Cargando…

Recipient HLA-G +3142 CC Genotype and Concentrations of Soluble HLA-G Impact on Occurrence of CMV Infection after Living-Donor Kidney Transplantation

The expression modulation of the immunosuppressive non-classical Human leukocyte antigen-G (HLA-G) molecule and its soluble isoforms is an immune evasion strategy being deployed by cytomegalovirus (CMV). The +3142 C>G single nucleotide polymorphism (SNP) located within the 3′ untranslated region...

Descripción completa

Detalles Bibliográficos
Autores principales: Guberina, Hana, Tomoya Michita, Rafael, Dolff, Sebastian, Bienholz, Anja, Trilling, Mirko, Heinemann, Falko M., Horn, Peter A., Kribben, Andreas, Witzke, Oliver, Rebmann, Vera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713307/
https://www.ncbi.nlm.nih.gov/pubmed/29113092
http://dx.doi.org/10.3390/ijms18112338
_version_ 1783283394162982912
author Guberina, Hana
Tomoya Michita, Rafael
Dolff, Sebastian
Bienholz, Anja
Trilling, Mirko
Heinemann, Falko M.
Horn, Peter A.
Kribben, Andreas
Witzke, Oliver
Rebmann, Vera
author_facet Guberina, Hana
Tomoya Michita, Rafael
Dolff, Sebastian
Bienholz, Anja
Trilling, Mirko
Heinemann, Falko M.
Horn, Peter A.
Kribben, Andreas
Witzke, Oliver
Rebmann, Vera
author_sort Guberina, Hana
collection PubMed
description The expression modulation of the immunosuppressive non-classical Human leukocyte antigen-G (HLA-G) molecule and its soluble isoforms is an immune evasion strategy being deployed by cytomegalovirus (CMV). The +3142 C>G single nucleotide polymorphism (SNP) located within the 3′ untranslated region (3′UTR) is of crucial importance for the regulation of HLA-G expression. Therefore, we analyzed the influence of the +3142 C>G HLA-G SNP on the occurrence of CMV infection in a cohort of 178 living-donor kidney recipients and their 178 corresponding donors. In addition, soluble HLA-G (sHLA-G) levels were quantified before and after transplantation. The presence of the HLA-G +3142 CC genotype in recipients, but not donors of our cohort as along with elevated sHLA-G levels (≥6.1 ng/mL) were associated with higher susceptibility to CMV infection after transplantation. Our results provided evidence that (i) HLA-G is implicated in the establishment of CMV after living-donor kidney transplantation and (ii) recipient HLA-G +3142 CC genotype and sHLA-G concentration levels could represent important predictive risk markers for CMV infection.
format Online
Article
Text
id pubmed-5713307
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-57133072017-12-07 Recipient HLA-G +3142 CC Genotype and Concentrations of Soluble HLA-G Impact on Occurrence of CMV Infection after Living-Donor Kidney Transplantation Guberina, Hana Tomoya Michita, Rafael Dolff, Sebastian Bienholz, Anja Trilling, Mirko Heinemann, Falko M. Horn, Peter A. Kribben, Andreas Witzke, Oliver Rebmann, Vera Int J Mol Sci Article The expression modulation of the immunosuppressive non-classical Human leukocyte antigen-G (HLA-G) molecule and its soluble isoforms is an immune evasion strategy being deployed by cytomegalovirus (CMV). The +3142 C>G single nucleotide polymorphism (SNP) located within the 3′ untranslated region (3′UTR) is of crucial importance for the regulation of HLA-G expression. Therefore, we analyzed the influence of the +3142 C>G HLA-G SNP on the occurrence of CMV infection in a cohort of 178 living-donor kidney recipients and their 178 corresponding donors. In addition, soluble HLA-G (sHLA-G) levels were quantified before and after transplantation. The presence of the HLA-G +3142 CC genotype in recipients, but not donors of our cohort as along with elevated sHLA-G levels (≥6.1 ng/mL) were associated with higher susceptibility to CMV infection after transplantation. Our results provided evidence that (i) HLA-G is implicated in the establishment of CMV after living-donor kidney transplantation and (ii) recipient HLA-G +3142 CC genotype and sHLA-G concentration levels could represent important predictive risk markers for CMV infection. MDPI 2017-11-05 /pmc/articles/PMC5713307/ /pubmed/29113092 http://dx.doi.org/10.3390/ijms18112338 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guberina, Hana
Tomoya Michita, Rafael
Dolff, Sebastian
Bienholz, Anja
Trilling, Mirko
Heinemann, Falko M.
Horn, Peter A.
Kribben, Andreas
Witzke, Oliver
Rebmann, Vera
Recipient HLA-G +3142 CC Genotype and Concentrations of Soluble HLA-G Impact on Occurrence of CMV Infection after Living-Donor Kidney Transplantation
title Recipient HLA-G +3142 CC Genotype and Concentrations of Soluble HLA-G Impact on Occurrence of CMV Infection after Living-Donor Kidney Transplantation
title_full Recipient HLA-G +3142 CC Genotype and Concentrations of Soluble HLA-G Impact on Occurrence of CMV Infection after Living-Donor Kidney Transplantation
title_fullStr Recipient HLA-G +3142 CC Genotype and Concentrations of Soluble HLA-G Impact on Occurrence of CMV Infection after Living-Donor Kidney Transplantation
title_full_unstemmed Recipient HLA-G +3142 CC Genotype and Concentrations of Soluble HLA-G Impact on Occurrence of CMV Infection after Living-Donor Kidney Transplantation
title_short Recipient HLA-G +3142 CC Genotype and Concentrations of Soluble HLA-G Impact on Occurrence of CMV Infection after Living-Donor Kidney Transplantation
title_sort recipient hla-g +3142 cc genotype and concentrations of soluble hla-g impact on occurrence of cmv infection after living-donor kidney transplantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713307/
https://www.ncbi.nlm.nih.gov/pubmed/29113092
http://dx.doi.org/10.3390/ijms18112338
work_keys_str_mv AT guberinahana recipienthlag3142ccgenotypeandconcentrationsofsolublehlagimpactonoccurrenceofcmvinfectionafterlivingdonorkidneytransplantation
AT tomoyamichitarafael recipienthlag3142ccgenotypeandconcentrationsofsolublehlagimpactonoccurrenceofcmvinfectionafterlivingdonorkidneytransplantation
AT dolffsebastian recipienthlag3142ccgenotypeandconcentrationsofsolublehlagimpactonoccurrenceofcmvinfectionafterlivingdonorkidneytransplantation
AT bienholzanja recipienthlag3142ccgenotypeandconcentrationsofsolublehlagimpactonoccurrenceofcmvinfectionafterlivingdonorkidneytransplantation
AT trillingmirko recipienthlag3142ccgenotypeandconcentrationsofsolublehlagimpactonoccurrenceofcmvinfectionafterlivingdonorkidneytransplantation
AT heinemannfalkom recipienthlag3142ccgenotypeandconcentrationsofsolublehlagimpactonoccurrenceofcmvinfectionafterlivingdonorkidneytransplantation
AT hornpetera recipienthlag3142ccgenotypeandconcentrationsofsolublehlagimpactonoccurrenceofcmvinfectionafterlivingdonorkidneytransplantation
AT kribbenandreas recipienthlag3142ccgenotypeandconcentrationsofsolublehlagimpactonoccurrenceofcmvinfectionafterlivingdonorkidneytransplantation
AT witzkeoliver recipienthlag3142ccgenotypeandconcentrationsofsolublehlagimpactonoccurrenceofcmvinfectionafterlivingdonorkidneytransplantation
AT rebmannvera recipienthlag3142ccgenotypeandconcentrationsofsolublehlagimpactonoccurrenceofcmvinfectionafterlivingdonorkidneytransplantation