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Chitosan-Graphene Oxide 3D scaffolds as Promising Tools for Bone Regeneration in Critical-Size Mouse Calvarial Defects

Limited self-regenerating capacity of human skeleton makes the reconstruction of critical size bone defect a significant challenge for clinical practice. Aimed for regenerating bone tissues, this study was designed to investigate osteogenic differentiation, along with bone repair capacity of 3D chit...

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Autores principales: Hermenean, Anca, Codreanu, Ada, Herman, Hildegard, Balta, Cornel, Rosu, Marcel, Mihali, Ciprian Valentin, Ivan, Alexandra, Dinescu, Sorina, Ionita, Mariana, Costache, Marieta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709492/
https://www.ncbi.nlm.nih.gov/pubmed/29192253
http://dx.doi.org/10.1038/s41598-017-16599-5
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author Hermenean, Anca
Codreanu, Ada
Herman, Hildegard
Balta, Cornel
Rosu, Marcel
Mihali, Ciprian Valentin
Ivan, Alexandra
Dinescu, Sorina
Ionita, Mariana
Costache, Marieta
author_facet Hermenean, Anca
Codreanu, Ada
Herman, Hildegard
Balta, Cornel
Rosu, Marcel
Mihali, Ciprian Valentin
Ivan, Alexandra
Dinescu, Sorina
Ionita, Mariana
Costache, Marieta
author_sort Hermenean, Anca
collection PubMed
description Limited self-regenerating capacity of human skeleton makes the reconstruction of critical size bone defect a significant challenge for clinical practice. Aimed for regenerating bone tissues, this study was designed to investigate osteogenic differentiation, along with bone repair capacity of 3D chitosan (CHT) scaffolds enriched with graphene oxide (GO) in critical-sized mouse calvarial defect. Histopathological/histomorphometry and scanning electron microscopy(SEM) analysis of the implants revealed larger amount of new bone in the CHT/GO-filled defects compared with CHT alone (p < 0.001). When combined with GO, CHT scaffolds synergistically promoted the increase of alkaline phosphatase activity both in vitro and in vivo experiments. This enhanced osteogenesis was corroborated with increased expression of bone morphogenetic protein (BMP) and Runx-2 up to week 4 post-implantation, which showed that GO facilitates the differentiation of osteoprogenitor cells. Meanwhile, osteogenesis was promoted by GO at the late stage as well, as indicated by the up-regulation of osteopontin and osteocalcin at week 8 and overexpressed at week 18, for both markers. Our data suggest that CHT/GO biomaterial could represent a promising tool for the reconstruction of large bone defects, without using exogenous living cells or growth factors.
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spelling pubmed-57094922017-12-06 Chitosan-Graphene Oxide 3D scaffolds as Promising Tools for Bone Regeneration in Critical-Size Mouse Calvarial Defects Hermenean, Anca Codreanu, Ada Herman, Hildegard Balta, Cornel Rosu, Marcel Mihali, Ciprian Valentin Ivan, Alexandra Dinescu, Sorina Ionita, Mariana Costache, Marieta Sci Rep Article Limited self-regenerating capacity of human skeleton makes the reconstruction of critical size bone defect a significant challenge for clinical practice. Aimed for regenerating bone tissues, this study was designed to investigate osteogenic differentiation, along with bone repair capacity of 3D chitosan (CHT) scaffolds enriched with graphene oxide (GO) in critical-sized mouse calvarial defect. Histopathological/histomorphometry and scanning electron microscopy(SEM) analysis of the implants revealed larger amount of new bone in the CHT/GO-filled defects compared with CHT alone (p < 0.001). When combined with GO, CHT scaffolds synergistically promoted the increase of alkaline phosphatase activity both in vitro and in vivo experiments. This enhanced osteogenesis was corroborated with increased expression of bone morphogenetic protein (BMP) and Runx-2 up to week 4 post-implantation, which showed that GO facilitates the differentiation of osteoprogenitor cells. Meanwhile, osteogenesis was promoted by GO at the late stage as well, as indicated by the up-regulation of osteopontin and osteocalcin at week 8 and overexpressed at week 18, for both markers. Our data suggest that CHT/GO biomaterial could represent a promising tool for the reconstruction of large bone defects, without using exogenous living cells or growth factors. Nature Publishing Group UK 2017-11-30 /pmc/articles/PMC5709492/ /pubmed/29192253 http://dx.doi.org/10.1038/s41598-017-16599-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hermenean, Anca
Codreanu, Ada
Herman, Hildegard
Balta, Cornel
Rosu, Marcel
Mihali, Ciprian Valentin
Ivan, Alexandra
Dinescu, Sorina
Ionita, Mariana
Costache, Marieta
Chitosan-Graphene Oxide 3D scaffolds as Promising Tools for Bone Regeneration in Critical-Size Mouse Calvarial Defects
title Chitosan-Graphene Oxide 3D scaffolds as Promising Tools for Bone Regeneration in Critical-Size Mouse Calvarial Defects
title_full Chitosan-Graphene Oxide 3D scaffolds as Promising Tools for Bone Regeneration in Critical-Size Mouse Calvarial Defects
title_fullStr Chitosan-Graphene Oxide 3D scaffolds as Promising Tools for Bone Regeneration in Critical-Size Mouse Calvarial Defects
title_full_unstemmed Chitosan-Graphene Oxide 3D scaffolds as Promising Tools for Bone Regeneration in Critical-Size Mouse Calvarial Defects
title_short Chitosan-Graphene Oxide 3D scaffolds as Promising Tools for Bone Regeneration in Critical-Size Mouse Calvarial Defects
title_sort chitosan-graphene oxide 3d scaffolds as promising tools for bone regeneration in critical-size mouse calvarial defects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709492/
https://www.ncbi.nlm.nih.gov/pubmed/29192253
http://dx.doi.org/10.1038/s41598-017-16599-5
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