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Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains
Low pathogenic avian influenza (LPAI) viruses are a source of sporadic human infections and could also contribute to future pandemic outbreaks but little is known about inter-species differences in the host responses to these viruses. Here, we studied host gene expression signatures of cell lines fr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713265/ https://www.ncbi.nlm.nih.gov/pubmed/29104227 http://dx.doi.org/10.3390/ijms18112295 |
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author | Taye, Biruhalem Yeo, Dawn Lee, Raphael Tze Chuen Tan, Boon Huan Sugrue, Richard J. Maurer-Stroh, Sebastian |
author_facet | Taye, Biruhalem Yeo, Dawn Lee, Raphael Tze Chuen Tan, Boon Huan Sugrue, Richard J. Maurer-Stroh, Sebastian |
author_sort | Taye, Biruhalem |
collection | PubMed |
description | Low pathogenic avian influenza (LPAI) viruses are a source of sporadic human infections and could also contribute to future pandemic outbreaks but little is known about inter-species differences in the host responses to these viruses. Here, we studied host gene expression signatures of cell lines from three species (human, chicken, and canine) in response to six different viruses (H1N1/WSN, H5N2/F59, H5N2/F118, H5N2/F189, H5N3 and H9N2). Comprehensive microarray probe set re-annotation and ortholog mapping of the host genes was necessary to allow comparison over extended functionally annotated gene sets and orthologous pathways. The annotations are made available to the community for commonly used microarray chips. We observe a strong tendency of the response being cell type- rather than virus-specific. In chicken cells, we found up-regulation of host factors inducing virus infectivity (e.g., oxysterol binding protein like 1A (OSBPL1A) and Rho GTPase activating protein 21 (ARHGAP21)) while reducing apoptosis (e.g., mitochondrial ribosomal protein S27 (MRPS27)) and increasing cell proliferation (e.g., COP9 signalosome subunit 2 (COPS2)). On the other hand, increased antiviral, pro-apoptotic and inflammatory signatures have been identified in human cells while cell cycle and metabolic pathways were down-regulated. This signature describes how low pathogenic avian influenza (LPAI) viruses are being tolerated and shed from chicken but potentially causing cellular disruption in mammalian cells. |
format | Online Article Text |
id | pubmed-5713265 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57132652017-12-07 Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains Taye, Biruhalem Yeo, Dawn Lee, Raphael Tze Chuen Tan, Boon Huan Sugrue, Richard J. Maurer-Stroh, Sebastian Int J Mol Sci Article Low pathogenic avian influenza (LPAI) viruses are a source of sporadic human infections and could also contribute to future pandemic outbreaks but little is known about inter-species differences in the host responses to these viruses. Here, we studied host gene expression signatures of cell lines from three species (human, chicken, and canine) in response to six different viruses (H1N1/WSN, H5N2/F59, H5N2/F118, H5N2/F189, H5N3 and H9N2). Comprehensive microarray probe set re-annotation and ortholog mapping of the host genes was necessary to allow comparison over extended functionally annotated gene sets and orthologous pathways. The annotations are made available to the community for commonly used microarray chips. We observe a strong tendency of the response being cell type- rather than virus-specific. In chicken cells, we found up-regulation of host factors inducing virus infectivity (e.g., oxysterol binding protein like 1A (OSBPL1A) and Rho GTPase activating protein 21 (ARHGAP21)) while reducing apoptosis (e.g., mitochondrial ribosomal protein S27 (MRPS27)) and increasing cell proliferation (e.g., COP9 signalosome subunit 2 (COPS2)). On the other hand, increased antiviral, pro-apoptotic and inflammatory signatures have been identified in human cells while cell cycle and metabolic pathways were down-regulated. This signature describes how low pathogenic avian influenza (LPAI) viruses are being tolerated and shed from chicken but potentially causing cellular disruption in mammalian cells. MDPI 2017-11-01 /pmc/articles/PMC5713265/ /pubmed/29104227 http://dx.doi.org/10.3390/ijms18112295 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Taye, Biruhalem Yeo, Dawn Lee, Raphael Tze Chuen Tan, Boon Huan Sugrue, Richard J. Maurer-Stroh, Sebastian Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains |
title | Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains |
title_full | Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains |
title_fullStr | Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains |
title_full_unstemmed | Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains |
title_short | Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains |
title_sort | inter-species host gene expression differences in response to human and avian influenza a virus strains |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713265/ https://www.ncbi.nlm.nih.gov/pubmed/29104227 http://dx.doi.org/10.3390/ijms18112295 |
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