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Water-Based Suspensions of Iron Oxide Nanoparticles with Electrostatic or Steric Stabilization by Chitosan: Fabrication, Characterization and Biocompatibility

Present day biomedical applications, including magnetic biosensing, demand better understanding of the interactions between living systems and magnetic nanoparticles (MNPs). In this work spherical MNPs of maghemite were obtained by a highly productive laser target evaporation technique. XRD analysis...

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Autores principales: Kurlyandskaya, Galina V., Litvinova, Larisa S., Safronov, Alexander P., Schupletsova, Valeria V., Tyukova, Irina S., Khaziakhmatova, Olga G., Slepchenko, Galina B., Yurova, Kristina A., Cherempey, Elena G., Kulesh, Nikita A., Andrade, Ricardo, Beketov, Igor V., Khlusov, Igor A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5712992/
https://www.ncbi.nlm.nih.gov/pubmed/29137198
http://dx.doi.org/10.3390/s17112605
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author Kurlyandskaya, Galina V.
Litvinova, Larisa S.
Safronov, Alexander P.
Schupletsova, Valeria V.
Tyukova, Irina S.
Khaziakhmatova, Olga G.
Slepchenko, Galina B.
Yurova, Kristina A.
Cherempey, Elena G.
Kulesh, Nikita A.
Andrade, Ricardo
Beketov, Igor V.
Khlusov, Igor A.
author_facet Kurlyandskaya, Galina V.
Litvinova, Larisa S.
Safronov, Alexander P.
Schupletsova, Valeria V.
Tyukova, Irina S.
Khaziakhmatova, Olga G.
Slepchenko, Galina B.
Yurova, Kristina A.
Cherempey, Elena G.
Kulesh, Nikita A.
Andrade, Ricardo
Beketov, Igor V.
Khlusov, Igor A.
author_sort Kurlyandskaya, Galina V.
collection PubMed
description Present day biomedical applications, including magnetic biosensing, demand better understanding of the interactions between living systems and magnetic nanoparticles (MNPs). In this work spherical MNPs of maghemite were obtained by a highly productive laser target evaporation technique. XRD analysis confirmed the inverse spinel structure of the MNPs (space group Fd-3m). The ensemble obeyed a lognormal size distribution with the median value 26.8 nm and dispersion 0.362. Stabilized water-based suspensions were fabricated using electrostatic or steric stabilization by the natural polymer chitosan. The encapsulation of the MNPs by chitosan makes them resistant to the unfavorable factors for colloidal stability typically present in physiological conditions such as pH and high ionic force. Controlled amounts of suspensions were used for in vitro experiments with human blood mononuclear leukocytes (HBMLs) in order to study their morphofunctional response. For sake of comparison the results obtained in the present study were analyzed together with our previous results of the study of similar suspensions with human mesenchymal stem cells. Suspensions with and without chitosan enhanced the secretion of cytokines by a 24-h culture of HBMLs compared to a control without MNPs. At a dose of 2.3, the MTD of chitosan promotes the stimulating effect of MNPs on cells. In the dose range of MNPs 10–1000 MTD, chitosan “inhibits” cellular secretory activity compared to MNPs without chitosan. Both suspensions did not caused cell death by necrosis, hence, the secretion of cytokines is due to the enhancement of the functional activity of HBMLs. Increased accumulation of MNP with chitosan in the cell fraction at 100 MTD for 24 h exposure, may be due to fixation of chitosan on the outer membrane of HBMLs. The discussed results can be used for an addressed design of cell delivery/removal incorporating multiple activities because of cell capability to avoid phagocytosis by immune cells. They are also promising for the field of biosensor development for the detection of magnetic labels.
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spelling pubmed-57129922017-12-07 Water-Based Suspensions of Iron Oxide Nanoparticles with Electrostatic or Steric Stabilization by Chitosan: Fabrication, Characterization and Biocompatibility Kurlyandskaya, Galina V. Litvinova, Larisa S. Safronov, Alexander P. Schupletsova, Valeria V. Tyukova, Irina S. Khaziakhmatova, Olga G. Slepchenko, Galina B. Yurova, Kristina A. Cherempey, Elena G. Kulesh, Nikita A. Andrade, Ricardo Beketov, Igor V. Khlusov, Igor A. Sensors (Basel) Article Present day biomedical applications, including magnetic biosensing, demand better understanding of the interactions between living systems and magnetic nanoparticles (MNPs). In this work spherical MNPs of maghemite were obtained by a highly productive laser target evaporation technique. XRD analysis confirmed the inverse spinel structure of the MNPs (space group Fd-3m). The ensemble obeyed a lognormal size distribution with the median value 26.8 nm and dispersion 0.362. Stabilized water-based suspensions were fabricated using electrostatic or steric stabilization by the natural polymer chitosan. The encapsulation of the MNPs by chitosan makes them resistant to the unfavorable factors for colloidal stability typically present in physiological conditions such as pH and high ionic force. Controlled amounts of suspensions were used for in vitro experiments with human blood mononuclear leukocytes (HBMLs) in order to study their morphofunctional response. For sake of comparison the results obtained in the present study were analyzed together with our previous results of the study of similar suspensions with human mesenchymal stem cells. Suspensions with and without chitosan enhanced the secretion of cytokines by a 24-h culture of HBMLs compared to a control without MNPs. At a dose of 2.3, the MTD of chitosan promotes the stimulating effect of MNPs on cells. In the dose range of MNPs 10–1000 MTD, chitosan “inhibits” cellular secretory activity compared to MNPs without chitosan. Both suspensions did not caused cell death by necrosis, hence, the secretion of cytokines is due to the enhancement of the functional activity of HBMLs. Increased accumulation of MNP with chitosan in the cell fraction at 100 MTD for 24 h exposure, may be due to fixation of chitosan on the outer membrane of HBMLs. The discussed results can be used for an addressed design of cell delivery/removal incorporating multiple activities because of cell capability to avoid phagocytosis by immune cells. They are also promising for the field of biosensor development for the detection of magnetic labels. MDPI 2017-11-13 /pmc/articles/PMC5712992/ /pubmed/29137198 http://dx.doi.org/10.3390/s17112605 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kurlyandskaya, Galina V.
Litvinova, Larisa S.
Safronov, Alexander P.
Schupletsova, Valeria V.
Tyukova, Irina S.
Khaziakhmatova, Olga G.
Slepchenko, Galina B.
Yurova, Kristina A.
Cherempey, Elena G.
Kulesh, Nikita A.
Andrade, Ricardo
Beketov, Igor V.
Khlusov, Igor A.
Water-Based Suspensions of Iron Oxide Nanoparticles with Electrostatic or Steric Stabilization by Chitosan: Fabrication, Characterization and Biocompatibility
title Water-Based Suspensions of Iron Oxide Nanoparticles with Electrostatic or Steric Stabilization by Chitosan: Fabrication, Characterization and Biocompatibility
title_full Water-Based Suspensions of Iron Oxide Nanoparticles with Electrostatic or Steric Stabilization by Chitosan: Fabrication, Characterization and Biocompatibility
title_fullStr Water-Based Suspensions of Iron Oxide Nanoparticles with Electrostatic or Steric Stabilization by Chitosan: Fabrication, Characterization and Biocompatibility
title_full_unstemmed Water-Based Suspensions of Iron Oxide Nanoparticles with Electrostatic or Steric Stabilization by Chitosan: Fabrication, Characterization and Biocompatibility
title_short Water-Based Suspensions of Iron Oxide Nanoparticles with Electrostatic or Steric Stabilization by Chitosan: Fabrication, Characterization and Biocompatibility
title_sort water-based suspensions of iron oxide nanoparticles with electrostatic or steric stabilization by chitosan: fabrication, characterization and biocompatibility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5712992/
https://www.ncbi.nlm.nih.gov/pubmed/29137198
http://dx.doi.org/10.3390/s17112605
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