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Phylogenetic Relationships Based on DNA Barcoding Among 16 Species of the Ant Genus Formica (Hymenoptera: Formicidae) from China

In this study, we sequenced fragments of cytochrome oxidase subunit 1 (CO1), internal transcribed spacer 1 (ITS1), and internal transcribed spacer 2 (ITS2) genes from 150 specimens belonging to 16 species of the ant genus Formica from China. Odontoponera transversa from Ponerinae and Polyergus samur...

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Autores principales: Chen, Yuan, Zhou, Shanyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5710517/
http://dx.doi.org/10.1093/jisesa/iex092
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author Chen, Yuan
Zhou, Shanyi
author_facet Chen, Yuan
Zhou, Shanyi
author_sort Chen, Yuan
collection PubMed
description In this study, we sequenced fragments of cytochrome oxidase subunit 1 (CO1), internal transcribed spacer 1 (ITS1), and internal transcribed spacer 2 (ITS2) genes from 150 specimens belonging to 16 species of the ant genus Formica from China. Odontoponera transversa from Ponerinae and Polyergus samurai from Formicinae were added as distant relative and close relative outgroups, respectively. Neighbor-joining, maximum parsimony, and Bayesian interference methods were used to analyze their phylogenetic relationships based on CO1 gene sequence as well as combined sequence data of CO1 + ITS1, CO1 + ITS2, and CO1 + ITS1 + ITS2. The results showed that nine Formica species (i.e., Formica sinensis, Formica manchu, Formica uralensis, Formica sanguinea, Formica gagatoides, Formica candida, Formica fusca, Formica glauca, and Formica sp.) formed monophyletic clades, which in agreement with the results based on morphological taxonomy. By comparing the results of DNA barcoding and morphological taxonomy, we propose that Formica aquilonia maybe a junior synonym of F. polyctena and that cryptic species could likely existed in Formica sinae. Further studies on morphology, biology, and geography are needed to confirm this notion.
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spelling pubmed-57105172017-12-07 Phylogenetic Relationships Based on DNA Barcoding Among 16 Species of the Ant Genus Formica (Hymenoptera: Formicidae) from China Chen, Yuan Zhou, Shanyi J Insect Sci Research In this study, we sequenced fragments of cytochrome oxidase subunit 1 (CO1), internal transcribed spacer 1 (ITS1), and internal transcribed spacer 2 (ITS2) genes from 150 specimens belonging to 16 species of the ant genus Formica from China. Odontoponera transversa from Ponerinae and Polyergus samurai from Formicinae were added as distant relative and close relative outgroups, respectively. Neighbor-joining, maximum parsimony, and Bayesian interference methods were used to analyze their phylogenetic relationships based on CO1 gene sequence as well as combined sequence data of CO1 + ITS1, CO1 + ITS2, and CO1 + ITS1 + ITS2. The results showed that nine Formica species (i.e., Formica sinensis, Formica manchu, Formica uralensis, Formica sanguinea, Formica gagatoides, Formica candida, Formica fusca, Formica glauca, and Formica sp.) formed monophyletic clades, which in agreement with the results based on morphological taxonomy. By comparing the results of DNA barcoding and morphological taxonomy, we propose that Formica aquilonia maybe a junior synonym of F. polyctena and that cryptic species could likely existed in Formica sinae. Further studies on morphology, biology, and geography are needed to confirm this notion. Oxford University Press 2017-11-29 /pmc/articles/PMC5710517/ http://dx.doi.org/10.1093/jisesa/iex092 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research
Chen, Yuan
Zhou, Shanyi
Phylogenetic Relationships Based on DNA Barcoding Among 16 Species of the Ant Genus Formica (Hymenoptera: Formicidae) from China
title Phylogenetic Relationships Based on DNA Barcoding Among 16 Species of the Ant Genus Formica (Hymenoptera: Formicidae) from China
title_full Phylogenetic Relationships Based on DNA Barcoding Among 16 Species of the Ant Genus Formica (Hymenoptera: Formicidae) from China
title_fullStr Phylogenetic Relationships Based on DNA Barcoding Among 16 Species of the Ant Genus Formica (Hymenoptera: Formicidae) from China
title_full_unstemmed Phylogenetic Relationships Based on DNA Barcoding Among 16 Species of the Ant Genus Formica (Hymenoptera: Formicidae) from China
title_short Phylogenetic Relationships Based on DNA Barcoding Among 16 Species of the Ant Genus Formica (Hymenoptera: Formicidae) from China
title_sort phylogenetic relationships based on dna barcoding among 16 species of the ant genus formica (hymenoptera: formicidae) from china
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5710517/
http://dx.doi.org/10.1093/jisesa/iex092
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