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Evaluation and validation of commercial antibodies for the detection of Shb
Antibodies are among the most important tools for protein detection but, prior to their usage, proper validation of their appropriateness for given applications is required. The utility of an antibody depends on its sensitivity and specificity. We studied these two aspects in a panel of commercial a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5711028/ https://www.ncbi.nlm.nih.gov/pubmed/29194461 http://dx.doi.org/10.1371/journal.pone.0188311 |
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author | Vanli, Güliz Cuesta-Marban, Alvaro Widmann, Christian |
author_facet | Vanli, Güliz Cuesta-Marban, Alvaro Widmann, Christian |
author_sort | Vanli, Güliz |
collection | PubMed |
description | Antibodies are among the most important tools for protein detection but, prior to their usage, proper validation of their appropriateness for given applications is required. The utility of an antibody depends on its sensitivity and specificity. We studied these two aspects in a panel of commercial antibodies against Shb, a platform protein involved in receptor tyrosine kinase signalling, but the function of which is still incompletely understood. Several of the antibodies showed shortcomings or were not acceptable for detection of the endogenous protein. The few that could detect Shb were doing so in either western blotting or immunoprecipitation experiments but a given antibody could not work in both applications. This article provides a resource for the available molecular tools that can be used in future research on Shb. |
format | Online Article Text |
id | pubmed-5711028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57110282017-12-15 Evaluation and validation of commercial antibodies for the detection of Shb Vanli, Güliz Cuesta-Marban, Alvaro Widmann, Christian PLoS One Research Article Antibodies are among the most important tools for protein detection but, prior to their usage, proper validation of their appropriateness for given applications is required. The utility of an antibody depends on its sensitivity and specificity. We studied these two aspects in a panel of commercial antibodies against Shb, a platform protein involved in receptor tyrosine kinase signalling, but the function of which is still incompletely understood. Several of the antibodies showed shortcomings or were not acceptable for detection of the endogenous protein. The few that could detect Shb were doing so in either western blotting or immunoprecipitation experiments but a given antibody could not work in both applications. This article provides a resource for the available molecular tools that can be used in future research on Shb. Public Library of Science 2017-12-01 /pmc/articles/PMC5711028/ /pubmed/29194461 http://dx.doi.org/10.1371/journal.pone.0188311 Text en © 2017 Vanli et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Vanli, Güliz Cuesta-Marban, Alvaro Widmann, Christian Evaluation and validation of commercial antibodies for the detection of Shb |
title | Evaluation and validation of commercial antibodies for the detection of Shb |
title_full | Evaluation and validation of commercial antibodies for the detection of Shb |
title_fullStr | Evaluation and validation of commercial antibodies for the detection of Shb |
title_full_unstemmed | Evaluation and validation of commercial antibodies for the detection of Shb |
title_short | Evaluation and validation of commercial antibodies for the detection of Shb |
title_sort | evaluation and validation of commercial antibodies for the detection of shb |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5711028/ https://www.ncbi.nlm.nih.gov/pubmed/29194461 http://dx.doi.org/10.1371/journal.pone.0188311 |
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