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A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation

Histone H3 Lys4 (H3K4) methylation is a chromatin feature enriched at gene cis-regulatory sequences such as promoters and enhancers. Here we identify an evolutionarily conserved factor, BRWD2/PHIP, which colocalizes with histone H3K4 methylation genome-wide in human cells, mouse embryonic stem cells...

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Autores principales: Morgan, Marc A.J., Rickels, Ryan A., Collings, Clayton K., He, Xiaolin, Cao, Kaixiang, Herz, Hans-Martin, Cozzolino, Kira A., Abshiru, Nebiyu A., Marshall, Stacy A., Rendleman, Emily J., Sze, Christie C., Piunti, Andrea, Kelleher, Neil L., Savas, Jeffrey N., Shilatifard, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5710144/
https://www.ncbi.nlm.nih.gov/pubmed/29089422
http://dx.doi.org/10.1101/gad.305201.117
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author Morgan, Marc A.J.
Rickels, Ryan A.
Collings, Clayton K.
He, Xiaolin
Cao, Kaixiang
Herz, Hans-Martin
Cozzolino, Kira A.
Abshiru, Nebiyu A.
Marshall, Stacy A.
Rendleman, Emily J.
Sze, Christie C.
Piunti, Andrea
Kelleher, Neil L.
Savas, Jeffrey N.
Shilatifard, Ali
author_facet Morgan, Marc A.J.
Rickels, Ryan A.
Collings, Clayton K.
He, Xiaolin
Cao, Kaixiang
Herz, Hans-Martin
Cozzolino, Kira A.
Abshiru, Nebiyu A.
Marshall, Stacy A.
Rendleman, Emily J.
Sze, Christie C.
Piunti, Andrea
Kelleher, Neil L.
Savas, Jeffrey N.
Shilatifard, Ali
author_sort Morgan, Marc A.J.
collection PubMed
description Histone H3 Lys4 (H3K4) methylation is a chromatin feature enriched at gene cis-regulatory sequences such as promoters and enhancers. Here we identify an evolutionarily conserved factor, BRWD2/PHIP, which colocalizes with histone H3K4 methylation genome-wide in human cells, mouse embryonic stem cells, and Drosophila. Biochemical analysis of BRWD2 demonstrated an association with the Cullin-4–RING ubiquitin E3 ligase-4 (CRL4) complex, nucleosomes, and chromatin remodelers. BRWD2/PHIP binds directly to H3K4 methylation through a previously unidentified chromatin-binding module related to Royal Family Tudor domains, which we named the CryptoTudor domain. Using CRISPR–Cas9 genetic knockouts, we demonstrate that COMPASS H3K4 methyltransferase family members differentially regulate BRWD2/PHIP chromatin occupancy. Finally, we demonstrate that depletion of the single Drosophila homolog dBRWD3 results in altered gene expression and aberrant patterns of histone H3 Lys27 acetylation at enhancers and promoters, suggesting a cross-talk between these chromatin modifications and transcription through the BRWD protein family.
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spelling pubmed-57101442018-04-01 A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation Morgan, Marc A.J. Rickels, Ryan A. Collings, Clayton K. He, Xiaolin Cao, Kaixiang Herz, Hans-Martin Cozzolino, Kira A. Abshiru, Nebiyu A. Marshall, Stacy A. Rendleman, Emily J. Sze, Christie C. Piunti, Andrea Kelleher, Neil L. Savas, Jeffrey N. Shilatifard, Ali Genes Dev Research Paper Histone H3 Lys4 (H3K4) methylation is a chromatin feature enriched at gene cis-regulatory sequences such as promoters and enhancers. Here we identify an evolutionarily conserved factor, BRWD2/PHIP, which colocalizes with histone H3K4 methylation genome-wide in human cells, mouse embryonic stem cells, and Drosophila. Biochemical analysis of BRWD2 demonstrated an association with the Cullin-4–RING ubiquitin E3 ligase-4 (CRL4) complex, nucleosomes, and chromatin remodelers. BRWD2/PHIP binds directly to H3K4 methylation through a previously unidentified chromatin-binding module related to Royal Family Tudor domains, which we named the CryptoTudor domain. Using CRISPR–Cas9 genetic knockouts, we demonstrate that COMPASS H3K4 methyltransferase family members differentially regulate BRWD2/PHIP chromatin occupancy. Finally, we demonstrate that depletion of the single Drosophila homolog dBRWD3 results in altered gene expression and aberrant patterns of histone H3 Lys27 acetylation at enhancers and promoters, suggesting a cross-talk between these chromatin modifications and transcription through the BRWD protein family. Cold Spring Harbor Laboratory Press 2017-10-01 /pmc/articles/PMC5710144/ /pubmed/29089422 http://dx.doi.org/10.1101/gad.305201.117 Text en © 2017 Morgan et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Paper
Morgan, Marc A.J.
Rickels, Ryan A.
Collings, Clayton K.
He, Xiaolin
Cao, Kaixiang
Herz, Hans-Martin
Cozzolino, Kira A.
Abshiru, Nebiyu A.
Marshall, Stacy A.
Rendleman, Emily J.
Sze, Christie C.
Piunti, Andrea
Kelleher, Neil L.
Savas, Jeffrey N.
Shilatifard, Ali
A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation
title A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation
title_full A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation
title_fullStr A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation
title_full_unstemmed A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation
title_short A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation
title_sort cryptic tudor domain links brwd2/phip to compass-mediated histone h3k4 methylation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5710144/
https://www.ncbi.nlm.nih.gov/pubmed/29089422
http://dx.doi.org/10.1101/gad.305201.117
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