(2+)-limited habitat, the phagolysosomes of inflammatory macrophages. This suggests that the pathway for metal uptake via endocytosis, acidocalcisomal polyphosphates and export into the cytosol, which we term EAPEC, is conserved. |
format | Online Article Text |
id | pubmed-5709425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57094252017-12-04 Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter Klompmaker, Sandra H. Kohl, Kid Fasel, Nicolas Mayer, Andreas Nat Commun Article Cells acquire free metals through plasma membrane transporters. But, in natural settings, sequestering agents often render metals inaccessible to transporters, limiting metal bioavailability. Here we identify a pathway for metal acquisition, allowing cells to cope with this situation. Under limited bioavailability of Mg(2+), yeast cells upregulate fluid-phase endocytosis and transfer solutes from the environment into their vacuole, an acidocalcisome-like compartment loaded with highly concentrated polyphosphate. We propose that this anionic inorganic polymer, which is an avid chelator of Mg(2+), serves as an immobilized cation filter that accumulates Mg(2+) inside these organelles. It thus allows the vacuolar exporter Mnr2 to efficiently transfer Mg(2+) into the cytosol. Leishmania parasites also employ acidocalcisomal polyphosphate to multiply in their Mg(2+)-limited habitat, the phagolysosomes of inflammatory macrophages. This suggests that the pathway for metal uptake via endocytosis, acidocalcisomal polyphosphates and export into the cytosol, which we term EAPEC, is conserved. Nature Publishing Group UK 2017-12-01 /pmc/articles/PMC5709425/ /pubmed/29192218 http://dx.doi.org/10.1038/s41467-017-01930-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Klompmaker, Sandra H. Kohl, Kid Fasel, Nicolas Mayer, Andreas Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter |
title | Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter |
title_full | Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter |
title_fullStr | Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter |
title_full_unstemmed | Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter |
title_short | Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter |
title_sort | magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709425/ https://www.ncbi.nlm.nih.gov/pubmed/29192218 http://dx.doi.org/10.1038/s41467-017-01930-5 |
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