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Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile
BACKGROUND: In eukaryotes, heterochromatin replicates late in S phase of the cell cycle and contains specific covalent modifications of histones. SuUR mutation found in Drosophila makes heterochromatin replicate earlier than in wild type and reduces the level of repressive histone modifications. SUU...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709859/ https://www.ncbi.nlm.nih.gov/pubmed/29191233 http://dx.doi.org/10.1186/s13072-017-0163-z |
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author | Posukh, Olga V. Maksimov, Daniil A. Laktionov, Petr P. Koryakov, Dmitry E. Belyakin, Stepan N. |
author_facet | Posukh, Olga V. Maksimov, Daniil A. Laktionov, Petr P. Koryakov, Dmitry E. Belyakin, Stepan N. |
author_sort | Posukh, Olga V. |
collection | PubMed |
description | BACKGROUND: In eukaryotes, heterochromatin replicates late in S phase of the cell cycle and contains specific covalent modifications of histones. SuUR mutation found in Drosophila makes heterochromatin replicate earlier than in wild type and reduces the level of repressive histone modifications. SUUR protein was shown to be associated with moving replication forks, apparently through the interaction with PCNA. The biological process underlying the effects of SUUR on replication and composition of heterochromatin remains unknown. RESULTS: Here we performed a functional dissection of SUUR protein effects on H3K27me3 level. Using hidden Markow model-based algorithm we revealed SuUR-sensitive chromosomal regions that demonstrated unusual characteristics: They do not contain Polycomb and require SUUR function to sustain H3K27me3 level. We tested the role of SUUR protein in the mechanisms that could affect H3K27me3 histone levels in these regions. We found that SUUR does not affect the initial H3K27me3 pattern formation in embryogenesis or Polycomb distribution in the chromosomes. We also ruled out the possible effect of SUUR on histone genes expression and its involvement in DSB repair. CONCLUSIONS: Obtained results support the idea that SUUR protein contributes to the heterochromatin maintenance during the chromosome replication. A model that explains major SUUR-associated phenotypes is proposed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13072-017-0163-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5709859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57098592017-12-06 Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile Posukh, Olga V. Maksimov, Daniil A. Laktionov, Petr P. Koryakov, Dmitry E. Belyakin, Stepan N. Epigenetics Chromatin Research BACKGROUND: In eukaryotes, heterochromatin replicates late in S phase of the cell cycle and contains specific covalent modifications of histones. SuUR mutation found in Drosophila makes heterochromatin replicate earlier than in wild type and reduces the level of repressive histone modifications. SUUR protein was shown to be associated with moving replication forks, apparently through the interaction with PCNA. The biological process underlying the effects of SUUR on replication and composition of heterochromatin remains unknown. RESULTS: Here we performed a functional dissection of SUUR protein effects on H3K27me3 level. Using hidden Markow model-based algorithm we revealed SuUR-sensitive chromosomal regions that demonstrated unusual characteristics: They do not contain Polycomb and require SUUR function to sustain H3K27me3 level. We tested the role of SUUR protein in the mechanisms that could affect H3K27me3 histone levels in these regions. We found that SUUR does not affect the initial H3K27me3 pattern formation in embryogenesis or Polycomb distribution in the chromosomes. We also ruled out the possible effect of SUUR on histone genes expression and its involvement in DSB repair. CONCLUSIONS: Obtained results support the idea that SUUR protein contributes to the heterochromatin maintenance during the chromosome replication. A model that explains major SUUR-associated phenotypes is proposed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13072-017-0163-z) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-01 /pmc/articles/PMC5709859/ /pubmed/29191233 http://dx.doi.org/10.1186/s13072-017-0163-z Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Posukh, Olga V. Maksimov, Daniil A. Laktionov, Petr P. Koryakov, Dmitry E. Belyakin, Stepan N. Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile |
title | Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile |
title_full | Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile |
title_fullStr | Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile |
title_full_unstemmed | Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile |
title_short | Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile |
title_sort | functional dissection of drosophila melanogaster suur protein influence on h3k27me3 profile |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709859/ https://www.ncbi.nlm.nih.gov/pubmed/29191233 http://dx.doi.org/10.1186/s13072-017-0163-z |
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