Cargando…

A Green Microbial Fuel Cell-Based Biosensor for In Situ Chromium (VI) Measurement in Electroplating Wastewater

The extensive use of Cr(VI) in many industries and the disposal of Cr(VI)-containing wastes have resulted in Cr(VI)-induced environmental contamination. Cr(VI) compounds are associated with increased cancer risks; hence, the detection of toxic Cr(VI) compounds is crucial. Various methods have been d...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Li-Chun, Tsai, Teh-Hua, Liu, Man-Hai, Kuo, Jui-Ling, Chang, Yung-Chu, Chung, Ying-Chien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5712896/
https://www.ncbi.nlm.nih.gov/pubmed/29076985
http://dx.doi.org/10.3390/s17112461
_version_ 1783283311211184128
author Wu, Li-Chun
Tsai, Teh-Hua
Liu, Man-Hai
Kuo, Jui-Ling
Chang, Yung-Chu
Chung, Ying-Chien
author_facet Wu, Li-Chun
Tsai, Teh-Hua
Liu, Man-Hai
Kuo, Jui-Ling
Chang, Yung-Chu
Chung, Ying-Chien
author_sort Wu, Li-Chun
collection PubMed
description The extensive use of Cr(VI) in many industries and the disposal of Cr(VI)-containing wastes have resulted in Cr(VI)-induced environmental contamination. Cr(VI) compounds are associated with increased cancer risks; hence, the detection of toxic Cr(VI) compounds is crucial. Various methods have been developed for Cr(VI) measurement, but they are often conducted offsite and cannot provide real-time toxicity monitoring. A microbial fuel cell (MFC) is an eco-friendly and self-sustaining device that has great potential as a biosensor for in situ Cr(VI) measurement, especially for wastewater generated from different electroplating units. In this study, Exiguobacterium aestuarii YC211, a facultatively anaerobic, Cr(VI)-reducing, salt-tolerant, and exoelectrogenic bacterium, was isolated and inoculated into an MFC to evaluate its feasibility as a Cr(VI) biosensor. The Cr(VI) removal efficiency of E. aestuarii YC211 was not affected by the surrounding environment (pH 5–9, 20–35 °C, coexisting ions, and salinity of 0–15 g/L). The maximum power density of the MFC biosensor was 98.3 ± 1.5 mW/m(2) at 1500 Ω. A good linear relationship (r(2) = 0.997) was observed between the Cr(VI) concentration (2.5–60 mg/L) and the voltage output. The developed MFC biosensor is a simple device that can accurately measure Cr(VI) concentrations in the actual electroplating wastewater that is generated from different electroplating units within 30 min with low deviations (−6.1% to 2.2%). After treating the actual electroplating wastewater with the MFC, the predominant family in the biofilm was found to be Bacillaceae (95.3%) and was further identified as the originally inoculated E. aestuarii YC211 by next generation sequencing (NGS). Thus, the MFC biosensor can measure Cr(VI) concentrations in situ in the effluents from different electroplating units, and it can potentially help in preventing the violation of effluent regulations.
format Online
Article
Text
id pubmed-5712896
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-57128962017-12-07 A Green Microbial Fuel Cell-Based Biosensor for In Situ Chromium (VI) Measurement in Electroplating Wastewater Wu, Li-Chun Tsai, Teh-Hua Liu, Man-Hai Kuo, Jui-Ling Chang, Yung-Chu Chung, Ying-Chien Sensors (Basel) Article The extensive use of Cr(VI) in many industries and the disposal of Cr(VI)-containing wastes have resulted in Cr(VI)-induced environmental contamination. Cr(VI) compounds are associated with increased cancer risks; hence, the detection of toxic Cr(VI) compounds is crucial. Various methods have been developed for Cr(VI) measurement, but they are often conducted offsite and cannot provide real-time toxicity monitoring. A microbial fuel cell (MFC) is an eco-friendly and self-sustaining device that has great potential as a biosensor for in situ Cr(VI) measurement, especially for wastewater generated from different electroplating units. In this study, Exiguobacterium aestuarii YC211, a facultatively anaerobic, Cr(VI)-reducing, salt-tolerant, and exoelectrogenic bacterium, was isolated and inoculated into an MFC to evaluate its feasibility as a Cr(VI) biosensor. The Cr(VI) removal efficiency of E. aestuarii YC211 was not affected by the surrounding environment (pH 5–9, 20–35 °C, coexisting ions, and salinity of 0–15 g/L). The maximum power density of the MFC biosensor was 98.3 ± 1.5 mW/m(2) at 1500 Ω. A good linear relationship (r(2) = 0.997) was observed between the Cr(VI) concentration (2.5–60 mg/L) and the voltage output. The developed MFC biosensor is a simple device that can accurately measure Cr(VI) concentrations in the actual electroplating wastewater that is generated from different electroplating units within 30 min with low deviations (−6.1% to 2.2%). After treating the actual electroplating wastewater with the MFC, the predominant family in the biofilm was found to be Bacillaceae (95.3%) and was further identified as the originally inoculated E. aestuarii YC211 by next generation sequencing (NGS). Thus, the MFC biosensor can measure Cr(VI) concentrations in situ in the effluents from different electroplating units, and it can potentially help in preventing the violation of effluent regulations. MDPI 2017-10-27 /pmc/articles/PMC5712896/ /pubmed/29076985 http://dx.doi.org/10.3390/s17112461 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Li-Chun
Tsai, Teh-Hua
Liu, Man-Hai
Kuo, Jui-Ling
Chang, Yung-Chu
Chung, Ying-Chien
A Green Microbial Fuel Cell-Based Biosensor for In Situ Chromium (VI) Measurement in Electroplating Wastewater
title A Green Microbial Fuel Cell-Based Biosensor for In Situ Chromium (VI) Measurement in Electroplating Wastewater
title_full A Green Microbial Fuel Cell-Based Biosensor for In Situ Chromium (VI) Measurement in Electroplating Wastewater
title_fullStr A Green Microbial Fuel Cell-Based Biosensor for In Situ Chromium (VI) Measurement in Electroplating Wastewater
title_full_unstemmed A Green Microbial Fuel Cell-Based Biosensor for In Situ Chromium (VI) Measurement in Electroplating Wastewater
title_short A Green Microbial Fuel Cell-Based Biosensor for In Situ Chromium (VI) Measurement in Electroplating Wastewater
title_sort green microbial fuel cell-based biosensor for in situ chromium (vi) measurement in electroplating wastewater
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5712896/
https://www.ncbi.nlm.nih.gov/pubmed/29076985
http://dx.doi.org/10.3390/s17112461
work_keys_str_mv AT wulichun agreenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater
AT tsaitehhua agreenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater
AT liumanhai agreenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater
AT kuojuiling agreenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater
AT changyungchu agreenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater
AT chungyingchien agreenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater
AT wulichun greenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater
AT tsaitehhua greenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater
AT liumanhai greenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater
AT kuojuiling greenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater
AT changyungchu greenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater
AT chungyingchien greenmicrobialfuelcellbasedbiosensorforinsituchromiumvimeasurementinelectroplatingwastewater