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MicroRNAs activate gene transcription epigenetically as an enhancer trigger

MicroRNAs (miRNAs) are small non-coding RNAs that function as negative gene expression regulators. Emerging evidence shows that, except for function in the cytoplasm, miRNAs are also present in the nucleus. However, the functional significance of nuclear miRNAs remains largely undetermined. By scree...

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Autores principales: Xiao, Min, Li, Jin, Li, Wei, Wang, Yu, Wu, Feizhen, Xi, Yanping, Zhang, Lan, Ding, Chao, Luo, Huaibing, Li, Yan, Peng, Lina, Zhao, Liping, Peng, Shaoliang, Xiao, Yao, Dong, Shihua, Cao, Jie, Yu, Wenqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5711461/
https://www.ncbi.nlm.nih.gov/pubmed/26853707
http://dx.doi.org/10.1080/15476286.2015.1112487
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author Xiao, Min
Li, Jin
Li, Wei
Wang, Yu
Wu, Feizhen
Xi, Yanping
Zhang, Lan
Ding, Chao
Luo, Huaibing
Li, Yan
Peng, Lina
Zhao, Liping
Peng, Shaoliang
Xiao, Yao
Dong, Shihua
Cao, Jie
Yu, Wenqiang
author_facet Xiao, Min
Li, Jin
Li, Wei
Wang, Yu
Wu, Feizhen
Xi, Yanping
Zhang, Lan
Ding, Chao
Luo, Huaibing
Li, Yan
Peng, Lina
Zhao, Liping
Peng, Shaoliang
Xiao, Yao
Dong, Shihua
Cao, Jie
Yu, Wenqiang
author_sort Xiao, Min
collection PubMed
description MicroRNAs (miRNAs) are small non-coding RNAs that function as negative gene expression regulators. Emerging evidence shows that, except for function in the cytoplasm, miRNAs are also present in the nucleus. However, the functional significance of nuclear miRNAs remains largely undetermined. By screening miRNA database, we have identified a subset of miRNA that functions as enhancer regulators. Here, we found a set of miRNAs show gene-activation function. We focused on miR-24-1 and found that this miRNA unconventionally activates gene transcription by targeting enhancers. Consistently, the activation was completely abolished when the enhancer sequence was deleted by TALEN. Furthermore, we found that miR-24-1 activates enhancer RNA (eRNA) expression, alters histone modification, and increases the enrichment of p300 and RNA Pol II at the enhancer locus. Our results demonstrate a novel mechanism of miRNA as an enhancer trigger.
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spelling pubmed-57114612017-12-06 MicroRNAs activate gene transcription epigenetically as an enhancer trigger Xiao, Min Li, Jin Li, Wei Wang, Yu Wu, Feizhen Xi, Yanping Zhang, Lan Ding, Chao Luo, Huaibing Li, Yan Peng, Lina Zhao, Liping Peng, Shaoliang Xiao, Yao Dong, Shihua Cao, Jie Yu, Wenqiang RNA Biol Research Paper MicroRNAs (miRNAs) are small non-coding RNAs that function as negative gene expression regulators. Emerging evidence shows that, except for function in the cytoplasm, miRNAs are also present in the nucleus. However, the functional significance of nuclear miRNAs remains largely undetermined. By screening miRNA database, we have identified a subset of miRNA that functions as enhancer regulators. Here, we found a set of miRNAs show gene-activation function. We focused on miR-24-1 and found that this miRNA unconventionally activates gene transcription by targeting enhancers. Consistently, the activation was completely abolished when the enhancer sequence was deleted by TALEN. Furthermore, we found that miR-24-1 activates enhancer RNA (eRNA) expression, alters histone modification, and increases the enrichment of p300 and RNA Pol II at the enhancer locus. Our results demonstrate a novel mechanism of miRNA as an enhancer trigger. Taylor & Francis 2016-02-06 /pmc/articles/PMC5711461/ /pubmed/26853707 http://dx.doi.org/10.1080/15476286.2015.1112487 Text en © 2017 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Research Paper
Xiao, Min
Li, Jin
Li, Wei
Wang, Yu
Wu, Feizhen
Xi, Yanping
Zhang, Lan
Ding, Chao
Luo, Huaibing
Li, Yan
Peng, Lina
Zhao, Liping
Peng, Shaoliang
Xiao, Yao
Dong, Shihua
Cao, Jie
Yu, Wenqiang
MicroRNAs activate gene transcription epigenetically as an enhancer trigger
title MicroRNAs activate gene transcription epigenetically as an enhancer trigger
title_full MicroRNAs activate gene transcription epigenetically as an enhancer trigger
title_fullStr MicroRNAs activate gene transcription epigenetically as an enhancer trigger
title_full_unstemmed MicroRNAs activate gene transcription epigenetically as an enhancer trigger
title_short MicroRNAs activate gene transcription epigenetically as an enhancer trigger
title_sort micrornas activate gene transcription epigenetically as an enhancer trigger
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5711461/
https://www.ncbi.nlm.nih.gov/pubmed/26853707
http://dx.doi.org/10.1080/15476286.2015.1112487
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