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MiR-29a Suppresses Spermatogenic Cell Apoptosis in Testicular Ischemia-Reperfusion Injury by Targeting TRPV4 Channels
Background: MicroRNAs (miRNAs) have emerged as gene expression regulators in the progression of ischemia-reperfusion injury (IRI). Accumulating evidences have indicated miR-29a play roles in myocardial and cerebral IRI. However, the role of miR-29a in testicular IRI has not been elucidated. Methods:...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5712590/ https://www.ncbi.nlm.nih.gov/pubmed/29238305 http://dx.doi.org/10.3389/fphys.2017.00966 |
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author | Ning, Jin-zhuo Li, Wei Cheng, Fan Yu, Wei-min Rao, Ting Ruan, Yuan Yuan, Run Zhang, Xiao-bin Zhuo, Dong Du, Yang Xiao, Cheng-cheng |
author_facet | Ning, Jin-zhuo Li, Wei Cheng, Fan Yu, Wei-min Rao, Ting Ruan, Yuan Yuan, Run Zhang, Xiao-bin Zhuo, Dong Du, Yang Xiao, Cheng-cheng |
author_sort | Ning, Jin-zhuo |
collection | PubMed |
description | Background: MicroRNAs (miRNAs) have emerged as gene expression regulators in the progression of ischemia-reperfusion injury (IRI). Accumulating evidences have indicated miR-29a play roles in myocardial and cerebral IRI. However, the role of miR-29a in testicular IRI has not been elucidated. Methods: Changes in expression of miR-29a and Transient Receptor Potential Vanilloid 4 (TRPV4) in animal samples and GC-1 spermatogenic cells were examined. The effects of miR-29a on spermatogenic cell apoptosis in testicular IRI were analyzed both in vitro and in vivo. Results: The expression of MiR-29a was negatively correlated with the expression of TRPV4 and significantly downregulated in animal samples and GC-1 cells as testicular IRI progressed. Further studies revealed TRPV4 as a downstream target of miR-29a. Inhibition of miR-29a expression increased the expression of TRPV4 and promoted spermatogenic cell apoptosis, whereas overexpression of miR-29a downregulated TRPV4 expression and suppressed spermatogenic cell apoptosis caused by testicular IRI in vitro and in vivo. Conclusion: Our results suggest that miR-29a suppresses apoptosis induced by testicular IRI by directly targeting TRPV4. |
format | Online Article Text |
id | pubmed-5712590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57125902017-12-13 MiR-29a Suppresses Spermatogenic Cell Apoptosis in Testicular Ischemia-Reperfusion Injury by Targeting TRPV4 Channels Ning, Jin-zhuo Li, Wei Cheng, Fan Yu, Wei-min Rao, Ting Ruan, Yuan Yuan, Run Zhang, Xiao-bin Zhuo, Dong Du, Yang Xiao, Cheng-cheng Front Physiol Physiology Background: MicroRNAs (miRNAs) have emerged as gene expression regulators in the progression of ischemia-reperfusion injury (IRI). Accumulating evidences have indicated miR-29a play roles in myocardial and cerebral IRI. However, the role of miR-29a in testicular IRI has not been elucidated. Methods: Changes in expression of miR-29a and Transient Receptor Potential Vanilloid 4 (TRPV4) in animal samples and GC-1 spermatogenic cells were examined. The effects of miR-29a on spermatogenic cell apoptosis in testicular IRI were analyzed both in vitro and in vivo. Results: The expression of MiR-29a was negatively correlated with the expression of TRPV4 and significantly downregulated in animal samples and GC-1 cells as testicular IRI progressed. Further studies revealed TRPV4 as a downstream target of miR-29a. Inhibition of miR-29a expression increased the expression of TRPV4 and promoted spermatogenic cell apoptosis, whereas overexpression of miR-29a downregulated TRPV4 expression and suppressed spermatogenic cell apoptosis caused by testicular IRI in vitro and in vivo. Conclusion: Our results suggest that miR-29a suppresses apoptosis induced by testicular IRI by directly targeting TRPV4. Frontiers Media S.A. 2017-11-29 /pmc/articles/PMC5712590/ /pubmed/29238305 http://dx.doi.org/10.3389/fphys.2017.00966 Text en Copyright © 2017 Ning, Li, Cheng, Yu, Rao, Ruan, Yuan, Zhang, Zhuo, Du and Xiao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Ning, Jin-zhuo Li, Wei Cheng, Fan Yu, Wei-min Rao, Ting Ruan, Yuan Yuan, Run Zhang, Xiao-bin Zhuo, Dong Du, Yang Xiao, Cheng-cheng MiR-29a Suppresses Spermatogenic Cell Apoptosis in Testicular Ischemia-Reperfusion Injury by Targeting TRPV4 Channels |
title | MiR-29a Suppresses Spermatogenic Cell Apoptosis in Testicular Ischemia-Reperfusion Injury by Targeting TRPV4 Channels |
title_full | MiR-29a Suppresses Spermatogenic Cell Apoptosis in Testicular Ischemia-Reperfusion Injury by Targeting TRPV4 Channels |
title_fullStr | MiR-29a Suppresses Spermatogenic Cell Apoptosis in Testicular Ischemia-Reperfusion Injury by Targeting TRPV4 Channels |
title_full_unstemmed | MiR-29a Suppresses Spermatogenic Cell Apoptosis in Testicular Ischemia-Reperfusion Injury by Targeting TRPV4 Channels |
title_short | MiR-29a Suppresses Spermatogenic Cell Apoptosis in Testicular Ischemia-Reperfusion Injury by Targeting TRPV4 Channels |
title_sort | mir-29a suppresses spermatogenic cell apoptosis in testicular ischemia-reperfusion injury by targeting trpv4 channels |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5712590/ https://www.ncbi.nlm.nih.gov/pubmed/29238305 http://dx.doi.org/10.3389/fphys.2017.00966 |
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