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Molecular Identification of ten species of stored-product psocids through microarray method based on ITS2 rDNA
Stored-product psocids (Psocoptera: Liposcelididae) are cosmopolitan storage pests that can damage stored products and cause serious economic loss. However, because of the body size (~1 mm) of eggs, nymphs, and adults, morphological identification of most stored-product psocids is difficult and hamp...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5711863/ https://www.ncbi.nlm.nih.gov/pubmed/29196710 http://dx.doi.org/10.1038/s41598-017-16888-z |
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author | Liu, Li-Jun Pang, Ao-Han Feng, Shi-Qian Cui, Bing-Yi Zhao, Zi-Hua Kučerová, Zuzana Stejskal, Václav Opit, George Aulicky, Radek Cao, Yang Li, Fu-Jun Wu, Yi Zhang, Tao Li, Zhi-Hong |
author_facet | Liu, Li-Jun Pang, Ao-Han Feng, Shi-Qian Cui, Bing-Yi Zhao, Zi-Hua Kučerová, Zuzana Stejskal, Václav Opit, George Aulicky, Radek Cao, Yang Li, Fu-Jun Wu, Yi Zhang, Tao Li, Zhi-Hong |
author_sort | Liu, Li-Jun |
collection | PubMed |
description | Stored-product psocids (Psocoptera: Liposcelididae) are cosmopolitan storage pests that can damage stored products and cause serious economic loss. However, because of the body size (~1 mm) of eggs, nymphs, and adults, morphological identification of most stored-product psocids is difficult and hampers effective identification. In this study, 10 economically important stored-product Liposcelis spp. psocids (Liposcelis brunnea, L. entomophila, L. decolor, L. pearmani, L. rufa, L.mendax, L. bostrychophila, L. corrodens, L. paeta, and L. tricolor) were collected from 25 geographic locations in 3 countries (China, Czech Republic, and the United States). Ten species-specific probes for identifying these 10 psocid species were designed based on ITS2 sequences. The microarray method and reaction system were optimized. Specificity of each of the ten probes was tested, and all probes were found suitable for use in identification of the respective10 Liposcelis spp. psocids at 66 °C. This method was also used to identify an unknown psocid species collected in Taian, China. This work has contributed to the development of a molecular identification method for stored-product psocids, and can provide technical support not only to facilitate identification of intercepted samples in relation to plant quarantine, but also for use in insect pest monitoring. |
format | Online Article Text |
id | pubmed-5711863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57118632017-12-06 Molecular Identification of ten species of stored-product psocids through microarray method based on ITS2 rDNA Liu, Li-Jun Pang, Ao-Han Feng, Shi-Qian Cui, Bing-Yi Zhao, Zi-Hua Kučerová, Zuzana Stejskal, Václav Opit, George Aulicky, Radek Cao, Yang Li, Fu-Jun Wu, Yi Zhang, Tao Li, Zhi-Hong Sci Rep Article Stored-product psocids (Psocoptera: Liposcelididae) are cosmopolitan storage pests that can damage stored products and cause serious economic loss. However, because of the body size (~1 mm) of eggs, nymphs, and adults, morphological identification of most stored-product psocids is difficult and hampers effective identification. In this study, 10 economically important stored-product Liposcelis spp. psocids (Liposcelis brunnea, L. entomophila, L. decolor, L. pearmani, L. rufa, L.mendax, L. bostrychophila, L. corrodens, L. paeta, and L. tricolor) were collected from 25 geographic locations in 3 countries (China, Czech Republic, and the United States). Ten species-specific probes for identifying these 10 psocid species were designed based on ITS2 sequences. The microarray method and reaction system were optimized. Specificity of each of the ten probes was tested, and all probes were found suitable for use in identification of the respective10 Liposcelis spp. psocids at 66 °C. This method was also used to identify an unknown psocid species collected in Taian, China. This work has contributed to the development of a molecular identification method for stored-product psocids, and can provide technical support not only to facilitate identification of intercepted samples in relation to plant quarantine, but also for use in insect pest monitoring. Nature Publishing Group UK 2017-12-01 /pmc/articles/PMC5711863/ /pubmed/29196710 http://dx.doi.org/10.1038/s41598-017-16888-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Liu, Li-Jun Pang, Ao-Han Feng, Shi-Qian Cui, Bing-Yi Zhao, Zi-Hua Kučerová, Zuzana Stejskal, Václav Opit, George Aulicky, Radek Cao, Yang Li, Fu-Jun Wu, Yi Zhang, Tao Li, Zhi-Hong Molecular Identification of ten species of stored-product psocids through microarray method based on ITS2 rDNA |
title | Molecular Identification of ten species of stored-product psocids through microarray method based on ITS2 rDNA |
title_full | Molecular Identification of ten species of stored-product psocids through microarray method based on ITS2 rDNA |
title_fullStr | Molecular Identification of ten species of stored-product psocids through microarray method based on ITS2 rDNA |
title_full_unstemmed | Molecular Identification of ten species of stored-product psocids through microarray method based on ITS2 rDNA |
title_short | Molecular Identification of ten species of stored-product psocids through microarray method based on ITS2 rDNA |
title_sort | molecular identification of ten species of stored-product psocids through microarray method based on its2 rdna |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5711863/ https://www.ncbi.nlm.nih.gov/pubmed/29196710 http://dx.doi.org/10.1038/s41598-017-16888-z |
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