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(18)F-fluorodeoxyglucose uptake predicts MET expression in lung adenocarcinoma

OBJECTIVE: MET is a member of the receptor tyrosine kinases. Several MET-targeting inhibitors and antagonistic antibodies have shown promising data in clinical trials of lung adenocarcinoma. Finding noninvasive diagnostic tools to estimate the status of MET is helpful in clinical practice. (18)F-flu...

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Autores principales: An, Shuxian, Zhou, Xiang, Liu, Jianjun, Huang, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709992/
https://www.ncbi.nlm.nih.gov/pubmed/29225472
http://dx.doi.org/10.2147/OTT.S150334
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author An, Shuxian
Zhou, Xiang
Liu, Jianjun
Huang, Gang
author_facet An, Shuxian
Zhou, Xiang
Liu, Jianjun
Huang, Gang
author_sort An, Shuxian
collection PubMed
description OBJECTIVE: MET is a member of the receptor tyrosine kinases. Several MET-targeting inhibitors and antagonistic antibodies have shown promising data in clinical trials of lung adenocarcinoma. Finding noninvasive diagnostic tools to estimate the status of MET is helpful in clinical practice. (18)F-fluorodeoxyglucose positron emission tomography/computerized tomography ((18)F-FDG PET/CT) has been used routinely for the diagnosis and staging of tumors. However, the relationship between MET expression and (18)F-FDG uptake has not been investigated yet. This study aimed to determine the correlation of MET expression with (18)F-FDG uptake on PET-CT scan and whether or not (18)F-FDG PET/CT can be used to predict the MET status of lung adenocarcinoma patients. PATIENTS AND METHODS: Fifty-seven lung adenocarcinoma patients were analyzed in our study. Maximum standardized uptake value (SUV(max)) was calculated in all PET/CT images. The expression levels of MET and two important glycolysis-related markers, glucose transporter 1 (GLUT1) and pyruvate kinase M2, were analyzed by immunohistochemistry of tissues. Spearman rank correlation was used to analyze the association between MET expression and SUV(max). In vitro MET knockdown in lung adenocarcinoma cells was used to examine the role of MET in tumor metabolism. The effect of MET on GLUT1 expression was investigated using Western blot assay and quantitative polymerase chain reaction. RESULTS: SUV(max) was positively correlated with the expression levels of MET (r=0.458; P<0.001) and GLUT1 (r=0.551; P<0.001). SUV(max) was significantly higher in patients with positive MET expression than in those with negative MET expression (9.92±6.62 vs 4.60±3.00; P=0.002). MET knockdown in lung adenocarcinoma cells led to a significant decrease in GLUT1 expression and (18)F-FDG uptake. CONCLUSION: MET could increase (18)F-FDG uptake by upregulating GLUT1 expression. (18)F-FDG PET/CT could be used to predict the MET status of lung adenocarcinoma patients and to supply valuable information to guide targeted therapy.
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spelling pubmed-57099922017-12-08 (18)F-fluorodeoxyglucose uptake predicts MET expression in lung adenocarcinoma An, Shuxian Zhou, Xiang Liu, Jianjun Huang, Gang Onco Targets Ther Original Research OBJECTIVE: MET is a member of the receptor tyrosine kinases. Several MET-targeting inhibitors and antagonistic antibodies have shown promising data in clinical trials of lung adenocarcinoma. Finding noninvasive diagnostic tools to estimate the status of MET is helpful in clinical practice. (18)F-fluorodeoxyglucose positron emission tomography/computerized tomography ((18)F-FDG PET/CT) has been used routinely for the diagnosis and staging of tumors. However, the relationship between MET expression and (18)F-FDG uptake has not been investigated yet. This study aimed to determine the correlation of MET expression with (18)F-FDG uptake on PET-CT scan and whether or not (18)F-FDG PET/CT can be used to predict the MET status of lung adenocarcinoma patients. PATIENTS AND METHODS: Fifty-seven lung adenocarcinoma patients were analyzed in our study. Maximum standardized uptake value (SUV(max)) was calculated in all PET/CT images. The expression levels of MET and two important glycolysis-related markers, glucose transporter 1 (GLUT1) and pyruvate kinase M2, were analyzed by immunohistochemistry of tissues. Spearman rank correlation was used to analyze the association between MET expression and SUV(max). In vitro MET knockdown in lung adenocarcinoma cells was used to examine the role of MET in tumor metabolism. The effect of MET on GLUT1 expression was investigated using Western blot assay and quantitative polymerase chain reaction. RESULTS: SUV(max) was positively correlated with the expression levels of MET (r=0.458; P<0.001) and GLUT1 (r=0.551; P<0.001). SUV(max) was significantly higher in patients with positive MET expression than in those with negative MET expression (9.92±6.62 vs 4.60±3.00; P=0.002). MET knockdown in lung adenocarcinoma cells led to a significant decrease in GLUT1 expression and (18)F-FDG uptake. CONCLUSION: MET could increase (18)F-FDG uptake by upregulating GLUT1 expression. (18)F-FDG PET/CT could be used to predict the MET status of lung adenocarcinoma patients and to supply valuable information to guide targeted therapy. Dove Medical Press 2017-11-27 /pmc/articles/PMC5709992/ /pubmed/29225472 http://dx.doi.org/10.2147/OTT.S150334 Text en © 2017 An et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
An, Shuxian
Zhou, Xiang
Liu, Jianjun
Huang, Gang
(18)F-fluorodeoxyglucose uptake predicts MET expression in lung adenocarcinoma
title (18)F-fluorodeoxyglucose uptake predicts MET expression in lung adenocarcinoma
title_full (18)F-fluorodeoxyglucose uptake predicts MET expression in lung adenocarcinoma
title_fullStr (18)F-fluorodeoxyglucose uptake predicts MET expression in lung adenocarcinoma
title_full_unstemmed (18)F-fluorodeoxyglucose uptake predicts MET expression in lung adenocarcinoma
title_short (18)F-fluorodeoxyglucose uptake predicts MET expression in lung adenocarcinoma
title_sort (18)f-fluorodeoxyglucose uptake predicts met expression in lung adenocarcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709992/
https://www.ncbi.nlm.nih.gov/pubmed/29225472
http://dx.doi.org/10.2147/OTT.S150334
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